Rbm25, an RNA-binding protein, is involved in multiple biological processes such as apoptosis, inflammation, and alternative splicing regulation. It is associated with pathways like endoplasmic reticulum stress, histone modification-related metabolic pathways, and is crucial for maintaining normal cellular functions and homeostasis. Genetic models, especially gene knockout models, have been instrumental in understanding its functions [1-10].

In an inducible Tet2 knockout mouse model, knocking down Rbm25 accelerated the expansion of Tet2-knockout hematopoietic cells both in vitro and in vivo, suggesting that Rbm25 underlies heterogeneous preleukemic clonal expansion [3]. In macrophage-specific Rbm25-deficient mice, there was increased pro-inflammatory mediator production and tissue inflammatory injury, indicating Rbm25's role in restraining inflammation [1]. In colon cancer cells, Rbm25 ablation suppressed cell growth both in vitro and in vivo by regulating the alternative splicing of MNK2 [2].

In conclusion, Rbm25 plays essential roles in apoptosis, inflammation, and tumor-related processes. The use of gene knockout models, like those in mice, has been key in revealing its functions in preleukemic clonal expansion, inflammatory regulation, and cancer-related biological processes, providing potential therapeutic targets for ischemic heart failure, rheumatoid arthritis, colon cancer, and other diseases [1-3, 9].

References:

1. Zhang, Yunkai, Gao, Ying, Wang, Yujia, Zhan, Zhenzhen, Liu, Xingguang. 2024. RBM25 is required to restrain inflammation via ACLY RNA splicing-dependent metabolism rewiring. In Cellular & molecular immunology, 21, 1231-1250. doi:10.1038/s41423-024-01212-3. https://pubmed.ncbi.nlm.nih.gov/39251781/

2. Zhi, Lili, Chen, Chaoqun, Zhang, Ge, Zhang, Wenjing, Wang, Yang. 2024. RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2. In Science China. Life sciences, 67, 2186-2197. doi:10.1007/s11427-023-2582-x. https://pubmed.ncbi.nlm.nih.gov/39110401/

3. Bramlett, Charles, Eerdeng, Jiya, Jiang, Du, Nogalska, Anna, Lu, Rong. . RNA splicing factor Rbm25 underlies heterogeneous preleukemic clonal expansion in mice. In Blood, 141, 2961-2972. doi:10.1182/blood.2023019620. https://pubmed.ncbi.nlm.nih.gov/36947858/