C57BL/6NCya-Vrtnem1flox/Cya
Common Name:
Vrtn-flox
Product ID:
S-CKO-11233
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vrtn-flox
Strain ID
CKOCMP-432677-Vrtn-B6N-VA
Gene Name
Product ID
S-CKO-11233
Gene Alias
7420416P09Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Vrtnem1flox/Cya mice (Catalog S-CKO-11233) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167227
NCBI RefSeq
NM_001168588
Target Region
Exon 3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Vrtn, or vertnin, is a gene with significant roles in mammalian development. It is a novel DNA-binding transcription factor that localizes in the nucleus, binds to DNA across the genome, and regulates the transcription of genes containing its binding motifs. Vrtn is essential for the development of thoracic vertebrae and is involved in somitogenesis. It also modulates somite segmentation via the Notch signaling pathway [1].
In Vrtn-null embryos, there are somitogenesis defects, including failure of axial rotation and fewer somites at the thoracic somite stage. Half of Vrtn heterozygous mice show abnormal spinal development with fewer thoracic vertebrae and ribs compared to wild-type littermates. These findings from mouse models reveal the crucial role of Vrtn in thoracic vertebrae development [1].
In conclusion, Vrtn is essential for the development of thoracic vertebrae in mammals. The gene knockout and heterozygous mouse models have provided valuable insights into its function in vertebral development. Understanding Vrtn can potentially contribute to improving pork production by selecting alleles that increase the number of thoracic vertebrae and ribs, as well as having implications for understanding the genetic basis of vertebral number in other animals like sheep [1,2,3].
References:
1. Duan, Yanyu, Zhang, Hui, Zhang, Zhen, Ren, Jun, Huang, Lusheng. 2018. VRTN is Required for the Development of Thoracic Vertebrae in Mammals. In International journal of biological sciences, 14, 667-681. doi:10.7150/ijbs.23815. https://pubmed.ncbi.nlm.nih.gov/29904281/
2. Yang, Jie, Huang, Lusheng, Yang, Ming, Gao, Jun, Ren, Jun. 2016. Possible introgression of the VRTN mutation increasing vertebral number, carcass length and teat number from Chinese pigs into European pigs. In Scientific reports, 6, 19240. doi:10.1038/srep19240. https://pubmed.ncbi.nlm.nih.gov/26781738/
3. Li, Cunyuan, Li, Ming, Li, Xiaoyue, Jiang, Yu, Hu, Shengwei. 2019. Whole-Genome Resequencing Reveals Loci Associated With Thoracic Vertebrae Number in Sheep. In Frontiers in genetics, 10, 674. doi:10.3389/fgene.2019.00674. https://pubmed.ncbi.nlm.nih.gov/31379930/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen