Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Csf1em1/Cya
Common Name:
Csf1-KO
Product ID:
S-KO-01658
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Csf1-KO
Strain ID
KOCMP-12977-Csf1-B6J-VA
Gene Name
Csf1
Product ID
S-KO-01658
Gene Alias
BAP025; Csfm; MCSF; Mhdabap25; PG-M-CSF; op
Background
C57BL/6JCya
NCBI ID
12977
Modification
Conventional knockout
Chromosome
3
Phenotype
MGI:1339753
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Csf1em1/Cya mice (Catalog S-KO-01658) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000014743
NCBI RefSeq
NM_007778
Target Region
Exon 6
Size of Effective Region
~1.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Csf1, also known as macrophage colony-stimulating factor 1, is the primary growth factor essential for the control of monocyte and macrophage differentiation, survival, proliferation and renewal [4]. It is a key regulator in pathways related to immune cell function, tissue repair, and bone remodeling. Csf1 binds to its receptor Csf1R, activating downstream signaling cascades that are crucial for the normal development and function of macrophages and related cell populations [1,2,4,5,6,7].

In a Csf1 conditional knockout (CKO) mouse model using AdipoqCre to deplete Csf1 in marrow adipogenic lineage precursors (MALPs), the mice had increased femoral trabecular bone mass due to diminished osteoclasts in the femoral secondary spongiosa region. These mice were also resistant to LPS-induced calvarial osteolysis, and had reduced bone marrow cellularity, hematopoietic progenitors, and macrophages. This indicates that MALPs-derived Csf1 is required for osteoclast formation and hematopoiesis in bone [3].

In conclusion, Csf1 plays essential roles in the development and maintenance of monocyte-macrophage lineages, bone remodeling, and hematopoiesis. The Csf1 CKO mouse model has provided insights into its role in bone-related diseases, highlighting its potential as a therapeutic target for conditions involving abnormal bone metabolism and immune-related disorders [3].

References:

1. Fujiwara, Tomohiro, Yakoub, Mohamed A, Chandler, Andrew, Purdue, Ed, Healey, John H. 2021. CSF1/CSF1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in the Sarcoma Microenvironment. In Molecular cancer therapeutics, 20, 1388-1399. doi:10.1158/1535-7163.MCT-20-0591. https://pubmed.ncbi.nlm.nih.gov/34088832/

2. Huang, Yao-Hui, Cai, Kun, Xu, Peng-Peng, Ji, Meng-Meng, Zhao, Wei-Li. 2021. CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis. In Signal transduction and targeted therapy, 6, 10. doi:10.1038/s41392-020-00437-8. https://pubmed.ncbi.nlm.nih.gov/33431788/

3. Zhong, Leilei, Lu, Jiawei, Fang, Jiankang, Dyment, Nathanial, Qin, Ling. 2023. Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone. In eLife, 12, . doi:10.7554/eLife.82112. https://pubmed.ncbi.nlm.nih.gov/36779854/

4. Sehgal, Anuj, Irvine, Katharine M, Hume, David A. 2021. Functions of macrophage colony-stimulating factor (CSF1) in development, homeostasis, and tissue repair. In Seminars in immunology, 54, 101509. doi:10.1016/j.smim.2021.101509. https://pubmed.ncbi.nlm.nih.gov/34742624/

5. Wang, Qian, Wang, Jianhong, Xu, Ke, Luo, Zhibin. 2024. Targeting the CSF1/CSF1R signaling pathway: an innovative strategy for ultrasound combined with macrophage exhaustion in pancreatic cancer therapy. In Frontiers in immunology, 15, 1481247. doi:10.3389/fimmu.2024.1481247. https://pubmed.ncbi.nlm.nih.gov/39416792/

6. Zhou, Yamei, Zeng, Jia, Tu, Yuanyuan, Zhu, Manhui, Liu, Xiaojuan. . CSF1/CSF1R-mediated Crosstalk Between Choroidal Vascular Endothelial Cells and Macrophages Promotes Choroidal Neovascularization. In Investigative ophthalmology & visual science, 62, 37. doi:10.1167/iovs.62.3.37. https://pubmed.ncbi.nlm.nih.gov/33764399/

7. Assi, Tarek, Moussa, Tania, Ngo, Carine, Vibert, Julien, Cesne, Axel Le. 2025. Therapeutic advances in Tenosynovial giant cell Tumor: Targeting the CSF1/CSF1R axis. In Cancer treatment reviews, 134, 102904. doi:10.1016/j.ctrv.2025.102904. https://pubmed.ncbi.nlm.nih.gov/40020639/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest