C57BL/6JCya-Ddx54em1flox/Cya
Common Name
Ddx54-flox
Product ID
S-CKO-19315
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-71990-Ddx54-B6J-VB
When using this mouse strain in a publication, please cite “Ddx54-flox Mouse (Catalog S-CKO-19315) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ddx54-flox
Strain ID
CKOCMP-71990-Ddx54-B6J-VB
Gene Name
Product ID
S-CKO-19315
Gene Alias
2410015A15Rik, DP97
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031598
NCBI RefSeq
NM_028041
Target Region
Exon 3~10
Size of Effective Region
~3.3 kb
Overview of Gene Research
Ddx54, also known as Dbp10, is a DEAD-box RNA helicase. It plays crucial roles in multiple biological processes. It is involved in ribosome biogenesis, where it initiates peptidyl transferase center formation during 60S ribosome biogenesis [4,5]. It also functions in post-transcriptional gene regulation, such as regulating transcriptome dynamics during the DNA damage response [3]. Additionally, it participates in signaling pathways like the p65 and AKT signaling pathway [1].
In a Ddx54 knockout mouse model (Ddx54fl/fl;MBP-Cre), after normal postnatal myelination, mice gradually develop behavioral and learning abnormalities, inner myelin sheath breakdown, loss of myelinated axons, oligodendrocyte apoptosis, astrocyte and microglia activation, and die within 7 months. This indicates that Ddx54 is indispensable for myelin homeostasis [2].
In conclusion, Ddx54 is essential for ribosome biogenesis, myelin homeostasis, and post-transcriptional gene regulation during DNA damage response. The Ddx54 knockout mouse model has provided insights into its role in myelin-related diseases, highlighting its importance in understanding the mechanisms underlying these conditions [2].
References：
1. Yu, Yi, Wang, Jing-Long, Meng, Li-Li, Fu, Guo-Hui, Zu, Li-Dong. 2021. DDX54 Plays a Cancerous Role Through Activating P65 and AKT Signaling Pathway in Colorectal Cancer. In Frontiers in oncology, 11, 650360. doi:10.3389/fonc.2021.650360. https://pubmed.ncbi.nlm.nih.gov/33968751/
2. Oizumi, Hiroaki, Miyamoto, Yuki, Seiwa, Chika, Yamauchi, Junji, Asou, Hiroaki. 2023. Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockout. In iScience, 26, 107448. doi:10.1016/j.isci.2023.107448. https://pubmed.ncbi.nlm.nih.gov/37720086/
3. Milek, Miha, Imami, Koshi, Mukherjee, Neelanjan, Selbach, Matthias, Landthaler, Markus. 2017. DDX54 regulates transcriptome dynamics during DNA damage response. In Genome research, 27, 1344-1359. doi:10.1101/gr.218438.116. https://pubmed.ncbi.nlm.nih.gov/28596291/
4. Cruz, Victor E, Weirich, Christine S, Peddada, Nagesh, Erzberger, Jan P. 2024. The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis. In Nature communications, 15, 3296. doi:10.1038/s41467-024-47616-7. https://pubmed.ncbi.nlm.nih.gov/38632236/
5. Cruz, Victor E, Weirich, Christine S, Peddada, Nagesh, Erzberger, Jan P. 2023. The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.11.01.565222. https://pubmed.ncbi.nlm.nih.gov/37961218/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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