C57BL/6JCya-Nfat5em1flox/Cya
Common Name:
Nfat5-flox
Product ID:
S-CKO-11761
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nfat5-flox
Strain ID
CKOCMP-54446-Nfat5-B6J-VA
Gene Name
Product ID
S-CKO-11761
Gene Alias
B130038B15Rik; CAG-8; CAG80; NFATL1; OREBP; TonEBP; mKIAA0827; nfatz
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nfat5em1flox/Cya mice (Catalog S-CKO-11761) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169453
NCBI RefSeq
NM_018823
Target Region
Exon 6
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Nfat5, also known as tonicity-responsive enhancer-binding protein, is a member of the Rel family. It was initially identified in renal medullary cells, where it regulates the expression of osmoprotective-related genes during the osmotic response. It is also sensitive to various stress signals like heat shock, infection, etc. NFAT5-mediated signaling pathways play crucial roles in maintaining cellular homeostasis and are involved in numerous biological processes [2,5]. Genetic models such as gene knockout (KO) and conditional knockout (CKO) mouse models are valuable for studying its functions.
In glioblastoma, lysine methylation of NFAT5 at K668 promotes its activation, leading to upregulation of MGMT and unfavorable response to temozolomide therapy. Inhibition of this methylation improves the efficacy of temozolomide in xenograft models [1].
In neuropathic pain, NFAT5 selectively induces astrocyte swelling in the spinal dorsal horn by regulating aquaporin-4 expression, and its inhibition alleviates neuropathic pain [3].
In the tumor microenvironment, NFAT5 promotes CD8+ T cell exhaustion in a tumor-selective manner. Deletion of NFAT5 in CD8+ T cells improves tumor control [4].
In pancreatic cancer, NFAT5 governs cellular plasticity-driven resistance to KRAS-targeted therapy. Inhibiting NFAT5 attenuates pancreatitis-induced resistance to KRAS inhibition and extends mouse survival [6].
In conclusion, NFAT5 is a key regulator in maintaining cellular homeostasis, with its functions extending to multiple disease areas. Findings from KO/CKO mouse models have revealed its significance in glioblastoma treatment response, neuropathic pain, tumor-induced T cell exhaustion, and pancreatic cancer resistance to therapy, highlighting its potential as a therapeutic target.
References:
1. Li, Yatian, Gao, Zhenyue, Wang, Yuhong, Li, Min, Ren, Yu. 2023. Lysine methylation promotes NFAT5 activation and determines temozolomide efficacy in glioblastoma. In Nature communications, 14, 4062. doi:10.1038/s41467-023-39845-z. https://pubmed.ncbi.nlm.nih.gov/37429858/
2. Yang, Xing-Liang, Wang, Xin, Peng, Bi-Wen. 2018. NFAT5 Has a Job in the Brain. In Developmental neuroscience, 40, 289-300. doi:10.1159/000493789. https://pubmed.ncbi.nlm.nih.gov/30391952/
3. He, Liqiong, Ma, Shengyun, Ding, Zijin, Guo, Qulian, Huang, Changsheng. 2024. Inhibition of NFAT5-Dependent Astrocyte Swelling Alleviates Neuropathic Pain. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2302916. doi:10.1002/advs.202302916. https://pubmed.ncbi.nlm.nih.gov/38195869/
4. Tillé, Laure, Cropp, Daniela, Charmoy, Mélanie, Held, Werner, Verdeil, Grégory. 2023. Activation of the transcription factor NFAT5 in the tumor microenvironment enforces CD8+ T cell exhaustion. In Nature immunology, 24, 1645-1653. doi:10.1038/s41590-023-01614-x. https://pubmed.ncbi.nlm.nih.gov/37709986/
5. Zhao, Guangze, Aghakeshmiri, Sana, Chen, Yankuan T, Yip, Fione, Yang, Decheng. 2021. NFAT5-Mediated Signalling Pathways in Viral Infection and Cardiovascular Dysfunction. In International journal of molecular sciences, 22, . doi:10.3390/ijms22094872. https://pubmed.ncbi.nlm.nih.gov/34064510/
6. Deng, Daiyong, Begum, Habeebunnisa, Liu, Tong, Soteropoulos, Patricia, Hou, Pingping. 2024. NFAT5 governs cellular plasticity-driven resistance to KRAS-targeted therapy in pancreatic cancer. In The Journal of experimental medicine, 221, . doi:10.1084/jem.20240766. https://pubmed.ncbi.nlm.nih.gov/39432061/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen