C57BL/6JCya-Dynlrb1em1flox/Cya
Common Name:
Dynlrb1-flox
Product ID:
S-CKO-13483
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dynlrb1-flox
Strain ID
CKOCMP-67068-Dynlrb1-B6J-VA
Gene Name
Product ID
S-CKO-13483
Gene Alias
2010012N15Rik; 2010320M17Rik; 9430076K19Rik; DNLC2A; Dncl2a; km23-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dynlrb1em1flox/Cya mice (Catalog S-CKO-13483) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000150602
NCBI RefSeq
NM_001291108
Target Region
Exon 3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Dynlrb1, also known as Dynein Light Chain Roadblock-Type 1, is an essential subunit of the dynein complex. It plays a crucial role in dynein-mediated transport, which is vital for the survival and function of highly polarized cells like neurons. Dynlrb1 is involved in multiple signaling pathways, such as the β-catenin subcellular localization pathway, and is associated with the axonal transport of lysosomes and retrograde signaling endosomes [1].
Homozygous Dynlrb1 null mice are not viable and die during early embryonic development [1]. Heterozygous or adult knockdown animals display reduced neuronal growth, and selective depletion of Dynlrb1 in proprioceptive neurons compromises their survival [1]. Conditional depletion of Dynlrb1 in sensory neurons causes deficits in several signaling pathways and severe impairment in axonal transport [1]. In addition, in male meiosis, the ubiquitously expressed Dynlrb1 maintains spindle bipolarity by targeting NuMA and suppressing centriole overduplication, while a related protein Dynlrb2 has a similar role specifically in male meiotic spindle formation [2].
In conclusion, Dynlrb1 is essential for general dynein-mediated transport and neuronal survival. Studies using Dynlrb1 knockout mouse models have revealed its critical role in early embryonic development and neuronal functions. Given dynein's key functions in neuronal physiology, Dynlrb1 could potentially play a prominent role in the etiology of human neurodegenerative diseases [1].
References:
1. Terenzio, Marco, Di Pizio, Agostina, Rishal, Ida, Fisher, Elizabeth M C, Fainzilber, Mike. 2020. DYNLRB1 is essential for dynein mediated transport and neuronal survival. In Neurobiology of disease, 140, 104816. doi:10.1016/j.nbd.2020.104816. https://pubmed.ncbi.nlm.nih.gov/32088381/
2. He, Shuwen, Gillies, John P, Zang, Juliana L, DeSantis, Morgan E, Shibuya, Hiroki. 2023. Distinct dynein complexes defined by DYNLRB1 and DYNLRB2 regulate mitotic and male meiotic spindle bipolarity. In Nature communications, 14, 1715. doi:10.1038/s41467-023-37370-7. https://pubmed.ncbi.nlm.nih.gov/36973253/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen