C57BL/6JCya-Rnf25em1flox/Cya
Common Name:
Rnf25-flox
Product ID:
S-CKO-18098
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rnf25-flox
Strain ID
CKOCMP-57751-Rnf25-B6J-VB
Gene Name
Product ID
S-CKO-18098
Gene Alias
0610009H16Rik; AO7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rnf25em1flox/Cya mice (Catalog S-CKO-18098) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027357
NCBI RefSeq
NM_021313
Target Region
Exon 2~5
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Rnf25, also known as AO7, is an E3 ubiquitin ligase that plays diverse roles in multiple biological processes and disease-related pathways. It is involved in pathways such as Wnt signaling, ribosome-associated quality control, and is relevant in diseases like cancer and potentially Parkinson's disease. Genetic models, including gene knockout and conditional knockout mouse models, could be valuable in further elucidating its functions [4,2,7,6].
In cancer, loss-of-function studies have shown significant implications. Repression of Rnf25 induces E-cadherin protein expression and inhibits hepatocellular carcinoma (HCC) metastasis, indicating its role in promoting HCC metastasis through E-cadherin degradation [1]. In EGFR-mutant non-small cell lung cancer (NSCLC) cells, depletion of Rnf25 sensitizes cells to gefitinib treatment, suggesting it is essential for gefitinib resistance by mediating cross-talk between NF-κB and ERK pathways [3]. In colorectal cancer liver metastasis, circSATB1 promotes FKBP8 degradation via RNF25-mediated ubiquitination, enhancing metastatic ability [5].
In conclusion, Rnf25 is a key E3 ubiquitin ligase with essential functions in multiple biological processes, especially in cancer-related pathways. Model-based research, particularly gene knockout studies in relevant cancer models, has revealed its importance in promoting cancer metastasis and drug resistance, providing potential therapeutic targets for these diseases.
References：
1. Huang, Zhao, Zhou, Li, Duan, Jiufei, Zhang, Wei, Huang, Canhua. 2024. Oxidative Stress Promotes Liver Cancer Metastasis via RNF25-Mediated E-Cadherin Protein Degradation. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2306929. doi:10.1002/advs.202306929. https://pubmed.ncbi.nlm.nih.gov/38286671/
2. Oltion, Keely, Carelli, Jordan D, Yang, Tangpo, Kampmann, Martin, Taunton, Jack. 2023. An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes. In Cell, 186, 346-362.e17. doi:10.1016/j.cell.2022.12.025. https://pubmed.ncbi.nlm.nih.gov/36638793/
3. Cho, Jung Hee, You, Yeon-Mi, Yeom, Y I, Kim, Jung-Ae, Park, Kyung Chan. 2018. RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-κB-mediated ERK reactivation. In Cell death & disease, 9, 587. doi:10.1038/s41419-018-0651-5. https://pubmed.ncbi.nlm.nih.gov/29789542/
4. Gao, Rui, Ma, Lin-Qiang, Du, Xiaogang, Cheng, Zhi, Huang, Huizhe. . Rnf25/AO7 positively regulates wnt signaling via disrupting Nkd1-Axin inhibitory complex independent of its ubiquitin ligase activity. In Oncotarget, 7, 23850-9. doi:10.18632/oncotarget.8126. https://pubmed.ncbi.nlm.nih.gov/27007149/
5. Zhang, Chuan, Tian, Chuanxin, Zhu, Renzhong, Zhang, Yue, Sun, Yueming. 2025. CircSATB1 Promotes Colorectal Cancer Liver Metastasis through Facilitating FKBP8 Degradation via RNF25-Mediated Ubiquitination. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2406962. doi:10.1002/advs.202406962. https://pubmed.ncbi.nlm.nih.gov/39921520/
6. Wettergren, Erika Elgstrand, Gussing, Fredrik, Quintino, Luis, Lundberg, Cecilia. 2012. Novel disease-specific promoters for use in gene therapy for Parkinson's disease. In Neuroscience letters, 530, 29-34. doi:10.1016/j.neulet.2012.09.059. https://pubmed.ncbi.nlm.nih.gov/23063686/
7. Gurzeler, Lukas-Adrian, Link, Marion, Ibig, Yvonne, Mühlemann, Oliver, Reinhardt, Jürgen. 2023. Drug-induced eRF1 degradation promotes readthrough and reveals a new branch of ribosome quality control. In Cell reports, 42, 113056. doi:10.1016/j.celrep.2023.113056. https://pubmed.ncbi.nlm.nih.gov/37651229/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen