Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Nr0b1em1flox/Cya
Common Name:
Nr0b1-flox
Product ID:
S-CKO-18653
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nr0b1-flox
Strain ID
CKOCMP-11614-Nr0b1-B6J-VB
Gene Name
Nr0b1
Product ID
S-CKO-18653
Gene Alias
AHX; Ahc; Ahch; DAX-1; Dax1; mDax1-134; mDax1-218; mDax1-225; mDax1-249; mDax1-292; mDax1-297; mDax1-300; mDax1-308; mDax1-318; mDax1-329; mDax1-337; mDax1-344; mDax1-38; mDax1-404; mDax1-472
Background
C57BL/6JCya
NCBI ID
11614
Modification
Conditional knockout
Chromosome
X
Phenotype
MGI:1352460
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nr0b1em1flox/Cya mice (Catalog S-CKO-18653) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026036
NCBI RefSeq
NM_007430
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Nr0b1, also known as DAX1, is a nuclear receptor gene encoding a transcriptional factor that plays a key role in adrenal and reproductive development in vertebrates [3,5,7,8]. It is involved in multiple biological processes, such as neural progenitor regulation, sex determination, and cell survival regulation, potentially through its influence on pathways like Notch signaling [1,9].

In zebrafish, nr0b1 disruption causes abnormal hypothalamic functions, including abnormal gonadotropin expression, changes in fertilization rate and post-fasting food intake. Loss of nr0b1 leads to an altered number of specific neurons in the hypothalamic arcuate region, along with reduced progenitor cell proliferation and decreased Notch pathway gene expression [1]. In the same species, nr0b1 mutation results in female-to-male sex reversal due to abnormal gonadal proliferation and differentiation [9]. In lung cancer cells, NR0B1 depletion restrains xenograft tumor growth and facilitates ferroptosis, while in hepatocellular carcinoma, NR0B1 promotes autophagy, inhibits apoptosis, and augments sorafenib resistance [2,6]. Also, in humans, NR0B1 mutations are associated with congenital adrenal hyperplasia, X-linked adrenal hypoplasia congenita, and hypogonadotropic hypogonadism [3,4,5,7,8].

In summary, Nr0b1 is crucial for normal hypothalamic neuron development, sex development, and is involved in various disease-related processes such as cancer cell survival and ferroptosis regulation. Studies in genetic models like zebrafish have significantly contributed to understanding Nr0b1's functions and its role in diseases like adrenal and reproductive disorders [1,3,4,5,6,7,8,9].

References:
1. Zhang, Wei, Li, Yan, Chen, Sijie, Chen, Lili, Peng, Gang. 2021. nr0b1 (DAX1) loss of function in zebrafish causes hypothalamic defects via abnormal progenitor proliferation and differentiation. In Journal of genetics and genomics = Yi chuan xue bao, 49, 217-229. doi:10.1016/j.jgg.2021.08.019. https://pubmed.ncbi.nlm.nih.gov/34606992/
2. Zhang, Xin-Yue, Zhang, Hao, Hu, Si-Jing, Yang, Yuan, Liu, Yun-Qiang. 2023. NR0B1 suppresses ferroptosis through upregulation of NRF2/c-JUN-CBS signaling pathway in lung cancer cells. In American journal of cancer research, 13, 5174-5196. doi:. https://pubmed.ncbi.nlm.nih.gov/38058844/
3. Suntharalingham, Jenifer P, Buonocore, Federica, Duncan, Andrew J, Achermann, John C. 2015. DAX-1 (NR0B1) and steroidogenic factor-1 (SF-1, NR5A1) in human disease. In Best practice & research. Clinical endocrinology & metabolism, 29, 607-19. doi:10.1016/j.beem.2015.07.004. https://pubmed.ncbi.nlm.nih.gov/26303087/
4. Ravel, Célia, Hyon, Capucine, Siffroi, Jean-Pierre, Christin-Maitre, Sophie. 2014. Are human male patients with DAX1/NR0B1 mutations infertile? In Annales d'endocrinologie, 75, 126-7. doi:10.1016/j.ando.2014.03.003. https://pubmed.ncbi.nlm.nih.gov/24751136/
5. Phelan, J K, McCabe, E R. . Mutations in NR0B1 (DAX1) and NR5A1 (SF1) responsible for adrenal hypoplasia congenita. In Human mutation, 18, 472-87. doi:. https://pubmed.ncbi.nlm.nih.gov/11748841/
6. Tan, Xiao Lan, Wang, Zhaokun, Liao, Shunyao, Yang, Yuan, Liu, Yun Qiang. 2023. NR0B1 augments sorafenib resistance in hepatocellular carcinoma through promoting autophagy and inhibiting apoptosis. In Cancer science, 115, 465-476. doi:10.1111/cas.16029. https://pubmed.ncbi.nlm.nih.gov/37991109/
7. Fan, Da-Bei, Li, Li, Zhang, Hao-Hao. 2021. Nonsense variant of NR0B1 causes hormone disorders associated with congenital adrenal hyperplasia. In Scientific reports, 11, 16066. doi:10.1038/s41598-021-95642-y. https://pubmed.ncbi.nlm.nih.gov/34373561/
8. El-Khairi, Ranna, Martinez-Aguayo, Alejandro, Ferraz-de-Souza, Bruno, Lin, Lin, Achermann, John C. 2010. Role of DAX-1 (NR0B1) and steroidogenic factor-1 (NR5A1) in human adrenal function. In Endocrine development, 20, 38-46. doi:10.1159/000321213. https://pubmed.ncbi.nlm.nih.gov/21164257/
9. Chen, Sijie, Zhang, Hefei, Wang, Fenghua, Zhang, Wei, Peng, Gang. 2016. nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation. In Molecular and cellular endocrinology, 433, 105-16. doi:10.1016/j.mce.2016.06.005. https://pubmed.ncbi.nlm.nih.gov/27267667/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest