C57BL/6JCya-Gcn1em1/Cya
Common Name:
Gcn1-KO
Product ID:
S-KO-17505
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gcn1-KO
Strain ID
KOCMP-231659-Gcn1-B6J-VB
Gene Name
Product ID
S-KO-17505
Gene Alias
4932409G22; G431004K08Rik; GCN1L; Gcn1l1; mKIAA0219
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gcn1em1/Cya mice (Catalog S-KO-17505) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064454
NCBI RefSeq
NM_172719
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Gcn1 is a highly conserved protein across eukaryotes. As an upstream activator of protein kinase GCN2, it is pivotal in integrated stress responses like amino acid starvation and oxidative stress [1]. Gcn1 also acts as a ribosome collision sensor, contributing to the translation quality control pathway [1].
Gcn1 mutant mice lacking the GCN2 - binding domain suffer from growth retardation and postnatal lethality via GCN2 - independent mechanisms, while Gcn1 - null mice die early in embryonic development [3]. Inducible systemic Gcn1 deletion in adult mice using tamoxifen - inducible conditional knockout (CKO) mice led to transient body weight loss, along with decreases in liver weight, hepatic glycogen and lipid contents, blood glucose, non - esterified fatty acids, and visceral white adipose tissue weight [3]. This shows GCN1's involvement in hepatic lipid metabolism [3]. In prostate cancer, GCN1 is overexpressed and interacts with MIRO2. Targeting the MIRO2 - GCN1 axis may be a strategy to halt prostate cancer growth as MIRO2 is necessary for efficient GCN1 - mediated GCN2 kinase signaling [2].
In conclusion, Gcn1 is essential for stress responses and translation quality control. Studies using KO/CKO mouse models have revealed its role in growth, development, and metabolism - related processes, as well as in prostate cancer. Understanding Gcn1's functions provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Zheng, Xinying, Shen, Jinze, Jiang, Hongting, Du, Lihua, Duan, Shiwei. 2024. Exploring the multifaceted role of GCN1: Implications in cellular responses and disease pathogenesis. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 175, 116681. doi:10.1016/j.biopha.2024.116681. https://pubmed.ncbi.nlm.nih.gov/38705128/
2. Furnish, Madison, Boulton, Dillon P, Genther, Victoria, Cramer, Scott D, Caino, M Cecilia. . MIRO2 Regulates Prostate Cancer Cell Growth via GCN1-Dependent Stress Signaling. In Molecular cancer research : MCR, 20, 607-621. doi:10.1158/1541-7786.MCR-21-0374. https://pubmed.ncbi.nlm.nih.gov/34992146/
3. Liu, Jun, Kasai, Shuya, Tatara, Yota, Tomita, Hirofumi, Itoh, Ken. 2022. Inducible Systemic Gcn1 Deletion in Mice Leads to Transient Body Weight Loss upon Tamoxifen Treatment Associated with Decrease of Fat and Liver Glycogen Storage. In International journal of molecular sciences, 23, . doi:10.3390/ijms23063201. https://pubmed.ncbi.nlm.nih.gov/35328622/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen