C57BL/6JCya-Tbataem1flox/Cya
Common Name:
Tbata-flox
Product ID:
S-CKO-12839
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tbata-flox
Strain ID
CKOCMP-65971-Tbata-B6J-VA
Gene Name
Product ID
S-CKO-12839
Gene Alias
1700021K02Rik; Spatial; Titest
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tbataem1flox/Cya mice (Catalog S-CKO-12839) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035894
NCBI RefSeq
NM_001017433.2
Target Region
Exon 4~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Tbata, also known as SPATIAL, is a gene with diverse functions. It plays a crucial role in regulating thymic epithelial cell (TEC) proliferation and thymus size by interacting with the enzyme Uba3 and inhibiting the Nedd8 pathway [1]. It is also involved in processes such as neurite outgrowth and dendrite patterning in hippocampal neurons, and may be associated with the enteric nervous system development as well as host resistance to the parasite Ichthyophthirius multifiliis [2,3,4].
In aged Tbata-deficient mice, thymi are larger and contain more dividing TECs compared to wild-type littermate controls. Additionally, thymic reconstitution after bone marrow transplantation occurs more rapidly in Rag2(-/-)Tbata(-/-) mice than in Rag2(-/-)Tbata(+/+) littermate controls. These findings from gene knockout mouse models suggest that Tbata modulates thymus function by regulating stromal cell proliferation via the Nedd8 pathway [1].
In conclusion, Tbata is essential for regulating thymus function through its impact on TEC proliferation. The gene knockout mouse models have provided valuable insights into the role of Tbata in thymus-related processes, which may have implications for understanding immune-related diseases and conditions associated with thymus function.
References：
1. Flomerfelt, Francis A, El Kassar, Nahed, Gurunathan, Chandra, Schwartz, Ronald H, Gress, Ronald E. 2010. Tbata modulates thymic stromal cell proliferation and thymus function. In The Journal of experimental medicine, 207, 2521-32. doi:10.1084/jem.20092759. https://pubmed.ncbi.nlm.nih.gov/20937703/
2. Yammine, Miriam, Saade, Murielle, Chauvet, Sophie, Nguyen, Catherine. 2013. Spatial gene's (Tbata) implication in neurite outgrowth and dendrite patterning in hippocampal neurons. In Molecular and cellular neurosciences, 59, 1-9. doi:10.1016/j.mcn.2013.12.007. https://pubmed.ncbi.nlm.nih.gov/24361585/
3. Gui, Hongsheng, Schriemer, Duco, Cheng, William W, Garcia-Barceló, Maria-Mercè, Hofstra, Robert M W. 2017. Whole exome sequencing coupled with unbiased functional analysis reveals new Hirschsprung disease genes. In Genome biology, 18, 48. doi:10.1186/s13059-017-1174-6. https://pubmed.ncbi.nlm.nih.gov/28274275/
4. Lira, Lieschen V G, Mastrochirico-Filho, Vito A, Mendes, Natalia J, Yáñez, José M, Hashimoto, Diogo T. 2022. Genome-wide association study of host resistance to the ectoparasite Ichthyophthirius multifiliis in the Amazon fish Colossoma macropomum. In Molecular biology reports, 50, 599-607. doi:10.1007/s11033-022-08062-0. https://pubmed.ncbi.nlm.nih.gov/36367660/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen