C57BL/6JCya-Itprid2em1flox/Cya
Common Name
Itprid2-flox
Product ID
S-CKO-17532
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-70599-Itprid2-B6J-VC
When using this mouse strain in a publication, please cite “Itprid2-flox Mouse (Catalog S-CKO-17532) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Itprid2-flox
Strain ID
CKOCMP-70599-Itprid2-B6J-VC
Gene Name
Product ID
S-CKO-17532
Gene Alias
5730488C15Rik, CS-1, CS1, KRAP, SPAG13, Ssfa2, mKIAA1927
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000111785
NCBI RefSeq
NM_080558
Target Region
Exon 3~4
Size of Effective Region
~1.9 kb
Overview of Gene Research
Itprid2, also known as KRAS-induced actin-interacting protein (KRAP), is a cytoplasmic protein associated with filamentous-actin (F-actin). It plays a crucial role in regulating inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) activity. IP3Rs are channels that regulate the redistribution of Ca2+ from the endoplasmic reticulum (ER) to the cytosol and other organelles. Itprid2 is involved in the Ca2+ signaling pathway, which is essential for various biological processes such as cell survival, adaptation, and death [2,3].
Studies in Itprid2-deficient mice have shown that loss of Itprid2 abolishes cytosolic and mitochondrial Ca2+ signals evoked by stimulation of IP3Rs via endogenous receptors. It is located at ER-mitochondrial membrane contact sites (ERMCSs) populated by IP3R clusters, and its loss reduces the number of ERMCSs. This indicates that Itprid2 regulates Ca2+ transfer from IP3Rs to mitochondria by licensing IP3R activity and stabilizing ERMCSs [1]. Additionally, knockdown of Itprid2 in cultured HEK293 or MCF7 cells diminishes ATP-induced Ca2+ release, suggesting its role in the proper regulation of IP3R-mediated Ca2+ release [4].
In conclusion, Itprid2 is essential for regulating IP3R-mediated Ca2+ release and Ca2+ transfer between the ER and mitochondria. Research using Itprid2-deficient mouse models has provided valuable insights into its role in the Ca2+ signaling pathway, which may have implications for understanding diseases related to abnormal Ca2+ signaling, such as certain types of cancer [1,4].
References：
1. Atakpa-Adaji, Peace, Ivanova, Adelina, Kujawa, Karolina, Taylor, Colin W. 2024. KRAP regulates mitochondrial Ca2+ uptake by licensing IP3 receptor activity and stabilizing ER-mitochondrial junctions. In Journal of cell science, 137, . doi:10.1242/jcs.261728. https://pubmed.ncbi.nlm.nih.gov/38786982/
2. Fujimoto, Takahiro, Shirasawa, Senji. . KRAS-induced actin-interacting protein: a potent target for obesity, diabetes and cancer. In Anticancer research, 31, 2413-7. doi:. https://pubmed.ncbi.nlm.nih.gov/21873152/
3. Akl, Haidar, Bultynck, Geert. 2012. Altered Ca(2+) signaling in cancer cells: proto-oncogenes and tumor suppressors targeting IP3 receptors. In Biochimica et biophysica acta, 1835, 180-93. doi:10.1016/j.bbcan.2012.12.001. https://pubmed.ncbi.nlm.nih.gov/23232185/
4. Fujimoto, Takahiro, Machida, Takashi, Tsunoda, Toshiyuki, Kuroki, Masahide, Shirasawa, Senji. 2011. KRAS-induced actin-interacting protein regulates inositol 1,4,5-trisphosphate-receptor-mediated calcium release. In Biochemical and biophysical research communications, 408, 214-7. doi:10.1016/j.bbrc.2011.03.112. https://pubmed.ncbi.nlm.nih.gov/21457704/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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