C57BL/6JCya-Apmapem1flox/Cya
Common Name:
Apmap-flox
Product ID:
S-CKO-15237
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Apmap-flox
Strain ID
CKOCMP-71881-Apmap-B6J-VA
Gene Name
Product ID
S-CKO-15237
Gene Alias
2310001A20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Apmapem1flox/Cya mice (Catalog S-CKO-15237) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046399
NCBI RefSeq
NM_027977
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Apmap, or adipocyte plasma membrane-associated protein, is a single transmembrane arylesterase and glycosyl type II transmembrane protein. It is mainly distributed in the plasma membrane and endoplasmic reticulum of adipocytes and plays a crucial role in adipogenesis [2,3]. Apmap has been implicated in lipid transport, and is associated with pathways such as the Wnt/β-catenin pathway [3]. It also has potential importance in various biological processes like cancer cell phagocytosis, meat quality determination, and viral infection [1,2,5]. Genetic models, especially knockout models, are valuable for studying its functions.
In Apmap-knockout mice, the absence of the full-length APMAP protein, which is the only isoform expressed in humans, leads to an improved metabolic phenotype upon diet-induced obesity. This includes enhanced insulin sensitivity, preserved glucose tolerance, increased respiratory exchange ratio, decreased inflammatory marker gene expression, and reduced adipocyte size. APMAP was found to interact with extracellular collagen cross-linking matrix proteins lysyl oxidase-like 1 and 3, and their expression was decreased in Apmap-knockout mice, indicating decreased fibrotic potential in epididymal white adipose tissue [6]. In cancer research, loss of Apmap in cancer cells synergizes with tumour antigen-targeting monoclonal antibodies and/or CD47-blocking monoclonal antibodies to drive increased phagocytosis across various cancer cell types, and also inhibits tumour growth in mice [1]. In cervical cancer, high expression of Apmap is associated with poor prognosis, and knockdown of Apmap in vitro significantly inhibits the migration ability of cervical cancer cells by suppressing the activation of the Wnt/β-catenin pathway [3].
In conclusion, Apmap is a multi-functional protein involved in adipogenesis, lipid and lipoprotein homeostasis, and has implications in diseases such as obesity-related metabolic disorders and cancers [2,4,6]. Studies using Apmap knockout mouse models have revealed its role in modulating metabolic phenotypes and cancer cell susceptibility to phagocytosis, providing insights into potential therapeutic targets for these disease areas.
References:
1. Kamber, Roarke A, Nishiga, Yoko, Morton, Bhek, Sage, Julien, Bassik, Michael C. 2021. Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis. In Nature, 597, 549-554. doi:10.1038/s41586-021-03879-4. https://pubmed.ncbi.nlm.nih.gov/34497417/
2. Luo, Gang, Mu, Jinzhan, Wang, Shuhui, Dong, Xianggui, Ren, Zhanjun. 2022. Association of blood APMAP content and meat quality trait in Rex rabbits. In Animal biotechnology, 34, 974-979. doi:10.1080/10495398.2021.2007117. https://pubmed.ncbi.nlm.nih.gov/35001846/
3. Zhu, Xiuting, Xiang, Zijin, Zou, Lingxiao, Peng, Xiangdong, Xu, Dabao. 2021. APMAP Promotes Epithelial-Mesenchymal Transition and Metastasis of Cervical Cancer Cells by Activating the Wnt/β-catenin Pathway. In Journal of Cancer, 12, 6265-6273. doi:10.7150/jca.59595. https://pubmed.ncbi.nlm.nih.gov/34539899/
4. Paul, Blessy, Merta, Holly, Ugrankar-Banerjee, Rupali, Farber, Steven A, Henne, W Mike. 2024. Paraoxonase-like APMAP maintains endoplasmic reticulum-associated lipid and lipoprotein homeostasis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.26.577049. https://pubmed.ncbi.nlm.nih.gov/38328083/
5. Haley, Sheila A, O'Hara, Bethany A, Atwood, Walter J. 2020. Adipocyte Plasma Membrane Protein (APMAP) promotes JC Virus (JCPyV) infection in human glial cells. In Virology, 548, 17-24. doi:10.1016/j.virol.2020.06.002. https://pubmed.ncbi.nlm.nih.gov/32838939/
6. Pessentheiner, Ariane R, Huber, Katharina, Pelzmann, Helmut J, Bilban, Martin, Bogner-Strauss, Juliane G. 2017. APMAP interacts with lysyl oxidase-like proteins, and disruption of Apmap leads to beneficial visceral adipose tissue expansion. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31, 4088-4103. doi:10.1096/fj.201601337R. https://pubmed.ncbi.nlm.nih.gov/28559441/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen