C57BL/6JCya-Il22ra2em1flox/Cya
Common Name:
Il22ra2-flox
Product ID:
S-CKO-07915
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Il22ra2-flox
Strain ID
CKOCMP-237310-Il22ra2-B6J-VA
Gene Name
Product ID
S-CKO-07915
Gene Alias
CRF2-10; CRF2-s1; CRF2X; Il-22bp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il22ra2em1flox/Cya mice (Catalog S-CKO-07915) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036564
NCBI RefSeq
NM_178258
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Overview of Gene Research
Il22ra2, encoding the IL-22 binding protein (IL-22BP), is a gene with significant biological importance. IL-22BP is a soluble decoy that decreases the bioavailability of interleukin 22 (IL-22), thus attenuating IL-22 signaling. IL-22 is involved in promoting intestinal barrier integrity and stimulating epithelial cells to defend against enteric infections, so Il22ra2 indirectly impacts these processes [1].
In gene knockout studies, Il22ra2-/-mice show enhanced protection against Clostridioides difficile and Citrobacter rodentium infections. This protection is due to IL-22-induced antimicrobial mechanisms before infection, and the gut microbiota of these knockout mice can mitigate infection in wild-type mice upon transfer. Resistance to C. difficile infection is related to increased acetate production by Il22ra2-/-associated microbiota [1].
In the context of multiple sclerosis, a rare coding variant rs28385692 in the IL22RA2 signal peptide coding region decreases the secretion of IL-22BP isoforms and is associated with an augmented risk for the disease [2]. Also, Il22ra2-/-mice have a less severe course of experimental autoimmune encephalomyelitis, with less demyelination and immune cell infiltration in the central nervous system, suggesting that lack of IL-22BP leads to higher IL-22 availability which has a protective effect in CNS inflammation [3].
In pneumococcal pneumonia, Il22ra2 deficiency favors down-regulation of oxidative phosphorylation genes in an IL-22-dependent manner, and Il22ra2-/-mice are more resistant to the infection [4].
In the bleomycin model of acute lung injury, Il22ra2-/-mice have greater sensitivity and pulmonary inflammation in the acute phase, driven by excess IL-22 production and the influx of pathogenic IL-17A+ γδ T cells to the lung [5].
In conclusion, Il22ra2 plays a crucial role in modulating IL-22 bioavailability and subsequent signaling, influencing host defense mechanisms. Gene knockout mouse models have revealed its significance in various disease areas such as enteric infections, multiple sclerosis, neuroinflammation, pneumococcal pneumonia, and acute lung injury. Understanding Il22ra2 can provide insights into disease mechanisms and potential therapeutic strategies.
References：
1. Fachi, José L, Di Luccia, Blanda, Gilfillan, Susan, Gordon, Jeffrey I, Colonna, Marco. 2024. Deficiency of IL-22-binding protein enhances the ability of the gut microbiota to protect against enteric pathogens. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2321836121. doi:10.1073/pnas.2321836121. https://pubmed.ncbi.nlm.nih.gov/38687788/
2. Gómez-Fernández, Paloma, Lopez de Lapuente Portilla, Aitzkoa, Astobiza, Ianire, Lill, Christina M, Vandenbroeck, Koen. 2020. The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis. In Cells, 9, . doi:10.3390/cells9010175. https://pubmed.ncbi.nlm.nih.gov/31936765/
3. Laaksonen, H, Guerreiro-Cacais, A O, Adzemovic, M Z, Jagodic, M, Olsson, T. 2014. The multiple sclerosis risk gene IL22RA2 contributes to a more severe murine autoimmune neuroinflammation. In Genes and immunity, 15, 457-65. doi:10.1038/gene.2014.36. https://pubmed.ncbi.nlm.nih.gov/25008863/
4. Trevejo-Nunez, Giraldina, Elsegeiny, Waleed, Aggor, Felix E Y, Kolls, Jay K, Gaffen, Sarah L. 2019. Interleukin-22 (IL-22) Binding Protein Constrains IL-22 Activity, Host Defense, and Oxidative Phosphorylation Genes during Pneumococcal Pneumonia. In Infection and immunity, 87, . doi:10.1128/IAI.00550-19. https://pubmed.ncbi.nlm.nih.gov/31451621/
5. Zhang, Zhe, Chakawa, Mazvita B, Galeas-Pena, Michelle, Jones, MaryJane, Pociask, Derek. 2023. IL-22 Binding Protein Controls IL-22-Driven Bleomycin-Induced Lung Injury. In The American journal of pathology, 194, 338-352. doi:10.1016/j.ajpath.2023.11.011. https://pubmed.ncbi.nlm.nih.gov/38101567/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen