C57BL/6JCya-Dclk1em1/Cya
Common Name
Dclk1-KO
Product ID
S-KO-01738
Backgroud
C57BL/6JCya
Strain ID
KOCMP-13175-Dclk1-B6J-VB
Status
When using this mouse strain in a publication, please cite “Dclk1-KO Mouse (Catalog S-KO-01738) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Dclk1-KO
Strain ID
KOCMP-13175-Dclk1-B6J-VB
Gene Name
Product ID
S-KO-01738
Gene Alias
Dcl, Dclk, Cpg16, Click-I, Dcamkl1, mKIAA0369, 1700113D08Rik, 2810480F11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000054237
NCBI RefSeq
NM_019978
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Overview of Gene Research
Dclk1, or Doublecortin-like kinase 1, is a microtubule-associated protein kinase. It is involved in neurogenesis and has been implicated in multiple biological processes. In cancer, it serves as a stem cell marker, regulating tumorigenesis, epithelial-mesenchymal transformation, DNA damage response, and tumor immune evasion through pathways like Notch, Wnt/β-catenin, and RAS [3,4].
In atherosclerosis, macrophage-specific Dclk1 deletion in ApoE-/-mice fed a high-fat diet (HFD) attenuated the disease by reducing inflammation. Mechanistically, Dclk1 in macrophages binds to IKKβ, phosphorylates it at S177/181, activates the NF-κB signaling pathway, and induces inflammatory gene expression [1]. In obesity-induced cardiomyopathy, macrophage-specific Dclk1 knockout, rather than cardiomyocyte-specific knockout, protected against HFD-induced heart dysfunction, hypertrophy, and fibrosis. Dclk1 deficiency suppressed RIP2/TAK1 activation and inflammatory responses in macrophages [2].
In conclusion, Dclk1 plays crucial roles in inflammation-related diseases such as atherosclerosis and obesity-induced cardiomyopathy. Gene knockout mouse models, especially macrophage-specific conditional knockout models, have revealed its pro-inflammatory mechanisms in these diseases, highlighting Dclk1 as a potential therapeutic target for these conditions.
References:
1. Huang, Zhuqi, Shen, Sirui, Han, Xue, Wu, Gaojun, Liang, Guang. 2023. Macrophage DCLK1 promotes atherosclerosis via binding to IKKβ and inducing inflammatory responses. In EMBO molecular medicine, 15, e17198. doi:10.15252/emmm.202217198. https://pubmed.ncbi.nlm.nih.gov/36896602/
2. Yang, Bin, Zhao, Yunjie, Luo, Wu, Wang, Yi, Liang, Guang. 2023. Macrophage DCLK1 promotes obesity-induced cardiomyopathy via activating RIP2/TAK1 signaling pathway. In Cell death & disease, 14, 419. doi:10.1038/s41419-023-05960-4. https://pubmed.ncbi.nlm.nih.gov/37443105/
3. Lu, Qin, Feng, Hailan, Chen, Hong, Yan, Zixing, Cao, Zhiyun. 2022. Role of DCLK1 in oncogenic signaling (Review). In International journal of oncology, 61, . doi:10.3892/ijo.2022.5427. https://pubmed.ncbi.nlm.nih.gov/36148883/
4. Ye, Liu, Liu, Beibei, Huang, Jingling, Xu, Yungen, Wang, Shuping. 2023. DCLK1 and its oncogenic functions: A promising therapeutic target for cancers. In Life sciences, 336, 122294. doi:10.1016/j.lfs.2023.122294. https://pubmed.ncbi.nlm.nih.gov/38007147/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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