C57BL/6JCya-Nr0b1em1flox/Cya
Common Name:
Nr0b1-flox
Product ID:
S-CKO-18653
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nr0b1-flox
Strain ID
CKOCMP-11614-Nr0b1-B6J-VB
Gene Name
Product ID
S-CKO-18653
Gene Alias
AHX; Ahc; Ahch; DAX-1; Dax1; mDax1-134; mDax1-218; mDax1-225; mDax1-249; mDax1-292; mDax1-297; mDax1-300; mDax1-308; mDax1-318; mDax1-329; mDax1-337; mDax1-344; mDax1-38; mDax1-404; mDax1-472
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nr0b1em1flox/Cya mice (Catalog S-CKO-18653) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026036
NCBI RefSeq
NM_007430
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Nr0b1, also known as DAX1, is a nuclear receptor gene encoding a transcriptional factor that plays a key role in adrenal and reproductive development in vertebrates [3,5,7,8]. It is involved in multiple biological processes, such as neural progenitor regulation, sex determination, and cell survival regulation, potentially through its influence on pathways like Notch signaling [1,9].
In zebrafish, nr0b1 disruption causes abnormal hypothalamic functions, including abnormal gonadotropin expression, changes in fertilization rate and post-fasting food intake. Loss of nr0b1 leads to an altered number of specific neurons in the hypothalamic arcuate region, along with reduced progenitor cell proliferation and decreased Notch pathway gene expression [1]. In the same species, nr0b1 mutation results in female-to-male sex reversal due to abnormal gonadal proliferation and differentiation [9]. In lung cancer cells, NR0B1 depletion restrains xenograft tumor growth and facilitates ferroptosis, while in hepatocellular carcinoma, NR0B1 promotes autophagy, inhibits apoptosis, and augments sorafenib resistance [2,6]. Also, in humans, NR0B1 mutations are associated with congenital adrenal hyperplasia, X-linked adrenal hypoplasia congenita, and hypogonadotropic hypogonadism [3,4,5,7,8].
In summary, Nr0b1 is crucial for normal hypothalamic neuron development, sex development, and is involved in various disease-related processes such as cancer cell survival and ferroptosis regulation. Studies in genetic models like zebrafish have significantly contributed to understanding Nr0b1's functions and its role in diseases like adrenal and reproductive disorders [1,3,4,5,6,7,8,9].
References:
1. Zhang, Wei, Li, Yan, Chen, Sijie, Chen, Lili, Peng, Gang. 2021. nr0b1 (DAX1) loss of function in zebrafish causes hypothalamic defects via abnormal progenitor proliferation and differentiation. In Journal of genetics and genomics = Yi chuan xue bao, 49, 217-229. doi:10.1016/j.jgg.2021.08.019. https://pubmed.ncbi.nlm.nih.gov/34606992/
2. Zhang, Xin-Yue, Zhang, Hao, Hu, Si-Jing, Yang, Yuan, Liu, Yun-Qiang. 2023. NR0B1 suppresses ferroptosis through upregulation of NRF2/c-JUN-CBS signaling pathway in lung cancer cells. In American journal of cancer research, 13, 5174-5196. doi:. https://pubmed.ncbi.nlm.nih.gov/38058844/
3. Suntharalingham, Jenifer P, Buonocore, Federica, Duncan, Andrew J, Achermann, John C. 2015. DAX-1 (NR0B1) and steroidogenic factor-1 (SF-1, NR5A1) in human disease. In Best practice & research. Clinical endocrinology & metabolism, 29, 607-19. doi:10.1016/j.beem.2015.07.004. https://pubmed.ncbi.nlm.nih.gov/26303087/
4. Ravel, Célia, Hyon, Capucine, Siffroi, Jean-Pierre, Christin-Maitre, Sophie. 2014. Are human male patients with DAX1/NR0B1 mutations infertile? In Annales d'endocrinologie, 75, 126-7. doi:10.1016/j.ando.2014.03.003. https://pubmed.ncbi.nlm.nih.gov/24751136/
5. Phelan, J K, McCabe, E R. . Mutations in NR0B1 (DAX1) and NR5A1 (SF1) responsible for adrenal hypoplasia congenita. In Human mutation, 18, 472-87. doi:. https://pubmed.ncbi.nlm.nih.gov/11748841/
6. Tan, Xiao Lan, Wang, Zhaokun, Liao, Shunyao, Yang, Yuan, Liu, Yun Qiang. 2023. NR0B1 augments sorafenib resistance in hepatocellular carcinoma through promoting autophagy and inhibiting apoptosis. In Cancer science, 115, 465-476. doi:10.1111/cas.16029. https://pubmed.ncbi.nlm.nih.gov/37991109/
7. Fan, Da-Bei, Li, Li, Zhang, Hao-Hao. 2021. Nonsense variant of NR0B1 causes hormone disorders associated with congenital adrenal hyperplasia. In Scientific reports, 11, 16066. doi:10.1038/s41598-021-95642-y. https://pubmed.ncbi.nlm.nih.gov/34373561/
8. El-Khairi, Ranna, Martinez-Aguayo, Alejandro, Ferraz-de-Souza, Bruno, Lin, Lin, Achermann, John C. 2010. Role of DAX-1 (NR0B1) and steroidogenic factor-1 (NR5A1) in human adrenal function. In Endocrine development, 20, 38-46. doi:10.1159/000321213. https://pubmed.ncbi.nlm.nih.gov/21164257/
9. Chen, Sijie, Zhang, Hefei, Wang, Fenghua, Zhang, Wei, Peng, Gang. 2016. nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation. In Molecular and cellular endocrinology, 433, 105-16. doi:10.1016/j.mce.2016.06.005. https://pubmed.ncbi.nlm.nih.gov/27267667/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen