C57BL/6JCya-Alas1em1flox/Cya
Common Name:
Alas1-flox
Product ID:
S-CKO-18255
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Alas1-flox
Strain ID
CKOCMP-11655-Alas1-B6J-VB
Gene Name
Product ID
S-CKO-18255
Gene Alias
ALAS; ALAS-N; Alas-1; Alas-h
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Alas1em1flox/Cya mice (Catalog S-CKO-18255) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000141118
NCBI RefSeq
NM_020559.2
Target Region
Exon 6
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Alas1, also known as 5'-aminolevulinate synthase 1, is the rate-limiting enzyme in heme synthesis, a crucial process for maintaining intracellular heme levels [1,2,3,4,5]. Heme is involved in numerous biological processes, such as oxygen transport, electron transfer, and enzyme catalysis. Thus, Alas1 is of great biological importance in various tissues and organisms. Genetic models, like gene knockout mouse models, can be valuable tools to study its function.
In zebrafish, Alas1 -deficient models showed proper neutrophil initiation, but maturation was blocked due to heme deficiency, leading to lipid storage and granule formation deficiencies, as well as loss of heme-dependent granule protein activities, impairing bactericidal ability and augmenting inflammatory responses [3]. In mice, heterozygous disruption of Alas1 caused an accelerated age-dependent reduction in free heme, but not total heme, in skeletal muscle and liver, which could be related to a prediabetic phenotype and mitochondrial abnormality [5]. In cultured cardiomyocytes, doxorubicin decreased Alas1 abundance, disrupting heme synthesis, causing iron overload and ferroptosis, while Alas1 overexpression prevented this outcome [1]. In colorectal cancer cell lines, suppressing Alas1 inhibited cell proliferation and metastasis [4].
In conclusion, Alas1 is essential for heme synthesis, which in turn impacts various biological functions. Studies using gene knockout or knockdown models in zebrafish and mice have revealed its role in neutrophil maturation, age-related heme changes, and disease-related processes like ferroptosis in cardiomyocytes and proliferation/metastasis in colorectal cancer. These findings provide insights into the mechanisms of related diseases and potential therapeutic targets.
References:
1. Abe, Ko, Ikeda, Masataka, Ide, Tomomi, Imai, Hirotaka, Tsutsui, Hiroyuki. 2022. Doxorubicin causes ferroptosis and cardiotoxicity by intercalating into mitochondrial DNA and disrupting Alas1-dependent heme synthesis. In Science signaling, 15, eabn8017. doi:10.1126/scisignal.abn8017. https://pubmed.ncbi.nlm.nih.gov/36318618/
2. Li, Wentao, Li, Kunyu, He, Xiaoyun, Liu, Yufang, Chu, Mingxing. 2023. ALAS1 associated with goat kidding number trait was regulated by the transcription factor ASCL2 to affect granulosa cell proliferation. In Animal genetics, 54, 189-198. doi:10.1111/age.13287. https://pubmed.ncbi.nlm.nih.gov/36632647/
3. Lian, Junwei, Chen, Jiakui, Wang, Kun, Zhang, Wenqing, Zhang, Yiyue. 2018. Alas1 is essential for neutrophil maturation in zebrafish. In Haematologica, 103, 1785-1795. doi:10.3324/haematol.2018.194316. https://pubmed.ncbi.nlm.nih.gov/29954941/
4. Zhao, Yalei, Zhang, Xiaoyun, Liu, Yabin, Han, Mei, Li, Binghui. . Inhibition of ALAS1 activity exerts anti-tumour effects on colorectal cancer in vitro. In Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association, 26, 144-152. doi:10.4103/sjg.SJG_477_19. https://pubmed.ncbi.nlm.nih.gov/32270771/
5. van Wijk, Koen, Akabane, Takeru, Kimura, Tomohiro, Nakajima, Motowo, Nakajima, Osamu. 2020. Heterozygous disruption of ALAS1 in mice causes an accelerated age-dependent reduction in free heme, but not total heme, in skeletal muscle and liver. In Archives of biochemistry and biophysics, 697, 108721. doi:10.1016/j.abb.2020.108721. https://pubmed.ncbi.nlm.nih.gov/33307066/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen