C57BL/6JCya-Homer1em1flox/Cya
Common Name
Homer1-flox
Product ID
S-CKO-09617
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-26556-Homer1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Homer1-flox Mouse (Catalog S-CKO-09617) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Homer1-flox
Strain ID
CKOCMP-26556-Homer1-B6J-VA
Gene Name
Product ID
S-CKO-09617
Gene Alias
SYN47, Ves-1, vesl-1, homer-1, PSD-Zip45
Background
C57BL/6JCya
Gene Full Name
homer scaffolding protein 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 13 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000079086
NCBI Transcript ID
NM_152134
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Overview of Gene Research
Homer1, a synaptic scaffold protein, is crucial for regulating glutamatergic synapses and spine morphogenesis [4]. It interacts with various proteins like metabotropic glutamate receptors (mGluRs), modulating calcium signaling, long-term potentiation, and dendritic spine growth. It also plays a part in integrating different signal transduction pathways within the postsynaptic density, being involved in motor and cognitive functions [2].
In disease-related studies, Homer1 has shown significant impacts. In intracerebral hemorrhage (ICH) models, overexpression of Homer1 promotes the conversion of A1 astrocytes (associated with neuroinflammation) to A2 astrocytes (neuroprotective), inhibits MAPK signaling activation, and improves outcomes, associated with changes in inflammatory and anti-inflammatory factors [1]. In middle cerebral artery occlusion-induced retinal ischemia models, Homer1 overexpression protects retinal ganglion cells from pyroptosis by inhibiting endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation [3]. In ischemic stroke models, knockdown of Homer1 promotes neuronal death by enhancing necroptotic signaling and inflammation, while injection of Homer1 protein reduces injury and improves outcomes [5]. In chronic social stress models, disruption of Homer1/mGluR5 coupling is observed, and Homer1 knockout mice show hyperactivity and hypothalamic-pituitary-adrenal axis dysregulation [6].
In conclusion, Homer1 is essential for synaptic function and spine stability. Through gene knockout (KO) and other genetic models, its role in various disease conditions such as ICH, retinal ischemia, ischemic stroke, and stress-induced psychiatric disorders has been revealed. These models have provided insights into potential therapeutic targets related to Homer1 in treating these diseases.
References：
1. Fei, Xiaowei, Dou, Ya-Nan, Wang, Li, Wei, Jialiang, Fei, Zhou. 2022. Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage. In Journal of neuroinflammation, 19, 67. doi:10.1186/s12974-022-02428-8. https://pubmed.ncbi.nlm.nih.gov/35287697/
2. de Bartolomeis, Andrea, Barone, Annarita, Buonaguro, Elisabetta Filomena, Vellucci, Licia, Iasevoli, Felice. 2022. The Homer1 family of proteins at the crossroad of dopamine-glutamate signaling: An emerging molecular "Lego" in the pathophysiology of psychiatric disorders. A systematic review and translational insight. In Neuroscience and biobehavioral reviews, 136, 104596. doi:10.1016/j.neubiorev.2022.104596. https://pubmed.ncbi.nlm.nih.gov/35248676/
3. Lv, Weihao, Wu, Xiuquan, Dou, Yanan, Fei, Zhou, Fei, Fei. 2023. Homer1 Protects against Retinal Ganglion Cell Pyroptosis by Inhibiting Endoplasmic Reticulum Stress-Associated TXNIP/NLRP3 Inflammasome Activation after Middle Cerebral Artery Occlusion-Induced Retinal Ischemia. In International journal of molecular sciences, 24, . doi:10.3390/ijms242316811. https://pubmed.ncbi.nlm.nih.gov/38069134/
4. Yoon, Sehyoun, Piguel, Nicolas H, Khalatyan, Natalia, Savas, Jeffrey N, Penzes, Peter. 2021. Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome. In Molecular psychiatry, 26, 1775-1789. doi:10.1038/s41380-020-00991-1. https://pubmed.ncbi.nlm.nih.gov/33398084/
5. Lv, Weihao, Zhang, Qianqian, Li, Yuanming, Zhang, Lei, Fei, Zhou. 2023. Homer1 ameliorates ischemic stroke by inhibiting necroptosis-induced neuronal damage and neuroinflammation. In Inflammation research : official journal of the European Histamine Research Society ... [et al.], 73, 131-144. doi:10.1007/s00011-023-01824-x. https://pubmed.ncbi.nlm.nih.gov/38091015/
6. Wagner, Klaus V, Hartmann, Jakob, Labermaier, Christiana, Müller, Marianne B, Schmidt, Mathias V. 2015. Homer1/mGluR5 activity moderates vulnerability to chronic social stress. In Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 40, 1222-33. doi:10.1038/npp.2014.308. https://pubmed.ncbi.nlm.nih.gov/25409593/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
