C57BL/6NCya-Ndc80em1/Cya
Common Name:
Ndc80-KO
Product ID:
S-KO-12077
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ndc80-KO
Strain ID
KOCMP-67052-Ndc80-B6N-VA
Gene Name
Product ID
S-KO-12077
Gene Alias
2610020P18Rik; HEC; HEC1; Kntc2
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ndc80em1/Cya mice (Catalog S-KO-12077) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024851
NCBI RefSeq
NM_023294
Target Region
Exon 4~7
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Ndc80, a key component of the kinetochore-microtubule attachment machinery in eukaryotic cells, is essential for successful mitotic cell division [1,3,4,6,7]. The Ndc80 complex, with a small disordered region in the Hec1 subunit (the N-terminal "tail" domain), mediates the formation of correct attachments between chromosomes and spindle microtubules [1]. These attachments need to be stable yet regulated for timely, error-free mitotic progression. The complex is also involved in pathways like chromosome segregation and spindle checkpoint signaling [3,6,7].
In triple-negative breast cancer (TNBC), Ndc80 is considered a cisplatin-resistant gene. Its expression is upregulated in cisplatin-resistant cells and TNBC tissues compared to normal tissues. Overexpression of Ndc80 promotes TNBC cell viability, proliferation, and cisplatin resistance [2]. In glioma, the Hec1 component of the Ndc80 complex is overexpressed, indicating poor prognosis. It promotes cell proliferation, DNA replication and repair, and macrophage polarization from M1 to M2 phenotype, contributing to an immunosuppressive tumor microenvironment [5].
In conclusion, Ndc80 is crucial for mitotic cell division through its role in kinetochore-microtubule attachments. Studies in TNBC and glioma suggest its potential as a therapeutic target. Understanding Ndc80's function helps in unravelling the mechanisms of cancer drug resistance and tumor progression, providing new directions for cancer treatment [2,5].
References:
1. Wimbish, Robert T, DeLuca, Jennifer G. 2020. Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface. In Frontiers in cell and developmental biology, 8, 43. doi:10.3389/fcell.2020.00043. https://pubmed.ncbi.nlm.nih.gov/32161753/
2. Li, Jing, Xu, Xiaoqin, Peng, Xiting. 2022. NDC80 Enhances Cisplatin-resistance in Triple-negative Breast Cancer. In Archives of medical research, 53, 378-387. doi:10.1016/j.arcmed.2022.03.003. https://pubmed.ncbi.nlm.nih.gov/35346500/
3. Chen, Jingxun, Ünal, Elçin. 2021. Meiotic regulation of the Ndc80 complex composition and function. In Current genetics, 67, 511-518. doi:10.1007/s00294-021-01174-3. https://pubmed.ncbi.nlm.nih.gov/33745061/
4. Ustinov, N B, Korshunova, A V, Gudimchuk, N B. . Protein Complex NDC80: Properties, Functions, and Possible Role in Pathophysiology of Cell Division. In Biochemistry. Biokhimiia, 85, 448-462. doi:10.1134/S0006297920040057. https://pubmed.ncbi.nlm.nih.gov/32569552/
5. Ye, Weijie, Liang, Xisong, Chen, Ge, Cheng, Quan, Chen, Xiaoping. . NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment. In CNS neuroscience & therapeutics, 30, e14850. doi:10.1111/cns.14850. https://pubmed.ncbi.nlm.nih.gov/39021287/
6. Tooley, John, Stukenberg, P Todd. . The Ndc80 complex: integrating the kinetochore's many movements. In Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 19, 377-91. doi:10.1007/s10577-010-9180-5. https://pubmed.ncbi.nlm.nih.gov/21311965/
7. Ciferri, Claudio, Musacchio, Andrea, Petrovic, Arsen. 2007. The Ndc80 complex: hub of kinetochore activity. In FEBS letters, 581, 2862-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17521635/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen