C57BL/6JCya-Hbb-yem1/Cya
Common Name:
Hbb-y-KO
Product ID:
S-KO-02415
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hbb-y-KO
Strain ID
KOCMP-15135-Hbb-y-B6J-VA
Gene Name
Product ID
S-KO-02415
Gene Alias
Ey; Hby
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hbb-yem1/Cya mice (Catalog S-KO-02415) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033229
NCBI RefSeq
NM_008221
Target Region
Exon 1~3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Hbb-y, a gene encoding a beta-like embryonic chain of hemoglobin, is crucial in embryonic globin expression. Hemoglobin, with its globin chains, is essential for oxygen transport in the body, and thus Hbb-y plays a vital role in the normal physiological function during embryonic development. Mouse models are valuable genetic tools for studying Hbb-y [1,2,3,4].
In Gfi1b-deficient mice, which serve as a model for studying erythroid cell maturation and globin gene regulation, the Hbb-y gene was upregulated in TER119+ fetal liver cells during gestation (day 12.5-17.5 p.c.), and this increased expression was maintained in adult Gfi1b-deficient mice. This indicates that Gfi1b is a regulator of Hbb-y expression and suggests Hbb-y's role in erythroid maturation [4]. Additionally, exposure to certain androgen receptor antagonists like flutamide, linuron, and p,p'-DDE in rat fetal testes led to a reduction in Hbb-y expression, though the exact implications of this on testicular development are yet to be fully understood [5].
In conclusion, Hbb-y is important for embryonic globin expression and may be involved in erythroid cell maturation. The use of gene-modified mouse models, such as Gfi1b-deficient mice, has provided insights into Hbb-y's role in these biological processes. While its role in the context of androgen receptor antagonist-induced changes in the fetal testis is less clear, further research may clarify its significance in this and other disease-related scenarios.
References:
1. Brown, B A, Padgett, R W, Hardies, S C, Hutchison, C A, Edgell, M H. . beta-globin transcript found in induced murine erythroleukemia cells is homologous to the beta h0 and beta h1 genes. In Proceedings of the National Academy of Sciences of the United States of America, 79, 2753-7. doi:. https://pubmed.ncbi.nlm.nih.gov/6953428/
2. Padgett, R W, Loeb, D D, Snyder, L R, Edgell, M H, Hutchison, C A. . The molecular organization of the beta-globin complex of the deer mouse, Peromyscus maniculatus. In Molecular biology and evolution, 4, 30-45. doi:. https://pubmed.ncbi.nlm.nih.gov/3447002/
3. May, Andreas, Reifenberg, Kurt, Zechner, Ulrich, Haaf, Thomas. 2008. Asynchronous replication dynamics of imprinted and non-imprinted chromosome regions in early mouse embryos. In Experimental cell research, 314, 2788-95. doi:10.1016/j.yexcr.2008.07.009. https://pubmed.ncbi.nlm.nih.gov/18675801/
4. Vassen, Lothar, Beauchemin, Hugues, Lemsaddek, Wafaa, Trudel, Marie, Möröy, Tarik. 2014. Growth factor independence 1b (gfi1b) is important for the maturation of erythroid cells and the regulation of embryonic globin expression. In PloS one, 9, e96636. doi:10.1371/journal.pone.0096636. https://pubmed.ncbi.nlm.nih.gov/24800817/
5. Mu, Xueyan, Liu, Kejun, Kleymenova, Elena, Young, S Stanley, Gaido, Kevin W. . Gene expression profiling of androgen receptor antagonists in the rat fetal testis reveals few common gene targets. In Journal of biochemical and molecular toxicology, 20, 7-17. doi:. https://pubmed.ncbi.nlm.nih.gov/16498641/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen