C57BL/6JCya-Irak3em1flox/Cya
Common Name:
Irak3-flox
Product ID:
S-CKO-15795
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Irak3-flox
Strain ID
CKOCMP-73914-Irak3-B6J-VA
Gene Name
Product ID
S-CKO-15795
Gene Alias
4833428C18Rik; IRAK-M
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Irak3em1flox/Cya mice (Catalog S-CKO-15795) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020448
NCBI RefSeq
NM_028679
Target Region
Exon 5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Interleukin-1 receptor associated kinase 3 (IRAK3) is a cytoplasmic homeostatic mediator of inflammatory responses. It inhibits signalling cascades downstream of myddosome complexes related to toll-like receptors. IRAK3 contains a death domain, a pseudokinase domain with a guanylate cyclase centre, and a C-terminus domain associated with tumour necrosis factor receptor associated factor 6 (TRAF6) [1]. It is involved in the MyD88-TRAF6 pathway and is a key modulator in the human inflammatory pathway, also associated with endotoxin tolerance and sepsis [1,2,3,5,6].
In acute pancreatitis, Irak3-/-mice showed enhanced migration of CCR2 + monocytes into the pancreas and a pro-inflammatory type 1 immune response. In a mild model, this led to decreased pancreatic damage, while in a severe model, it drove a severe systemic inflammatory response syndrome (SIRS) and increased local and systemic damage. This indicates that IRAK3-mediated suppression of pro-inflammatory MyD88/IRAK signaling affects disease severity [4].
In breast cancer, silencing of circular RNA circ_IRAK3 induced apoptosis, curbed cell proliferation, migration, and invasion, and decreased tumor growth in vivo. circ_IRAK3 promoted breast cancer progression by modulating the miR-603/KIF2A axis [7].
In rheumatoid arthritis, CD14 + monocytes from patients with high disease activity had lower levels of IRAK3 expression compared to those with low/moderate activity [8].
In conclusion, IRAK3 is a crucial modulator of inflammatory responses. Its function is revealed through gene-knockout models in various disease conditions such as acute pancreatitis, breast cancer, and rheumatoid arthritis. These models help understand its role in disease development, providing potential targets for treatment.
References:
1. Freihat, L A, Wheeler, J I, Wong, A, Manallack, D T, Irving, H R. 2019. IRAK3 modulates downstream innate immune signalling through its guanylate cyclase activity. In Scientific reports, 9, 15468. doi:10.1038/s41598-019-51913-3. https://pubmed.ncbi.nlm.nih.gov/31664109/
2. Weng, Panwei, Lan, Mengjiao, Zhang, Hao, Xu, Anlong, Huang, Shengfeng. . Both IRAK3 and IRAK1 Activate the MyD88-TRAF6 Pathway in Zebrafish. In Journal of immunology (Baltimore, Md. : 1950), 213, 362-372. doi:10.4049/jimmunol.2400054. https://pubmed.ncbi.nlm.nih.gov/38847613/
3. Rowley, Ann, Brown, Brian S, Stofega, Mary, Rivkin, Alexey, Woller, Kevin R. 2022. Targeting IRAK3 for Degradation to Enhance IL-12 Pro-inflammatory Cytokine Production. In ACS chemical biology, 17, 1315-1320. doi:10.1021/acschembio.2c00037. https://pubmed.ncbi.nlm.nih.gov/35580266/
4. Thiel, Franziska G, Asgarbeik, Saeedeh, Glaubitz, Juliane, Weiss, Frank Ulrich, Sendler, Matthias. 2023. IRAK3-mediated suppression of pro-inflammatory MyD88/IRAK signaling affects disease severity in acute pancreatitis. In Scientific reports, 13, 10833. doi:10.1038/s41598-023-37930-3. https://pubmed.ncbi.nlm.nih.gov/37402858/
5. Nguyen, Trang Hong, Turek, Ilona, Meehan-Andrews, Terri, Zacharias, Anita, Irving, Helen. 2020. Analysis of interleukin-1 receptor associated kinase-3 (IRAK3) function in modulating expression of inflammatory markers in cell culture models: A systematic review and meta-analysis. In PloS one, 15, e0244570. doi:10.1371/journal.pone.0244570. https://pubmed.ncbi.nlm.nih.gov/33382782/
6. Nguyen, Trang H, Turek, Ilona, Meehan-Andrews, Terri, Zacharias, Anita, Irving, Helen R. 2022. A systematic review and meta-analyses of interleukin-1 receptor associated kinase 3 (IRAK3) action on inflammation in in vivo models for the study of sepsis. In PloS one, 17, e0263968. doi:10.1371/journal.pone.0263968. https://pubmed.ncbi.nlm.nih.gov/35167625/
7. Wang, Fang, Li, Jingruo, Li, Lin, Guo, Guangcheng, Gu, Yuanting. 2022. Circular RNA circ_IRAK3 contributes to tumor growth through upregulating KIF2A via adsorbing miR-603 in breast cancer. In Cancer cell international, 22, 81. doi:10.1186/s12935-022-02497-y. https://pubmed.ncbi.nlm.nih.gov/35164763/
8. Gomes da Silva, Isaura Isabelle Fonseca, Barbosa, Alexandre Domingues, Souto, Fabricio Oliveira, Souza, Paulo Roberto Eleuterio de, Sandrin-Garcia, Paula. 2021. MYD88, IRAK3 and Rheumatoid Arthritis pathogenesis: Analysis of differential gene expression in CD14 + monocytes and the inflammatory cytokine levels. In Immunobiology, 226, 152152. doi:10.1016/j.imbio.2021.152152. https://pubmed.ncbi.nlm.nih.gov/34735922/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen