C57BL/6JCya-Dckem1flox/Cya
Common Name:
Dck-flox
Product ID:
S-CKO-17832
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dck-flox
Strain ID
CKOCMP-13178-Dck-B6J-VB
Gene Name
Product ID
S-CKO-17832
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dckem1flox/Cya mice (Catalog S-CKO-17832) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031311
NCBI RefSeq
NM_007832
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Deoxycytidine kinase (dCK) is an enzyme in the nucleoside biosynthetic pathway, specifically in the nucleotide salvage pathway. It catalyzes the phosphorylation of deoxycytidine and other nucleoside analogs, a crucial step for their activation and incorporation into DNA, thus playing an important role in DNA synthesis and repair [1,2,3].
In cancer research, inhibition of dCK has shown potential. In BRCA2-deficient cancers, dCK inhibition is synthetic lethal, suggesting it could be a new target for these cancers, offering an alternative to PARP inhibitors [1]. In acute myeloid leukemia, DCK mutations were found in some patients relapsing after cytarabine-based therapy, contributing to cytarabine resistance, and overexpression of wild-type DCK restored cytarabine sensitivity in resistant cell lines [2]. Also, in gemcitabine-resistant human cancer cells, DCK was frequently inactivated, and restoring DCK expression could reverse gemcitabine resistance [3].
In conclusion, dCK is essential for nucleotide salvage and DNA synthesis. Its role in cancer drug resistance and as a potential synthetic lethal target in BRCA2-deficient cancers makes it an important gene in oncology research. Understanding dCK's function through various genetic models helps in developing more effective cancer treatment strategies.
References:
1. Guantay, Laura, Garro, Cintia, Siri, Sebastián, Gottifredi, Vanesa, Soria, Gastón. 2023. Deoxycytidine kinase (dCK) inhibition is synthetic lethal with BRCA2 deficiency. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 67, 100932. doi:10.1016/j.drup.2023.100932. https://pubmed.ncbi.nlm.nih.gov/36706533/
2. Wu, Biao, Mao, Zhengwei Jenny, Wang, Zhi, Gale, Robert Peter, Yin, Bin. 2021. Deoxycytidine Kinase (DCK) Mutations in Human Acute Myeloid Leukemia Resistant to Cytarabine. In Acta haematologica, 144, 534-541. doi:10.1159/000513696. https://pubmed.ncbi.nlm.nih.gov/33626530/
3. Saiki, Yuriko, Yoshino, Yuki, Fujimura, Hiroko, Unno, Michiaki, Horii, Akira. 2012. DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells. In Biochemical and biophysical research communications, 421, 98-104. doi:10.1016/j.bbrc.2012.03.122. https://pubmed.ncbi.nlm.nih.gov/22490663/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen