C57BL/6JCya-Pik3ap1em1flox/Cya
Common Name:
Pik3ap1-flox
Product ID:
S-CKO-18534
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pik3ap1-flox
Strain ID
CKOCMP-83490-Pik3ap1-B6J-VB
Gene Name
Product ID
S-CKO-18534
Gene Alias
1810044J04Rik; BCAP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pik3ap1em1flox/Cya mice (Catalog S-CKO-18534) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059672
NCBI RefSeq
NM_031376
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Pik3ap1, also known as B-cell adapter for PI3K (BCAP), plays a crucial role in the PI3K/AKT signaling pathway [1,2,3,4,5,6]. This gene is important for various biological processes such as the regulation of immune responses and cell proliferation [2,3,4,5]. Genetic models, like gene knockout mouse models, can be used to study its functions in vivo.
In macrophage-specific BCAP-deficient mice, they fail to recover from dextran sulfate sodium-induced colitis due to prolonged intestinal inflammation and impaired tissue repair, indicating that Pik3ap1 is essential for the transition of macrophages from an inflammatory to a reparative state [1]. In gastric epithelial cells (GES-1) stimulated by Helicobacter pylori, hypermethylation of the Pik3ap1 promoter leads to down-regulation of Pik3ap1, which in turn affects the expression of downstream genes like AKT and the immunoinflammatory gene IL-6, and also impacts cell proliferation [2]. In cervical cancer, increased expression of Pik3ap1 is observed, and silencing it inhibits cervical cancer progression [5].
In conclusion, Pik3ap1 is a key regulator in the PI3K/AKT signaling pathway and is involved in important biological processes such as macrophage state transition, immune response regulation, and cell proliferation. Its dysregulation is associated with diseases like colitis, gastric inflammation, and cervical cancer. The study of Pik3ap1 using gene knockout models has provided valuable insights into its functions in these disease-related biological processes.
References:
1. Irizarry-Caro, Ricardo A, McDaniel, Margaret M, Overcast, Garrett R, Troutman, Ty Dale, Pasare, Chandrashekhar. 2020. TLR signaling adapter BCAP regulates inflammatory to reparatory macrophage transition by promoting histone lactylation. In Proceedings of the National Academy of Sciences of the United States of America, 117, 30628-30638. doi:10.1073/pnas.2009778117. https://pubmed.ncbi.nlm.nih.gov/33199625/
2. Zhang, Andong, Yan, Shiying, Cao, Mei, Hu, Guoku, Zhao, Jian. 2020. Abnormal methylation of PIK3AP1 was involved in regulating the immune inflammatory response of GES-1 cells induced by Helicobacter pylori. In Biochemical and biophysical research communications, 524, 36-42. doi:10.1016/j.bbrc.2020.01.007. https://pubmed.ncbi.nlm.nih.gov/31980170/
3. Zhang, Feifei, Li, Kaitao, Yao, Xueqing, Xu, Lijun, Zhao, Liang. 2019. A miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance in gastric cancer. In EBioMedicine, 44, 311-321. doi:10.1016/j.ebiom.2019.05.003. https://pubmed.ncbi.nlm.nih.gov/31078520/
4. Li, Jingyan, Zhang, Zhanlei, Hu, Jieting, Zhang, Hong, Jiang, Ningyi. 2021. MiR-1246 regulates the PI3K/AKT signaling pathway by targeting PIK3AP1 and inhibits thyroid cancer cell proliferation and tumor growth. In Molecular and cellular biochemistry, 477, 649-661. doi:10.1007/s11010-021-04290-3. https://pubmed.ncbi.nlm.nih.gov/34870753/
5. Tong, Wenjuan, Zhang, Huiming. 2021. Overexpression of long non-coding RNA WT1-AS or silencing of PIK3AP1 are inhibitory to cervical cancer progression. In Cell cycle (Georgetown, Tex.), 20, 2583-2596. doi:10.1080/15384101.2021.1991106. https://pubmed.ncbi.nlm.nih.gov/34839795/
6. Liu, Gao, Sun, Bao-Ye, Sun, Jian, Yi, Yong, Qiu, Shuang-Jian. 2021. BRG1 regulates lipid metabolism in hepatocellular carcinoma through the PIK3AP1/PI3K/AKT pathway by mediating GLMP expression. In Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 54, 692-700. doi:10.1016/j.dld.2021.05.002. https://pubmed.ncbi.nlm.nih.gov/34158256/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen