C57BL/6JCya-Rinlem1flox/Cya
Common Name:
Rinl-flox
Product ID:
S-CKO-10439
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rinl-flox
Strain ID
CKOCMP-320435-Rinl-B6J-VA
Gene Name
Product ID
S-CKO-10439
Gene Alias
5830482F20Rik; 9930116N10Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rinlem1flox/Cya mice (Catalog S-CKO-10439) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059857
NCBI RefSeq
NM_177158
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Rinl, also known as Ras and Rab interactor (or Ras interaction/interference)-like, is a guanine nucleotide exchange factor (GEF) for the Rab5 subfamily proteins. It contains a conserved VPS9 domain crucial for GEF action and is involved in membrane trafficking. Rinl is implicated in the EphA8-degradation pathway via its interaction with odin and also plays a role in regulating Tfh cell differentiation by controlling CD28 internalization and signaling. It is also associated with endocytotic processes regulated by Rab5a and Rab22 [1,2,3].
In mice, loss of Rinl leads to an increase of Tfh cells in aging, upon in vivo immunization, and acute LCMV Armstrong infection, indicating its role as a negative regulator of Tfh generation. This reveals its significance in shaping CD4+ T cell activation and differentiation [2].
In conclusion, Rinl is a key GEF that is involved in multiple biological processes, including protein degradation pathways, Tfh cell differentiation, and endocytosis. The findings from Rinl-KO mouse models have provided insights into its role in T cell-related immune regulation, highlighting its potential importance in immunological research and related disease areas.
References:
1. Kajiho, Hiroaki, Fukushima, Shinichi, Kontani, Kenji, Katada, Toshiaki. 2012. RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin. In PloS one, 7, e30575. doi:10.1371/journal.pone.0030575. https://pubmed.ncbi.nlm.nih.gov/22291991/
2. Sandner, Lisa, Alteneder, Marlis, Rica, Ramona, Herbst, Ruth, Boucheron, Nicole. 2023. The guanine nucleotide exchange factor Rin-like controls Tfh cell differentiation via CD28 signaling. In The Journal of experimental medicine, 220, . doi:10.1084/jem.20221466. https://pubmed.ncbi.nlm.nih.gov/37703004/
3. Woller, Barbara, Luiskandl, Susan, Popovic, Milica, Rehmann, Holger, Herbst, Ruth. 2011. Rin-like, a novel regulator of endocytosis, acts as guanine nucleotide exchange factor for Rab5a and Rab22. In Biochimica et biophysica acta, 1813, 1198-210. doi:10.1016/j.bbamcr.2011.03.005. https://pubmed.ncbi.nlm.nih.gov/21419809/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen