C57BL/6NCya-Ppp2r5eem1/Cya
Common Name:
Ppp2r5e-KO
Product ID:
S-KO-08669
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ppp2r5e-KO
Strain ID
KOCMP-26932-Ppp2r5e-B6N-VA
Gene Name
Product ID
S-KO-08669
Gene Alias
4633401M22Rik; B56beta
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ppp2r5eem1/Cya mice (Catalog S-KO-08669) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021447
NCBI RefSeq
NM_012024
Target Region
Exon 3
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
Ppp2r5e, encoding the PP2A regulatory subunit B56ε, belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A (PP2A) is crucial for cellular signalling, playing a pivotal role in brain function and development. Ppp2r5e may be involved in regulating microtubule organization by interacting with microtubule crosslinking factor 1 (MTCL1) [3].
In cancer research, miR-19b directly negatively regulates Ppp2r5e in colorectal cancer (CRC) cells. Downregulation of Ppp2r5e mediates miR-19b-induced oncogenic effects, increasing cell viability, colonosphere formation, and cell migration, and also mediates miR-19b-induced 5-FU sensitivity in CRC cells [1]. In gastric cancer, miR-23a downregulates Ppp2r5e, and overexpression of Ppp2r5e reverses the negative effects of miR-23a on cell growth and apoptosis [2]. Downregulation of Ppp2r5e is also a common event in acute myeloid leukemia, affecting the oncogenic potential of leukemic cells [4]. In addition, a genetic polymorphism (rs1255722AA) in Ppp2r5e is associated with an increased risk of lung cancer in Chinese populations, and lung cancer tissues with this variant genotype have a lower mRNA level of Ppp2r5e [6]. In Alzheimer's disease, ApoE transcriptionally represses Ppp2r5e, reducing protein expression and disrupting the PPP2R5E-PP2AC complex, leading to decreased PP2A activity and potentially elevated Tau phosphorylation [5].
In conclusion, Ppp2r5e is a key regulatory subunit of PP2A, involved in important cellular signalling processes. Its dysregulation is associated with multiple diseases, especially cancers, where it may act as a tumour suppressor. Studies on Ppp2r5e contribute to understanding the pathogenesis of these diseases and may provide potential therapeutic targets.
References:
1. Santos, Andrea, Cristóbal, Ion, Caramés, Cristina, Rojo, Federico, García-Foncillas, Jesús. 2023. Deregulation of the miR-19b/PPP2R5E Signaling Axis Shows High Functional Impact in Colorectal Cancer Cells. In International journal of molecular sciences, 24, . doi:10.3390/ijms24097779. https://pubmed.ncbi.nlm.nih.gov/37175484/
2. Liu, Xue, Liu, Qian, Fan, Yajie, Liu, Min, Tang, Hua. 2014. Downregulation of PPP2R5E expression by miR-23a suppresses apoptosis to facilitate the growth of gastric cancer cells. In FEBS letters, 588, 3160-9. doi:10.1016/j.febslet.2014.05.068. https://pubmed.ncbi.nlm.nih.gov/24997345/
3. Hyodo, Toshinori, Ito, Satoko, Asano-Inami, Eri, Chen, Dan, Senga, Takeshi. . A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1. In The FEBS journal, 283, 3662-3671. doi:10.1111/febs.13835. https://pubmed.ncbi.nlm.nih.gov/27521566/
4. Cristóbal, Ion, Cirauqui, Cristina, Castello-Cros, Remedios, Calasanz, María J, Odero, María D. 2013. Downregulation of PPP2R5E is a common event in acute myeloid leukemia that affects the oncogenic potential of leukemic cells. In Haematologica, 98, e103-4. doi:10.3324/haematol.2013.084731. https://pubmed.ncbi.nlm.nih.gov/23812941/
5. Theendakara, Veena, Bredesen, Dale E, Rao, Rammohan V. 2017. Downregulation of protein phosphatase 2A by apolipoprotein E: Implications for Alzheimer's disease. In Molecular and cellular neurosciences, 83, 83-91. doi:10.1016/j.mcn.2017.07.002. https://pubmed.ncbi.nlm.nih.gov/28720530/
6. Yang, Rongrong, Yang, Lei, Qiu, Fuman, Zhou, Yifeng, Lu, Jiachun. 2013. Functional genetic polymorphisms in PP2A subunit genes confer increased risks of lung cancer in southern and eastern Chinese. In PloS one, 8, e77285. doi:10.1371/journal.pone.0077285. https://pubmed.ncbi.nlm.nih.gov/24204789/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen