C57BL/6JCya-Adarb1em1/Cya
Common Name:
Adarb1-KO
Product ID:
S-KO-00765
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Adarb1-KO
Strain ID
KOCMP-110532-Adarb1-B6J-VA
Gene Name
Product ID
S-KO-00765
Gene Alias
1700057H01Rik; Adar2; D10Bwg0447e; Red1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adarb1em1/Cya mice (Catalog S-KO-00765) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098374
NCBI RefSeq
NM_001024837.2
Target Region
Exon 4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Adarb1, also named ADAR2, is an adenosine-to-inosine (A-to-I) RNA-editing enzyme. A-to-I RNA editing is a crucial post-transcriptional modification, which can affect RNA stability, translation, and protein-coding potential, thus participating in various biological processes. ADARB1 has been shown to be involved in regulating neurotransmitter receptors and may be associated with the mitochondrial respiratory chain complex [2].
In cancer research, downregulated ADARB1 expression was found in lung adenocarcinoma, lung squamous cell carcinoma, and ovarian cancer, and it was related to tumor cell metastasis, shorter survival times, and cell proliferation [1,3,6]. In glioblastoma, ADARB1 was upregulated and involved in AKT-mediated temozolomide resistance [2]. In hepatocellular carcinoma treated with transarterial chemoembolization, ADARB1 polymorphisms were associated with prognosis [7].
In neurological research, bi-allelic variants in ADARB1 were associated with microcephaly, intellectual disability, and epilepsy, as these variants led to reduced ADAR2 editing activity [4,8].
In circadian rhythm research, Adarb1-knockout mice exhibited attenuated mRNA rhythms, short-period rhythms in locomotor activity, and abnormal accumulation of CRY2, indicating ADARB1's role in post-transcriptional regulation of the circadian clock [5].
In conclusion, ADARB1 is an essential RNA-editing enzyme with a wide-reaching impact on biological processes. Its role in various diseases, especially cancer and neurological disorders, has been revealed through studies including gene-knockout models. Understanding ADARB1's function helps to better understand disease mechanisms and may provide potential therapeutic targets.
References:
1. Wang, Xiang, Xu, Zhijie, Ren, Xinxin, Gong, Zhicheng, Yan, Yuanliang. 2019. Function of low ADARB1 expression in lung adenocarcinoma. In PloS one, 14, e0222298. doi:10.1371/journal.pone.0222298. https://pubmed.ncbi.nlm.nih.gov/31491024/
2. Lu, Can, Chen, Xi, Yan, Yuanliang, Xu, Zhijie, Peng, Jinwu. 2022. Aberrant Expression of ADARB1 Facilitates Temozolomide Chemoresistance and Immune Infiltration in Glioblastoma. In Frontiers in pharmacology, 13, 768743. doi:10.3389/fphar.2022.768743. https://pubmed.ncbi.nlm.nih.gov/35177985/
3. Wang, Xiang, Ren, Xinxin, Liu, Wanli, Yan, Yuanliang, Xu, Zhijie. 2020. Role of downregulated ADARB1 in lung squamous cell carcinoma. In Molecular medicine reports, 21, 1517-1526. doi:10.3892/mmr.2020.10958. https://pubmed.ncbi.nlm.nih.gov/32016472/
4. Tan, Tiong Yang, Sedmík, Jiří, Fitzgerald, Mark P, Christodoulou, John, O'Connell, Mary A. 2020. Bi-allelic ADARB1 Variants Associated with Microcephaly, Intellectual Disability, and Seizures. In American journal of human genetics, 106, 467-483. doi:10.1016/j.ajhg.2020.02.015. https://pubmed.ncbi.nlm.nih.gov/32220291/
5. Terajima, Hideki, Yoshitane, Hikari, Ozaki, Haruka, Iwasaki, Wataru, Fukada, Yoshitaka. 2016. ADARB1 catalyzes circadian A-to-I editing and regulates RNA rhythm. In Nature genetics, 49, 146-151. doi:10.1038/ng.3731. https://pubmed.ncbi.nlm.nih.gov/27893733/
6. Zhu, Wei, Xu, Zhijie, Huang, Meiyuan, Chen, Xi, Yan, Yuanliang. 2021. Downregulated ADARB1 Facilitates Cell Proliferation, Invasion and has Effect on the Immune Regulation in Ovarian Cancer. In Frontiers in bioengineering and biotechnology, 9, 792911. doi:10.3389/fbioe.2021.792911. https://pubmed.ncbi.nlm.nih.gov/35004651/
7. Zeng, Jiajia, Han, Linyu, Wang, Teng, Li, Ziqiang, Yang, Ming. 2023. The Allelic Expression of RNA Editing Gene ADARB1 in Hepatocellular Carcinoma Treated with Transarterial Chemoembolization. In Pharmacogenomics and personalized medicine, 16, 229-238. doi:10.2147/PGPM.S402115. https://pubmed.ncbi.nlm.nih.gov/36970122/
8. Maroofian, Reza, Sedmík, Jiří, Mazaheri, Neda, O'Connell, Mary A, Houlden, Henry. 2020. Biallelic variants in ADARB1, encoding a dsRNA-specific adenosine deaminase, cause a severe developmental and epileptic encephalopathy. In Journal of medical genetics, 58, 495-504. doi:10.1136/jmedgenet-2020-107048. https://pubmed.ncbi.nlm.nih.gov/32719099/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen