C57BL/6JCya-Dgkzem1/Cya
Common Name:
Dgkz-KO
Product ID:
S-KO-17191
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dgkz-KO
Strain ID
KOCMP-104418-Dgkz-B6J-VB
Gene Name
Product ID
S-KO-17191
Gene Alias
E130307B02Rik; F730209L11Rik; mDGK[z]
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dgkzem1/Cya mice (Catalog S-KO-17191) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028667
NCBI RefSeq
NM_138306
Target Region
Exon 2~4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Dgkz, or diacylglycerol kinase ζ, is a diacylglycerol kinase that metabolizes diacylglycerol to yield phosphatidic acid. It is involved in lipid signaling pathways, which are fundamental to neural activities, memory, and learning [3]. It also seems to be associated with several biological processes and disease-related pathways, such as the transforming growth factor β (TGFβ) signaling pathway [1].
In triple-negative breast cancer, knockout of Dgkz in cell lines significantly inhibited metastatic behaviors in vitro and in vivo, indicating its role as a prometastatic gene, possibly by promoting epithelial-mesenchymal transition via the TGFβ signaling pathway [1]. In osteosarcoma, silence of Dgkz by shRNA hampered cell growth and promoted apoptosis in vitro, and its knockout suppressed xenograft tumor proliferation in vivo. It might act as an oncogene through its possible interaction with ERK1/2 and MYC pathway [2]. In cervical cancer, down-regulation of Dgkz repressed cell proliferation, boosted apoptosis, and induced cell cycle arrest, and its knockdown restrained tumor growth in xenograft mice [4]. In human acute myeloid leukemia HL-60 cells, knockdown of Dgkz induced apoptosis and G2/M phase arrest through the MAPK/survivin/caspase pathway [5].
In conclusion, Dgkz is involved in multiple disease-related biological processes. Gene knockout and knockdown studies in various cell lines and in vivo models have revealed its role in promoting tumor metastasis, cell proliferation, and suppressing apoptosis in cancers such as triple-negative breast cancer, osteosarcoma, cervical cancer, and acute myeloid leukemia. These findings suggest Dgkz could be a potential target for cancer treatment and a prognostic indicator [1,2,4,5].
References:
1. Zhao, Yuanyuan, Sun, Hefen, Li, Xuan, Hou, Yifeng, Jin, Wei. 2022. DGKZ promotes TGFβ signaling pathway and metastasis in triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFβR2. In Cell death & disease, 13, 105. doi:10.1038/s41419-022-04537-x. https://pubmed.ncbi.nlm.nih.gov/35115500/
2. Yu, Wenxi, Tang, Lina, Lin, Feng, Yao, Yang, Shen, Zan. 2019. DGKZ Acts as a Potential Oncogene in Osteosarcoma Proliferation Through Its Possible Interaction With ERK1/2 and MYC Pathway. In Frontiers in oncology, 8, 655. doi:10.3389/fonc.2018.00655. https://pubmed.ncbi.nlm.nih.gov/30662872/
3. Alinaghi, Somayeh, Alehabib, Elham, Johari, Amir Hossein, Darvish, Hossein, Ghaedi, Hamid. 2019. Expression analysis and genotyping of DGKZ: a GWAS-derived risk gene for schizophrenia. In Molecular biology reports, 46, 4105-4111. doi:10.1007/s11033-019-04860-1. https://pubmed.ncbi.nlm.nih.gov/31087244/
4. Liu, Keying, Xue, Biyun, Bai, Guiqin, Zhang, Wentao. . Downregulation of Diacylglycerol kinase zeta (DGKZ) suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest. In Bioengineered, 12, 1517-1529. doi:10.1080/21655979.2021.1918505. https://pubmed.ncbi.nlm.nih.gov/33926342/
5. Li, Hong, Dong, Changhu, Tian, Yanning, Bai, Yingying, Chao, Xu. . Knockdown of diacylglycerol kinase zeta (DGKZ) induces apoptosis and G2/M phase arrest in human acute myeloid leukemia HL-60 cells through MAPK/survivin/caspase pathway. In Die Pharmazie, 74, 418-422. doi:10.1691/ph.2019.9386. https://pubmed.ncbi.nlm.nih.gov/31288898/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen