C57BL/6JCya-Rinlem1flox/Cya
Common Name
Rinl-flox
Product ID
S-CKO-10439
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-320435-Rinl-B6J-VA
Status
When using this mouse strain in a publication, please cite “Rinl-flox Mouse (Catalog S-CKO-10439) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Rinl-flox
Strain ID
CKOCMP-320435-Rinl-B6J-VA
Gene Name
Product ID
S-CKO-10439
Gene Alias
5830482F20Rik, 9930116N10Rik
Background
C57BL/6JCya
Gene Full Name
Ras and Rab interactor-like
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 7 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000059857
NCBI Transcript ID
NM_177158
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Overview of Gene Research
Rinl, also known as Ras and Rab interactor (or Ras interaction/interference)-like, is a guanine nucleotide exchange factor (GEF) for the Rab5 subfamily proteins. It contains a conserved VPS9 domain crucial for GEF action and is involved in membrane trafficking. Rinl is implicated in the EphA8-degradation pathway via its interaction with odin and also plays a role in regulating Tfh cell differentiation by controlling CD28 internalization and signaling. It is also associated with endocytotic processes regulated by Rab5a and Rab22 [1,2,3].
In mice, loss of Rinl leads to an increase of Tfh cells in aging, upon in vivo immunization, and acute LCMV Armstrong infection, indicating its role as a negative regulator of Tfh generation. This reveals its significance in shaping CD4+ T cell activation and differentiation [2].
In conclusion, Rinl is a key GEF that is involved in multiple biological processes, including protein degradation pathways, Tfh cell differentiation, and endocytosis. The findings from Rinl-KO mouse models have provided insights into its role in T cell-related immune regulation, highlighting its potential importance in immunological research and related disease areas.
References：
1. Kajiho, Hiroaki, Fukushima, Shinichi, Kontani, Kenji, Katada, Toshiaki. 2012. RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin. In PloS one, 7, e30575. doi:10.1371/journal.pone.0030575. https://pubmed.ncbi.nlm.nih.gov/22291991/
2. Sandner, Lisa, Alteneder, Marlis, Rica, Ramona, Herbst, Ruth, Boucheron, Nicole. 2023. The guanine nucleotide exchange factor Rin-like controls Tfh cell differentiation via CD28 signaling. In The Journal of experimental medicine, 220, . doi:10.1084/jem.20221466. https://pubmed.ncbi.nlm.nih.gov/37703004/
3. Woller, Barbara, Luiskandl, Susan, Popovic, Milica, Rehmann, Holger, Herbst, Ruth. 2011. Rin-like, a novel regulator of endocytosis, acts as guanine nucleotide exchange factor for Rab5a and Rab22. In Biochimica et biophysica acta, 1813, 1198-210. doi:10.1016/j.bbamcr.2011.03.005. https://pubmed.ncbi.nlm.nih.gov/21419809/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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