C57BL/6JCya-Lrwd1em1/Cya
Common Name:
Lrwd1-KO
Product ID:
S-KO-17260
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lrwd1-KO
Strain ID
KOCMP-71735-Lrwd1-B6J-VB
Gene Name
Product ID
S-KO-17260
Gene Alias
1200011O22Rik; Orca
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lrwd1em1/Cya mice (Catalog S-KO-17260) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006301
NCBI RefSeq
NM_027891
Target Region
Exon 2~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Lrwd1, also known as ORCA, is a nuclear protein involved in multiple biological processes. It interacts with repressive histone marks via its WD40 domain, maintaining heterochromatin region silencing in mammalian cells. It also associates with the origin recognition complex (ORC), facilitating prereplication complex formation at late-replicating origins. Understanding its function is crucial as it likely plays important roles in various cellular and developmental processes [1,2,3,4].
Using a gene-trap approach to generate Lrwd1 knockout mice, it was found that Lrwd1 is ubiquitously expressed in the developing mouse embryo. Depletion of LRWD1 did not impact embryonic development, but the postnatal growth of homozygous mutants was retarded. In vitro, mouse embryonic fibroblasts (MEFs) lacking LRWD1 showed reduced proliferation. Also, the knockout of Lrwd1 in MEFs increased the expression of epigenetically silenced repetitive elements with minimal effect on protein-coding gene expression, suggesting Lrwd1 plays an important role in postnatal development by regulating cell proliferation, likely through modulating DNA replication [1].
In conclusion, Lrwd1 is essential for postnatal development by regulating cell proliferation, potentially through DNA replication modulation. The Lrwd1 knockout mouse model has been instrumental in uncovering these functions, providing insights into its role in normal development and potentially in diseases related to abnormal cell proliferation.
References:
1. Kang, Tze Zhen Evangeline, Wan, Yi Ching Esther, Zhang, Zhiguo, Chan, Kui Ming. 2022. Lrwd1 impacts cell proliferation and the silencing of repetitive DNA elements. In Genesis (New York, N.Y. : 2000), 60, e23475. doi:10.1002/dvg.23475. https://pubmed.ncbi.nlm.nih.gov/35451548/
2. Hsu, Rosaline Y C, Lin, Yo-Chuen, Redon, Christophe, Prasanth, Kannanganattu V, Prasanth, Supriya G. 2020. ORCA/LRWD1 Regulates Homologous Recombination at ALT-Telomeres by Modulating Heterochromatin Organization. In iScience, 23, 101038. doi:10.1016/j.isci.2020.101038. https://pubmed.ncbi.nlm.nih.gov/32344376/
3. Sahu, Sumon, Ekundayo, Babatunde E, Kumar, Ashish, Bleichert, Franziska. 2023. A dual role for the chromatin reader ORCA/LRWD1 in targeting the origin recognition complex to chromatin. In The EMBO journal, 42, e114654. doi:10.15252/embj.2023114654. https://pubmed.ncbi.nlm.nih.gov/37551430/
4. Giri, Sumanprava, Prasanth, Supriya G. 2016. Association of ORCA/LRWD1 with repressive histone methyl transferases mediates heterochromatin organization. In Nucleus (Austin, Tex.), 6, 435-41. doi:10.1080/19491034.2015.1102814. https://pubmed.ncbi.nlm.nih.gov/26765314/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen