C57BL/6JCya-Fabp12em1flox/Cya
Common Name:
Fabp12-flox
Product ID:
S-CKO-16240
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fabp12-flox
Strain ID
CKOCMP-75497-Fabp12-B6J-VA
Gene Name
Product ID
S-CKO-16240
Gene Alias
1700008G05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fabp12em1flox/Cya mice (Catalog S-CKO-16240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029043
NCBI RefSeq
NM_029310
Target Region
Exon 4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Fabp12, a member of the fatty acid-binding protein (FABP) family, has the same structure as other FABP genes and resides in a cluster with FABP4/5/8/9 within a 300,000 bp chromosomal region [2]. It is involved in reversibly binding intracellular hydrophobic ligands and trafficking them throughout cellular compartments, and is thought to play a role in lipid-related processes [4].
In prostate cancer, FABP12 is preferentially amplified and/or overexpressed in metastatic tumors compared to primary ones. It concurrently triggers metastatic phenotypes like epithelial-to-mesenchymal transition (EMT), leading to increased cell motility and invasion, and lipid bioenergetics such as increased fatty acid uptake, accumulation, and ATP production from fatty acid β-oxidation [1]. Additionally, the FABP12-Slug-Survivin pathway has been found to drive docetaxel resistance in prostate cancer cells, with high levels of Survivin associated with poor prognosis in PCa patients, and FABP12 status determining its prognostic significance [3].
In summary, Fabp12 plays crucial roles in lipid-related processes and is significantly involved in prostate cancer metastasis and chemoresistance. The findings from prostate cancer-related studies help to understand the role of Fabp12 in these disease conditions, potentially providing new avenues for targeted treatment strategies in prostate cancer [1,3].
References：
1. Liu, Rong-Zong, Choi, Won-Shik, Jain, Saket, Lemieux, Hélène, Godbout, Roseline. 2020. The FABP12/PPARγ pathway promotes metastatic transformation by inducing epithelial-to-mesenchymal transition and lipid-derived energy production in prostate cancer cells. In Molecular oncology, 14, 3100-3120. doi:10.1002/1878-0261.12818. https://pubmed.ncbi.nlm.nih.gov/33031638/
2. Liu, Rong-Zong, Li, Xiaodong, Godbout, Roseline. 2008. A novel fatty acid-binding protein (FABP) gene resulting from tandem gene duplication in mammals: transcription in rat retina and testis. In Genomics, 92, 436-45. doi:10.1016/j.ygeno.2008.08.003. https://pubmed.ncbi.nlm.nih.gov/18786628/
3. Liu, Rong-Zong, Garg, Mansi, Yang, Xiao-Hong, Godbout, Roseline. 2024. Docetaxel-Induced Cell Death Is Regulated by a Fatty Acid-Binding Protein 12-Slug-Survivin Pathway in Prostate Cancer Cells. In International journal of molecular sciences, 25, . doi:10.3390/ijms25179669. https://pubmed.ncbi.nlm.nih.gov/39273616/
4. Smathers, Rebecca L, Petersen, Dennis R. . The human fatty acid-binding protein family: evolutionary divergences and functions. In Human genomics, 5, 170-91. doi:. https://pubmed.ncbi.nlm.nih.gov/21504868/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen