C57BL/6JCya-Tas2r131em1/Cya
Common Name:
Tas2r131-KO
Product ID:
S-KO-09955
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Tas2r131-KO
Strain ID
KOCMP-387356-Tas2r131-B6J-VA
Gene Name
Product ID
S-KO-09955
Gene Alias
T2R31; Tas2r31; mGR31; mt2r61
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tas2r131em1/Cya mice (Catalog S-KO-09955) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000082085
NCBI RefSeq
NM_207030
Target Region
Exon 1
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Tas2r131 is a type 2 taste receptor, belonging to the large family of G protein-coupled receptors. It is primarily involved in recognizing bitter-tasting compounds, which are often aversive to vertebrates. The Tas2r131-mediated bitter taste perception is part of the body's defense mechanism against potentially harmful substances [1]. Genetic models, such as gene-targeted mice, are valuable tools for studying Tas2r131 as they allow for the manipulation and visualization of Tas2r131-expressing cells in vivo [1].
In gene-targeted mouse studies, the generation of Tas2r131-specific Cre knock-in mouse strains has enabled the visualization of Tas2r131-expressing cells in gustatory and nongustatory tissues. These cells were found in several nongustatory tissues like lung, trachea, ovary, ganglia, and brain, as well as in taste buds, mainly in foliate and vallate papillae. A subset of colonic goblet cells, deep-crypt Paneth cells in the ileum, cholinergic cells in the thymic medulla, and cells in tracheal and laryngeal glandular ducts also express Tas2r131. Additionally, Tas2r131 was identified among the receptors in the mouse Grueneberg ganglion involved in detecting danger-related volatile compounds [1,2,3,4,5,6].
In conclusion, Tas2r131 plays a crucial role in bitter taste perception, which is important for the body's defense against harmful substances. The use of KO/CKO mouse models has revealed its presence and potential functions in various gustatory and nongustatory tissues, suggesting its involvement in multiple physiological processes, although further research is needed to fully understand its implications in disease conditions.
References:
1. Voigt, Anja, Hübner, Sandra, Döring, Linda, Boehm, Ulrich, Meyerhof, Wolfgang. 2015. Cre-Mediated Recombination in Tas2r131 Cells-A Unique Way to Explore Bitter Taste Receptor Function Inside and Outside of the Taste System. In Chemical senses, 40, 627-39. doi:10.1093/chemse/bjv049. https://pubmed.ncbi.nlm.nih.gov/26377344/
2. Soultanova, Aichurek, Voigt, Anja, Chubanov, Vladimir, Boehm, Ulrich, Kummer, Wolfgang. 2015. Cholinergic chemosensory cells of the thymic medulla express the bitter receptor Tas2r131. In International immunopharmacology, 29, 143-7. doi:10.1016/j.intimp.2015.06.005. https://pubmed.ncbi.nlm.nih.gov/26102274/
3. Prandi, Simone, Bromke, Marta, Hübner, Sandra, Meyerhof, Wolfgang, Behrens, Maik. 2013. A subset of mouse colonic goblet cells expresses the bitter taste receptor Tas2r131. In PloS one, 8, e82820. doi:10.1371/journal.pone.0082820. https://pubmed.ncbi.nlm.nih.gov/24367558/
4. Prandi, Simone, Voigt, Anja, Meyerhof, Wolfgang, Behrens, Maik. 2017. Expression profiling of Tas2r genes reveals a complex pattern along the mouse GI tract and the presence of Tas2r131 in a subset of intestinal Paneth cells. In Cellular and molecular life sciences : CMLS, 75, 49-65. doi:10.1007/s00018-017-2621-y. https://pubmed.ncbi.nlm.nih.gov/28801754/
5. Moine, Fabian, Brechbühl, Julien, Nenniger Tosato, Monique, Beaumann, Manon, Broillet, Marie-Christine. 2018. Alarm pheromone and kairomone detection via bitter taste receptors in the mouse Grueneberg ganglion. In BMC biology, 16, 12. doi:10.1186/s12915-017-0479-y. https://pubmed.ncbi.nlm.nih.gov/29347925/
6. Krasteva-Christ, G, Soultanova, A, Schütz, B, Weihe, E, Kummer, W. 2015. Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts. In International immunopharmacology, 29, 158-65. doi:10.1016/j.intimp.2015.05.028. https://pubmed.ncbi.nlm.nih.gov/26033492/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen