C57BL/6JCya-Pacrgem1flox/Cya
Common Name:
Pacrg-flox
Product ID:
S-CKO-14488
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Pacrg-flox
Strain ID
CKOCMP-69310-Pacrg-B6J-VA
Gene Name
Product ID
S-CKO-14488
Gene Alias
1700008H23Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pacrgem1flox/Cya mice (Catalog S-CKO-14488) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041463
NCBI RefSeq
NM_027032
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Pacrg, short for Parkin co-regulated gene, shares a bidirectional promoter with the Parkinson's disease-associated gene Parkin. It is involved in multiple biological processes. In sperm development, it forms a complex with MEIG1 in the manchette, which is crucial for sperm flagellum assembly [1,3,5]. In cilia, it is part of the inner junction of axonemal doublet microtubules and regulates ciliary motility [2]. It also has roles in cellular signaling, influencing gustatory plasticity and longevity in Caenorhabditis elegans [4].
Gene-knockout (KO) mouse models have been instrumental in understanding Pacrg's function. In male germ-cell-specific DNALI1-deficient mice, the localization of Pacrg within the manchette is affected, leading to abnormal sperm morphology and male infertility, highlighting its importance in sperm differentiation [1]. In Pacrg-deficient mice, MEIG1 no longer localizes to the manchette, and the transport of cargos like SPAG16L to build the sperm flagella is disrupted, emphasizing its role in sperm flagella formation [5].
In conclusion, Pacrg plays essential roles in sperm development, ciliary motility, and cellular signaling. The use of KO mouse models has revealed its significance in these processes, especially in relation to male infertility. Understanding Pacrg's functions provides insights into the mechanisms underlying sperm-related disorders and ciliary-associated diseases.
References:
1. Yap, Yi Tian, Li, Wei, Huang, Qian, Toure, Aminata, Zhang, Zhibing. 2023. DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice. In eLife, 12, . doi:10.7554/eLife.79620. https://pubmed.ncbi.nlm.nih.gov/37083624/
2. Dymek, Erin E, Lin, Jianfeng, Fu, Gang, Nicastro, Daniela, Smith, Elizabeth F. 2019. PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility. In Molecular biology of the cell, 30, 1805-1816. doi:10.1091/mbc.E19-01-0063. https://pubmed.ncbi.nlm.nih.gov/31116684/
3. Hasse, Timothy, Zhang, Zhibing, Huang, Yu-Ming M. 2022. Molecular dynamics study reveals key disruptors of MEIG1-PACRG interaction. In Proteins, 91, 555-566. doi:10.1002/prot.26449. https://pubmed.ncbi.nlm.nih.gov/36444670/
4. Loucks, Catrina M, Bialas, Nathan J, Dekkers, Martijn P J, Jansen, Gert, Leroux, Michel R. 2016. PACRG, a protein linked to ciliary motility, mediates cellular signaling. In Molecular biology of the cell, 27, 2133-44. doi:10.1091/mbc.E15-07-0490. https://pubmed.ncbi.nlm.nih.gov/27193298/
5. Li, Wei, Tang, Waixing, Teves, Maria E, Strauss, Jerome F, Zhang, Zhibing. . A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella. In Development (Cambridge, England), 142, 921-30. doi:10.1242/dev.119834. https://pubmed.ncbi.nlm.nih.gov/25715396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen