C57BL/6JCya-Mfge8em1flox/Cya
Common Name
Mfge8-flox
Product ID
S-CKO-18375
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-17304-Mfge8-B6J-VB
Status
When using this mouse strain in a publication, please cite “Mfge8-flox Mouse (Catalog S-CKO-18375) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mfge8-flox
Strain ID
CKOCMP-17304-Mfge8-B6J-VB
Gene Name
Product ID
S-CKO-18375
Gene Alias
P47, MP47, Mfgm, SED1, MFG-E8, lactadherin
Background
C57BL/6JCya
Gene Full Name
milk fat globule EGF and factor V/VIII domain containing
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 7 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000032825
NCBI Transcript ID
NM_008594
Target Region
Exon 2~6
Size of Effective Region
~3.8 kb
Overview of Gene Research
Mfge8, also known as Milk fat globule-epidermal growth factor 8, is a secreted protein with diverse functions. It binds to integrin families, such as αVβ3/αVβ5, α8β1, and integrin β3, and is involved in multiple signaling pathways including PI3K/AKT, ERK/AKT, and integrin-mediated signaling. It is important in processes like angiogenesis, fibrosis, efferocytosis, insulin signaling, neurogenesis, and cancer progression [1-6].
In esophageal squamous cell carcinoma, MFGE8 secreted by cancer-associated fibroblasts promotes angiogenesis, tumor proliferation, drug resistance, and metastasis by activating PI3K/AKT and ERK/AKT pathways, and targeting it can inhibit ESCC growth and metastasis [1].
In intestinal fibrosis, MFGE8 shows antifibrotic effects by inhibiting ECM production in normal control human intestinal myofibroblasts, acting through integrin-mediated signaling pathways [2].
In colorectal cancer, CRC-derived extracellular vesicles containing MFGE8 facilitate macrophage efferocytosis of cisplatin-induced apoptotic CRC cells, contributing to cisplatin resistance [3].
MFGE8 also inhibits insulin signaling through PTP1B, with serum levels correlating with insulin resistance in humans [4].
In rats with experimental subarachnoid hemorrhage, MFGE8 promotes adult hippocampal neurogenesis via the integrin β3/Akt signaling pathway [5].
In gastric cancer, MFGE8 promotes cancer progression by activating the IL-6/JAK/STAT3 signaling [6].
In conclusion, Mfge8 is involved in a wide range of biological functions, with its dysregulation contributing to various disease conditions such as cancer, fibrosis, and metabolic disorders. The study of Mfge8 using in vivo models helps to understand its role in these diseases, potentially providing new therapeutic targets for treatment [1-6].
References：
1. Liu, Beilei, Zhang, Baifeng, Qi, Jiali, Wang, Xin, Guan, Xin-Yuan. 2023. Targeting MFGE8 secreted by cancer-associated fibroblasts blocks angiogenesis and metastasis in esophageal squamous cell carcinoma. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2307914120. doi:10.1073/pnas.2307914120. https://pubmed.ncbi.nlm.nih.gov/37816055/
2. Lin, Sinan, Wang, Jie, Mukherjee, Pranab K, Fiocchi, Claudio, Rieder, Florian. 2024. Milk fat globule-epidermal growth factor 8 (MFGE8) prevents intestinal fibrosis. In Gut, 73, 1110-1123. doi:10.1136/gutjnl-2022-328608. https://pubmed.ncbi.nlm.nih.gov/38378253/
3. Ma, Zhixin, Sun, Yu, Yu, Yang, Xiang, Xi, Li, Zhigang. 2024. Extracellular vesicles containing MFGE8 from colorectal cancer facilitate macrophage efferocytosis. In Cell communication and signaling : CCS, 22, 295. doi:10.1186/s12964-024-01669-9. https://pubmed.ncbi.nlm.nih.gov/38802814/
4. Datta, Ritwik, Podolsky, Michael J, Yang, Christopher D, Lizama, Carlos O, Atabai, Kamran. 2023. MFGE8 inhibits insulin signaling through PTP1B. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.30.542928. https://pubmed.ncbi.nlm.nih.gov/37398282/
5. Li, Zhen-Yan, Yang, Xian, Wang, Ji-Kai, Liu, Fei, Zuo, Yu-Chun. 2024. MFGE8 promotes adult hippocampal neurogenesis in rats following experimental subarachnoid hemorrhage via modifying the integrin β3/Akt signaling pathway. In Cell death discovery, 10, 359. doi:10.1038/s41420-024-02132-x. https://pubmed.ncbi.nlm.nih.gov/39128910/
6. Ding, Long-Long, Zhang, Meng, Zhang, Tao, Liu, Hui, Liu, Peng-Fei. 2024. MFGE8 promotes gastric cancer progression by activating the IL-6/JAK/STAT3 signaling. In Cellular signalling, 125, 111486. doi:10.1016/j.cellsig.2024.111486. https://pubmed.ncbi.nlm.nih.gov/39490801/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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