C57BL/6NCya-Asb2em1/Cya
Common Name:
Asb2-KO
Product ID:
S-KO-11475
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Asb2-KO
Strain ID
KOCMP-65256-Asb2-B6N-VA
Gene Name
Product ID
S-KO-11475
Gene Alias
1110008E15Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Asb2em1/Cya mice (Catalog S-KO-11475) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021617
NCBI RefSeq
NM_023049
Target Region
Exon 3~6
Size of Effective Region
~4.9 kb
Detailed Document
Overview of Gene Research
Asb2, short for ankyrin repeat and SOCS Box containing 2, is a gene encoding a protein that functions as a key subunit of a Cullin5 Ub ligase complex [3]. It is involved in the ubiquitin-proteasome system, which is crucial for the selective degradation of proteins. Asb2 is associated with pathways such as BMP signalling, NF-κB pathway, and is important in processes like cardiogenesis, hematopoietic cell differentiation, and NK cell migration [1,2,4]. Genetic models, especially KO/CKO mouse models, have been instrumental in studying its functions.
In KO mouse models, deletion of Asb2 leads to embryonic lethality, heart defects like double outlet right ventricle (DORV), and placental defects [3,6]. In zebrafish, knockdown of Asb2 results in a thinned ventricular wall and dilated ventricle, which can be rescued by simultaneous knockdown of Smad9, indicating its role in cardiogenesis through regulation of BMP signalling [1]. In GCB DLBCL cell lines, down-regulation of Asb2 is toxic and induces NF-κB inhibition, suggesting its importance in sustaining NF-κB pathway activation in this type of lymphoma [2]. In NK cells, knockdown of ASB2 inhibits migration, showing its role in regulating NK cell migration [4]. In leukemia cells, knockdown of endogenous ASB2 delays retinoic acid-induced differentiation and filamin degradation [5].
In conclusion, Asb2 plays essential roles in multiple biological processes, particularly in cardiogenesis, cell differentiation, and immune cell function. The use of KO/CKO mouse models has significantly contributed to understanding its functions in heart development-related diseases like DORV and in cancer-related processes such as lymphoma development and leukemia cell differentiation [1,2,3,5,6].
References:
1. Min, Kyung-Duk, Asakura, Masanori, Shirai, Manabu, Takashima, Seiji, Kitakaze, Masafumi. 2021. ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis. In Scientific reports, 11, 23056. doi:10.1038/s41598-021-02390-0. https://pubmed.ncbi.nlm.nih.gov/34845242/
2. Sartori, Giulio, Napoli, Sara, Cascione, Luciano, Thome, Margot, Bertoni, Francesco. 2021. ASB2 is a direct target of FLI1 that sustains NF-κB pathway activation in germinal center-derived diffuse large B-cell lymphoma. In Journal of experimental & clinical cancer research : CR, 40, 357. doi:10.1186/s13046-021-02159-3. https://pubmed.ncbi.nlm.nih.gov/34763718/
3. Yamak, Abir, Hu, Dongjian, Mittal, Nikhil, Ellinor, Patrick T, Domian, Ibrahim J. 2020. Loss of Asb2 Impairs Cardiomyocyte Differentiation and Leads to Congenital Double Outlet Right Ventricle. In iScience, 23, 100959. doi:10.1016/j.isci.2020.100959. https://pubmed.ncbi.nlm.nih.gov/32179481/
4. Shin, June Ho, Moreno-Nieves, Uriel Y, Zhang, Luhua H, Mace, Emily M, Sunwoo, John B. 2021. AHR Regulates NK Cell Migration via ASB2-Mediated Ubiquitination of Filamin A. In Frontiers in immunology, 12, 624284. doi:10.3389/fimmu.2021.624284. https://pubmed.ncbi.nlm.nih.gov/33717133/
5. Heuzé, Mélina L, Lamsoul, Isabelle, Baldassarre, Massimiliano, Calderwood, David A, Lutz, Pierre G. 2008. ASB2 targets filamins A and B to proteasomal degradation. In Blood, 112, 5130-40. doi:10.1182/blood-2007-12-128744. https://pubmed.ncbi.nlm.nih.gov/18799729/
6. Park, Seul Gi, Kim, Eun-Kyoung, Nam, Ki-Hoan, Lee, Beom Jun, Nam, Sang-Yoon. 2021. Heart defects and embryonic lethality in Asb2 knock out mice correlate with placental defects. In Cells & development, 165, 203663. doi:10.1016/j.cdev.2021.203663. https://pubmed.ncbi.nlm.nih.gov/33993984/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen