Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Ndc80em1/Cya
Common Name:
Ndc80-KO
Product ID:
S-KO-12077
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ndc80-KO
Strain ID
KOCMP-67052-Ndc80-B6N-VA
Gene Name
Ndc80
Product ID
S-KO-12077
Gene Alias
2610020P18Rik; HEC; HEC1; Kntc2
Background
C57BL/6NCya
NCBI ID
67052
Modification
Conventional knockout
Chromosome
17
Phenotype
MGI:1914302
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ndc80em1/Cya mice (Catalog S-KO-12077) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024851
NCBI RefSeq
NM_023294
Target Region
Exon 4~7
Size of Effective Region
~3.6 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ndc80, a key component of the kinetochore-microtubule attachment machinery in eukaryotic cells, is essential for successful mitotic cell division [1,3,4,6,7]. The Ndc80 complex, with a small disordered region in the Hec1 subunit (the N-terminal "tail" domain), mediates the formation of correct attachments between chromosomes and spindle microtubules [1]. These attachments need to be stable yet regulated for timely, error-free mitotic progression. The complex is also involved in pathways like chromosome segregation and spindle checkpoint signaling [3,6,7].

In triple-negative breast cancer (TNBC), Ndc80 is considered a cisplatin-resistant gene. Its expression is upregulated in cisplatin-resistant cells and TNBC tissues compared to normal tissues. Overexpression of Ndc80 promotes TNBC cell viability, proliferation, and cisplatin resistance [2]. In glioma, the Hec1 component of the Ndc80 complex is overexpressed, indicating poor prognosis. It promotes cell proliferation, DNA replication and repair, and macrophage polarization from M1 to M2 phenotype, contributing to an immunosuppressive tumor microenvironment [5].

In conclusion, Ndc80 is crucial for mitotic cell division through its role in kinetochore-microtubule attachments. Studies in TNBC and glioma suggest its potential as a therapeutic target. Understanding Ndc80's function helps in unravelling the mechanisms of cancer drug resistance and tumor progression, providing new directions for cancer treatment [2,5].

References:

1. Wimbish, Robert T, DeLuca, Jennifer G. 2020. Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface. In Frontiers in cell and developmental biology, 8, 43. doi:10.3389/fcell.2020.00043. https://pubmed.ncbi.nlm.nih.gov/32161753/

2. Li, Jing, Xu, Xiaoqin, Peng, Xiting. 2022. NDC80 Enhances Cisplatin-resistance in Triple-negative Breast Cancer. In Archives of medical research, 53, 378-387. doi:10.1016/j.arcmed.2022.03.003. https://pubmed.ncbi.nlm.nih.gov/35346500/

3. Chen, Jingxun, Ünal, Elçin. 2021. Meiotic regulation of the Ndc80 complex composition and function. In Current genetics, 67, 511-518. doi:10.1007/s00294-021-01174-3. https://pubmed.ncbi.nlm.nih.gov/33745061/

4. Ustinov, N B, Korshunova, A V, Gudimchuk, N B. . Protein Complex NDC80: Properties, Functions, and Possible Role in Pathophysiology of Cell Division. In Biochemistry. Biokhimiia, 85, 448-462. doi:10.1134/S0006297920040057. https://pubmed.ncbi.nlm.nih.gov/32569552/

5. Ye, Weijie, Liang, Xisong, Chen, Ge, Cheng, Quan, Chen, Xiaoping. . NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment. In CNS neuroscience & therapeutics, 30, e14850. doi:10.1111/cns.14850. https://pubmed.ncbi.nlm.nih.gov/39021287/

6. Tooley, John, Stukenberg, P Todd. . The Ndc80 complex: integrating the kinetochore's many movements. In Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 19, 377-91. doi:10.1007/s10577-010-9180-5. https://pubmed.ncbi.nlm.nih.gov/21311965/

7. Ciferri, Claudio, Musacchio, Andrea, Petrovic, Arsen. 2007. The Ndc80 complex: hub of kinetochore activity. In FEBS letters, 581, 2862-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17521635/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest