C57BL/6JCya-Clic3em1flox/Cya
Common Name
Clic3-flox
Product ID
S-CKO-19443
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-69454-Clic3-B6J-VA
When using this mouse strain in a publication, please cite “Clic3-flox Mouse (Catalog S-CKO-19443) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clic3-flox
Strain ID
CKOCMP-69454-Clic3-B6J-VA
Gene Name
Product ID
S-CKO-19443
Gene Alias
2300003G24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000114265
NCBI RefSeq
NM_027085
Target Region
Exon 2~6
Size of Effective Region
~1.6 kb
Overview of Gene Research
CLIC3, chloride intracellular channel 3, belongs to the glutathione-S-transferase (GSTs) superfamily. It regulates chloride ion concentration, cell membrane potential, trans-epithelial transport, cell volume, and apoptotic processes [6]. It is involved in diverse biological pathways such as the cell cycle, focal adhesion, extracellular matrix (ECM) receptor interaction, and the P53 signaling pathway [3].
In cancer research, CLIC3 has been extensively studied. In bladder cancer, it promotes cell proliferation by interacting with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA, leading to reduced p21 expression [1]. High CLIC3 mRNA expression in bladder cancer is associated with poor prognosis and is related to adverse clinicopathological factors [3]. In breast cancer, it controls the recycling of late endosomal MT1-MMP, dictating invasion and metastasis in estrogen receptor-negative breast cancer cells [5]. In pancreatic ductal adenocarcinoma, CLIC3, along with Rab25, promotes integrin recycling from late endosomes/lysosomes, and its expression predicts lymph node metastasis and poor prognosis [9]. In gastric cancer, it functions as a Cl-channel in the plasma membrane, and decreased expression is associated with unfavorable prognosis [4]. In salivary gland mucoepidermoid carcinoma, hypomethylation of the CLIC3 promoter leads to its overexpression [10]. Secreted CLIC3 from cancer-associated fibroblasts promotes angiogenesis and cancer cell invasiveness through its glutathione-dependent oxidoreductase activity [2].
In summary, CLIC3 plays crucial roles in cancer progression, being involved in processes like cell proliferation, invasion, and metastasis across multiple cancer types. Additionally, it may have a role in abnormal placental function in pregnancy disorders such as fetal growth restriction and pre-eclampsia [6], and is identified as an immune-related hub gene in schizophrenia, potentially serving as a diagnostic biomarker and therapeutic target [7]. Recently, it has been shown to mediate fibroblast cellular senescence by interacting with ERK7 [8].
References：
1. Shuai, Yujun, Zhang, Hui, Liu, Changhao, Chen, Hebing, Jiang, Guosong. 2024. CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression. In Cell death & disease, 15, 9. doi:10.1038/s41419-023-06373-z. https://pubmed.ncbi.nlm.nih.gov/38182571/
2. Hernandez-Fernaud, Juan R, Ruengeler, Elena, Casazza, Andrea, Norman, Jim C, Zanivan, Sara. 2017. Secreted CLIC3 drives cancer progression through its glutathione-dependent oxidoreductase activity. In Nature communications, 8, 14206. doi:10.1038/ncomms14206. https://pubmed.ncbi.nlm.nih.gov/28198360/
3. Chen, Mei, Zhang, Shufang, Wen, Xiaohong, Cao, Hui, Gao, Yuanhui. 2020. Prognostic value of CLIC3 mRNA overexpression in bladder cancer. In PeerJ, 8, e8348. doi:10.7717/peerj.8348. https://pubmed.ncbi.nlm.nih.gov/31934512/
4. Kawai, Shunsuke, Fujii, Takuto, Shimizu, Takahiro, Sakai, Hideki, Fujii, Tsutomu. 2020. Pathophysiological properties of CLIC3 chloride channel in human gastric cancer cells. In The journal of physiological sciences : JPS, 70, 15. doi:10.1186/s12576-020-00740-7. https://pubmed.ncbi.nlm.nih.gov/32066374/
5. Macpherson, Iain R, Rainero, Elena, Mitchell, Louise E, Timpson, Paul, Norman, Jim C. 2014. CLIC3 controls recycling of late endosomal MT1-MMP and dictates invasion and metastasis in breast cancer. In Journal of cell science, 127, 3893-901. doi:10.1242/jcs.135947. https://pubmed.ncbi.nlm.nih.gov/25015290/
6. Murthi, P, Stevenson, J L, Money, T T, Brennecke, S P, Gude, N M. 2012. Placental CLIC3 is increased in fetal growth restriction and pre-eclampsia affected human pregnancies. In Placenta, 33, 741-4. doi:10.1016/j.placenta.2012.06.011. https://pubmed.ncbi.nlm.nih.gov/22795578/
7. Zhu, Xiaoli, Wang, Chuan-Lan, Yu, Jian-Feng, Tang, Xiaowei, Pan, Bo. 2023. Identification of immune-related biomarkers in peripheral blood of schizophrenia using bioinformatic methods and machine learning algorithms. In Frontiers in cellular neuroscience, 17, 1256184. doi:10.3389/fncel.2023.1256184. https://pubmed.ncbi.nlm.nih.gov/37841288/
8. Luan, Changjiao, Gao, Yue, Zhao, Jun, Gong, Weijuan, Ma, Xingjie. 2025. Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. In Communications biology, 8, 51. doi:10.1038/s42003-025-07482-5. https://pubmed.ncbi.nlm.nih.gov/39809890/
9. Dozynkiewicz, Marta A, Jamieson, Nigel B, Macpherson, Iain, Caswell, Patrick T, Norman, Jim C. 2011. Rab25 and CLIC3 collaborate to promote integrin recycling from late endosomes/lysosomes and drive cancer progression. In Developmental cell, 22, 131-45. doi:10.1016/j.devcel.2011.11.008. https://pubmed.ncbi.nlm.nih.gov/22197222/
10. Wang, Zhiming, Ling, Shizhang, Rettig, Eleni, Fakhry, Carole, Ha, Patrick K. 2015. Epigenetic screening of salivary gland mucoepidermoid carcinoma identifies hypomethylation of CLIC3 as a common alteration. In Oral oncology, 51, 1120-5. doi:10.1016/j.oraloncology.2015.09.010. https://pubmed.ncbi.nlm.nih.gov/26490796/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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