C57BL/6JCya-Tgfbrap1em1flox/Cya
Common Name:
Tgfbrap1-flox
Product ID:
S-CKO-15631
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tgfbrap1-flox
Strain ID
CKOCMP-73122-Tgfbrap1-B6J-VA
Gene Name
Product ID
S-CKO-15631
Gene Alias
3110018K12Rik; Trap1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tgfbrap1em1flox/Cya mice (Catalog S-CKO-15631) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095014
NCBI RefSeq
NM_001013025
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Tgfbrap1, also known as TGF-β receptor-associated binding protein 1, plays crucial roles in multiple biological processes. It is involved in the TGF-β signaling pathway, which regulates various cellular functions such as cell growth, differentiation, apoptosis, and extracellular matrix production. Dysregulation of this pathway can lead to numerous diseases [1,5,7].
In pulmonary fibrosis, Chitinase 1 (CHIT1) enhances TGF-β1-stimulated fibrotic responses through inhibiting TGF-β1 induction of SMAD7. This effect is mediated by CHIT1's interaction with Tgfbrap1, highlighting Tgfbrap1's role in the pathogenesis of pulmonary fibrosis [1,7].
In goose follicular granulosa cells, knockdown of Tgfbrap1 mRNA level reduces apoptosis rate and enhances the secretion of E2 and P4, indicating its role in regulating follicular development [2].
Also, a variant at Tgfbrap1 (rs2241797) is significantly associated with type 2 diabetes mellitus and affects diabetes-related miRNA expression [3].
Additionally, the A variant of rs17687727 in Tgfbrap1 influences the susceptibility to antituberculosis drug-induced liver injury in the Han Chinese population [4].
In liver cancer, Tgfbrap1 stabilizes TGF-β receptor type 1 (TGFBR1), potentiating TGF-β signaling and promoting liver cancer stemness and drug resistance [5].
Piperine, which protects against acetaminophen-induced hepatotoxicity, may mediate its effects through regulating Tgfbrap1 [6].
Overexpression of Tgfbrap1, a CORVET-specific subunit, decreases the amount of assembled HOPS complex, affecting autophagosome-lysosome fusion and lysosomal hydrolase delivery [8].
In conclusion, Tgfbrap1 is essential in the TGF-β signaling pathway and is involved in various biological processes and disease conditions such as pulmonary fibrosis, follicular development, type 2 diabetes, antituberculosis drug-induced liver injury, liver cancer, hepatotoxicity response, and endosomal tethering complex regulation. The study of Tgfbrap1 through genetic models helps to understand its function in these diseases, potentially providing new insights for disease treatment and prevention.
References：
1. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2024. Retraction: Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 7, . doi:10.26508/lsa.202402987. https://pubmed.ncbi.nlm.nih.gov/39209538/
2. Wang, Zhixiu, Lu, Lu, Gu, Tiantian, Xu, Qi, Chen, Guohong. 2020. The effects of FAR1 and TGFBRAP1 on the proliferation and apoptosis of follicular granulosa cells in goose (Anser cygnoides). In Gene, 769, 145194. doi:10.1016/j.gene.2020.145194. https://pubmed.ncbi.nlm.nih.gov/33007376/
3. Yang, Song, Chen, Xiaotian, Yang, Mengyao, Liu, Chunlan, Shen, Chong. 2018. The variant at TGFBRAP1 is significantly associated with type 2 diabetes mellitus and affects diabetes-related miRNA expression. In Journal of cellular and molecular medicine, 23, 83-92. doi:10.1111/jcmm.13885. https://pubmed.ncbi.nlm.nih.gov/30461200/
4. Zhang, Jingwei, Zhao, Zhenzhen, Bai, Hao, Lyv, Mengyuan, Ying, Binwu. 2019. The Variant at TGFBRAP1 but Not TGFBR2 Is Associated with Antituberculosis Drug-Induced Liver Injury. In Evidence-based complementary and alternative medicine : eCAM, 2019, 1685128. doi:10.1155/2019/1685128. https://pubmed.ncbi.nlm.nih.gov/31534460/
5. Liu, Kewei, Tian, Fanxuan, Chen, Xu, Wang, Tao, Wang, Bin. 2024. Stabilization of TGF-β Receptor 1 by a Receptor-Associated Adaptor Dictates Feedback Activation of the TGF-β Signaling Pathway to Maintain Liver Cancer Stemness and Drug Resistance. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2402327. doi:10.1002/advs.202402327. https://pubmed.ncbi.nlm.nih.gov/38981014/
6. Morsy, M A, Younis, N S, El-Sheikh, A A K, El-Daly, M, Mohafez, O M. . Protective mechanisms of piperine against acetaminophen-induced hepatotoxicity may be mediated through TGFBRAP1. In European review for medical and pharmacological sciences, 24, 10169-10180. doi:10.26355/eurrev_202010_23237. https://pubmed.ncbi.nlm.nih.gov/33090425/
7. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2019. Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 2, . doi:10.26508/lsa.201900350. https://pubmed.ncbi.nlm.nih.gov/31085559/
8. Sőth, Ármin, Molnár, Márton, Lőrincz, Péter, Simon-Vecsei, Zsófia, Juhász, Gábor. 2024. CORVET-specific subunit levels determine the balance between HOPS/CORVET endosomal tethering complexes. In Scientific reports, 14, 10146. doi:10.1038/s41598-024-59775-0. https://pubmed.ncbi.nlm.nih.gov/38698024/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen