C57BL/6JCya-Mtch2em1flox/Cya
Common Name:
Mtch2-flox
Product ID:
S-CKO-12091
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mtch2-flox
Strain ID
CKOCMP-56428-Mtch2-B6J-VA
Gene Name
Product ID
S-CKO-12091
Gene Alias
2310034D24Rik; 4930539J07Rik; HSPC032
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mtch2em1flox/Cya mice (Catalog S-CKO-12091) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000136872
NCBI RefSeq
NM_019758
Target Region
Exon 6~8
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
MTCH2, short for mitochondrial carrier homolog 2, is a member of the solute carrier 25 family located on the outer mitochondrial membrane. It functions as a mitochondrial outer membrane protein insertase, facilitating the insertion of various types of proteins into the membrane [1]. MTCH2 is also involved in multiple pathways such as mitochondrial apoptosis, fusion/fission, metabolic shift between glycolysis and oxidative phosphorylation, and epithelial-mesenchymal transition, playing a crucial role in biological processes like embryonic development, reproduction, and maintaining lipid and energy homeostasis [2,3,5]. Genetic models, including KO/CKO mouse models, have been valuable for studying its functions.
In mice, adipose-specific MTCH2 depletion protects against high-fat-diet-induced obesity and metabolic disorders. MTCH2 deficiency promotes energy expenditure by stimulating thermogenesis in brown adipose tissue and browning of subcutaneous white adipose tissue, revealing its role as a negative regulator of energy homeostasis [3]. Knocking down Mtch in Drosophila heart tubes leads to a dilated and poorly functioning heart tube, reduced adiposity, and shortened lifespan, indicating its importance in heart function and metabolism [4]. In addition, MTCH2 knockdown reduces lipid accumulation in adipocyte-like cells in vitro and in C. elegans and mice in vivo, while its overexpression increases fat accumulation, highlighting its role in lipid homeostasis [5].
In conclusion, MTCH2 is essential for multiple biological functions, especially in regulating mitochondrial function, energy and lipid homeostasis. Studies using KO/CKO mouse models and other genetic models have significantly enhanced our understanding of its roles in obesity, heart failure, and other related diseases, providing potential therapeutic targets for these conditions.
References:
1. Guna, Alina, Stevens, Taylor A, Inglis, Alison J, Weissman, Jonathan S, Voorhees, Rebecca M. 2022. MTCH2 is a mitochondrial outer membrane protein insertase. In Science (New York, N.Y.), 378, 317-322. doi:10.1126/science.add1856. https://pubmed.ncbi.nlm.nih.gov/36264797/
2. Peng, Xiaoqing, Yang, Yuanyuan, Hou, Ruirui, Yin, Zongzhi, Cao, Yunxia. 2024. MTCH2 in Metabolic Diseases, Neurodegenerative Diseases, Cancers, Embryonic Development and Reproduction. In Drug design, development and therapy, 18, 2203-2213. doi:10.2147/DDDT.S460448. https://pubmed.ncbi.nlm.nih.gov/38882047/
3. Zhao, Xin-Yuan, Zhao, Ben-Chi, Li, Hui-Lin, Neely, G Gregory, Wang, Qiao-Ping. 2025. MTCH2 Suppresses Thermogenesis by Regulating Autophagy in Adipose Tissue. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2416598. doi:10.1002/advs.202416598. https://pubmed.ncbi.nlm.nih.gov/40051328/
4. Fischer, Julie A, Monroe, Tanner O, Pesce, Lorenzo L, Puckelwartz, Megan J, McNally, Elizabeth M. . Opposing effects of genetic variation in MTCH2 for obesity versus heart failure. In Human molecular genetics, 32, 15-29. doi:10.1093/hmg/ddac176. https://pubmed.ncbi.nlm.nih.gov/35904451/
5. Rottiers, Veerle, Francisco, Adam, Platov, Michael, Gross, Atan, Libert, Sergiy. 2017. MTCH2 is a conserved regulator of lipid homeostasis. In Obesity (Silver Spring, Md.), 25, 616-625. doi:10.1002/oby.21751. https://pubmed.ncbi.nlm.nih.gov/28127879/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen