C57BL/6JCya-Ghrlem1/Cya
Common Name:
Ghrl-KO
Product ID:
S-KO-16189
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ghrl-KO
Strain ID
KOCMP-58991-Ghrl-B6J-VB
Gene Name
Product ID
S-KO-16189
Gene Alias
2210006E23Rik; Ghr; MTLRP; MTLRPAP; m46
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ghrlem1/Cya mice (Catalog S-KO-16189) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064993
NCBI RefSeq
NM_021488
Target Region
Exon 4
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
GHRL, which encodes the ghrelin prepropeptide, is a gene with significant importance in regulating appetite and energy balance in the human body [2]. Ghrelin, the protein it encodes, may also be involved in various biological processes such as carcinogenesis and immune regulation [2]. Genetic studies of GHRL can offer insights into its functions and associated health implications.
In terms of disease associations, the GHRL gene Leu72Met polymorphism is significantly correlated with an increased risk of type 2 diabetes mellitus (T2DM), especially in the Chinese population. Carriers of the Met72 allele may be more susceptible to T2DM [1]. In gastric cancer, GHRL expression in cancer samples is lower than in normal samples, and high GHRL expression is correlated with sample type, cancer stage, worse survival, and immune cell infiltrates, making it a potential prognostic biomarker [2]. In obesity, the presence of the T allele of the GHRL (rs696217) polymorphism in the Ukrainian population indicates an increased risk of obesity development, and it also affects adiponectin and resistin levels [3]. However, no significant association was found between GHRL polymorphisms and prostate cancer [4].
In conclusion, GHRL plays a crucial role in energy regulation and is associated with diseases like T2DM, gastric cancer, and obesity. Studies on its genetic polymorphisms, as well as expression levels in different diseases, contribute to understanding its functions and potential as a biomarker or therapeutic target in these disease areas.
References:
1. Li, Yan-Yan, Lu, Xin-Zheng, Yang, Xin-Xing, Kim, Hyun Jun, Yang, Zhi-Jian. 2019. GHRL Gene Leu72Met Polymorphism and Type 2 Diabetes Mellitus: A Meta-Analysis Involving 8,194 Participants. In Frontiers in endocrinology, 10, 559. doi:10.3389/fendo.2019.00559. https://pubmed.ncbi.nlm.nih.gov/31440212/
2. Wang, Jinyun, Liu, Dingwei, Xie, Yong. 2023. GHRL as a prognostic biomarker correlated with immune infiltrates and progression of precancerous lesions in gastric cancer. In Frontiers in oncology, 13, 1142017. doi:10.3389/fonc.2023.1142017. https://pubmed.ncbi.nlm.nih.gov/37469414/
3. Prodan, Andrii, Dzubanovsky, Ihor, Kamyshnyi, Oleksandr, Grytsenko, Stepan, Voloshyn, Stanislava. 2023. Effect of the GHRL gene (rs696217) polymorphism on the metabolic disorders in patients with obesity in the Ukrainian population. In Endocrine regulations, 57, 173-182. doi:10.2478/enr-2023-0021. https://pubmed.ncbi.nlm.nih.gov/37715984/
4. Merabet, Nesrine, Ramoz, Nicolas, Boulmaiz, Amel, Tolle, Virginie, Berredjem, Hajira. 2023. SNPs-Panel Polymorphism Variations in GHRL and GHSR Genes Are Not Associated with Prostate Cancer. In Biomedicines, 11, . doi:10.3390/biomedicines11123276. https://pubmed.ncbi.nlm.nih.gov/38137497/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen