C57BL/6JCya-Cfap157em1/Cya
Common Name:
Cfap157-KO
Product ID:
S-KO-06128
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cfap157-KO
Strain ID
KOCMP-227736-Cfap157-B6J-VA
Gene Name
Product ID
S-KO-06128
Gene Alias
1700019L03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cfap157em1/Cya mice (Catalog S-KO-06128) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102813
NCBI RefSeq
NM_025619
Target Region
Exon 1~8
Size of Effective Region
~7.5 kb
Detailed Document
Overview of Gene Research
Cfap157 is a gene that functions as a downstream effector of FOXJ1. It is specifically required for flagellum morphogenesis and sperm motility. Its expression is restricted to motile ciliated tissues in mouse and Xenopus in a FOXJ1-dependent manner. The protein localizes to basal bodies and interacts with tubulin and CEP350. Studies on Cfap157 in model organisms can help understand its in-vivo functions [1].
In Cfap157 knockout mice, although the overall appearance is normal, homozygous males are infertile. Spermatozoa from these mice show impaired motility, along with abnormal phenotypes such as axonemal loops, supernumerary axonemal profiles with ectopic accessory structures, excess cytoplasm, and clustered mitochondria in the mid-piece regions, and defective axonemes along the flagella. This indicates that Cfap157 is essential for spermiogenesis, suppressing the formation of supernumerary axonemes and ensuring correct sperm ultrastructure [1].
In conclusion, Cfap157 is crucial for flagellum morphogenesis and sperm motility. The Cfap157 knockout mouse model has revealed its significance in male infertility, highlighting its essential role in maintaining proper sperm structure and function during spermiogenesis [1].
References:
1. Weidemann, Marina, Schuster-Gossler, Karin, Stauber, Michael, Ueffing, Marius, Gossler, Achim. . CFAP157 is a murine downstream effector of FOXJ1 that is specifically required for flagellum morphogenesis and sperm motility. In Development (Cambridge, England), 143, 4736-4748. doi:. https://pubmed.ncbi.nlm.nih.gov/27965440/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen