C57BL/6JCya-Tmed5em1flox/Cya
Common Name:
Tmed5-flox
Product ID:
S-CKO-15633
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tmed5-flox
Strain ID
CKOCMP-73130-Tmed5-B6J-VA
Gene Name
Product ID
S-CKO-15633
Gene Alias
3110020O18Rik; 4432412D15Rik; p24gamma2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed5em1flox/Cya mice (Catalog S-CKO-15633) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002837
NCBI RefSeq
NM_028876
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
TMED5, also known as transmembrane P24 trafficking protein 5, is involved in multiple biological processes. In mammals, it may be related to immune response, signal transduction, growth, and disease development [4]. Although its exact essential function and associated pathways are still being explored, studies suggest it could play a role in pathways like the mammalian target of rapamycin and transforming growth factor beta signaling pathways [1].
In hepatocellular carcinoma (HCC), TMED5 mRNA is highly expressed in HCC tissues compared to adjacent normal tissues. Silencing TMED5 in SMMC-7721 and Hep3B cells suppressed cell proliferation, migration, and invasion, while enhancing apoptosis, indicating its potential role in promoting HCC progression [1].
In gastric cancer, the TP73-AS1/miR-27b-3p/TMED5 axis was found to regulate the wnt/β-catenin pathway, with TMED5 up-regulated and promoting cancer cell proliferation, migration, and invasion [2].
In cervical cancer, a circ_0018289/miR-183-5p/TMED5 regulatory network was identified, where circ_0018289 induced TMED5 expression by competitively binding to miR-183-5p, and TMED5 promoted cancer development and angiogenesis [3].
In conclusion, TMED5 seems to play a role in promoting the progression of several cancers, including HCC, gastric cancer, and cervical cancer. These findings from cell-based functional studies provide insights into the role of TMED5 in disease conditions, suggesting that it could potentially be a therapeutic target for these cancers.
References：
1. Cheng, Xianyi, Deng, Xiulan, Zeng, Huiping, Li, Dezhi, Zheng, Wei V. . Silencing of TMED5 inhibits proliferation, migration and invasion, and enhances apoptosis of hepatocellular carcinoma cells. In Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 32, 677-688. doi:10.17219/acem/156673. https://pubmed.ncbi.nlm.nih.gov/36530030/
2. Bao, Chenhui, Guo, Lin. 2022. TP73-AS1 promotes gastric cancer proliferation and invasion by regulation miR-27b-3p/TMED5 axis. In Journal of Cancer, 13, 1324-1335. doi:10.7150/jca.66683. https://pubmed.ncbi.nlm.nih.gov/35281863/
3. Zou, Heng, Chen, Huijia, Liu, Shuaibin, Gan, Xiaoling. 2021. Identification of a novel circ_0018289/miR-183-5p/TMED5 regulatory network in cervical cancer development. In World journal of surgical oncology, 19, 246. doi:10.1186/s12957-021-02350-y. https://pubmed.ncbi.nlm.nih.gov/34404391/
4. Wang, Chunyang, Guo, Yu, Li, Haiyin, Chen, Ping. . [Analyzing the evolution of insect TMED gene and the expression pattern of silkworm TMED gene]. In Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 39, 4996-5013. doi:10.13345/j.cjb.230251. https://pubmed.ncbi.nlm.nih.gov/38147997/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen