C57BL/6JCya-Prss21em1/Cya
Common Name:
Prss21-KO
Product ID:
S-KO-16851
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prss21-KO
Strain ID
KOCMP-57256-Prss21-B6J-VB
Gene Name
Product ID
S-KO-16851
Gene Alias
1700023E12Rik; TESP5; mT4; testisin
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prss21em1/Cya mice (Catalog S-KO-16851) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024928
NCBI RefSeq
NM_020487.4
Target Region
Exon 4
Size of Effective Region
~0.3 kb
Detailed Document
Overview of Gene Research
Prss21, also known as testisin, is a gene encoding a glycosyl-phosphatidylinositol-linked serine protease. It is involved in multiple biological functions, including sperm cell maturation, angiogenesis regulation, and may play a role in tumor metastasis-related processes [1,2,3]. In the context of angiogenesis, it appears to regulate vascular integrity, and in sperm-related functions, it impacts sperm motility, morphology, and the ability to fertilize oocytes.
Genetic models, especially knockout mouse models, have been crucial in understanding Prss21 function. Prss21-deficient mice show increased vascular permeability and hemorrhagic phenotypes during luteal angiogenesis, suggesting its importance in maintaining vascular integrity [2]. In sperm-related studies, Prss21-deficient spermatozoa have decreased motility, abnormal tails, and impaired fertilizing ability, highlighting its role in epididymal sperm cell maturation [3]. Additionally, in ovarian cancer, increased testisin expression (from Prss21) inhibits intra-peritoneal tumor seeding, ascites accumulation, and metastatic tumor burden in a murine xenograft model [1].
In summary, Prss21 plays essential roles in sperm-related functions and angiogenesis, and its dysregulation may be associated with ovarian cancer metastasis. The use of KO mouse models has been instrumental in revealing these functions, providing valuable insights into potential therapeutic targets for treating infertility-related issues and ovarian cancer.
References:
1. Conway, Gregory D, Buzza, Marguerite S, Martin, Erik W, Pawar, Nisha R, Antalis, Toni M. 2019. PRSS21/testisin inhibits ovarian tumor metastasis and antagonizes proangiogenic angiopoietins ANG2 and ANGPTL4. In Journal of molecular medicine (Berlin, Germany), 97, 691-709. doi:10.1007/s00109-019-01763-3. https://pubmed.ncbi.nlm.nih.gov/30911775/
2. Peroutka, Raymond J, Buzza, Marguerite S, Mukhopadhyay, Subhradip, Driesbaugh, Kathryn H, Antalis, Toni M. 2020. Testisin/Prss21 deficiency causes increased vascular permeability and a hemorrhagic phenotype during luteal angiogenesis. In PloS one, 15, e0234407. doi:10.1371/journal.pone.0234407. https://pubmed.ncbi.nlm.nih.gov/32511276/
3. Netzel-Arnett, Sarah, Bugge, Thomas H, Hess, Rex A, Kay, Graham F, Antalis, Toni M. 2009. The glycosylphosphatidylinositol-anchored serine protease PRSS21 (testisin) imparts murine epididymal sperm cell maturation and fertilizing ability. In Biology of reproduction, 81, 921-32. doi:10.1095/biolreprod.109.076273. https://pubmed.ncbi.nlm.nih.gov/19571264/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen