C57BL/6NCya-Wtapem1/Cya
Common Name:
Wtap-KO
Product ID:
S-KO-17645
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Wtap-KO
Strain ID
KOCMP-60532-Wtap-B6N-VA
Gene Name
Product ID
S-KO-17645
Gene Alias
2810408K05Rik; 9430038B09Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Wtapem1/Cya mice (Catalog S-KO-17645) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000159551
NCBI RefSeq
NM_001379058
Target Region
Exon 4~5
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Wilms tumor 1-associated protein (WTAP) is a key regulatory subunit of the RNA N6-methyladenosine (m6A) methyltransferase complex [1,4]. The m6A modification, catalyzed by this complex, is a prevalent post-transcriptional RNA modification in eukaryotes, regulating diverse biological processes such as RNA splicing, cell proliferation, cell cycle, and embryonic development [3].
In hepatocytes, deficiency of Wtap leads to lipoatrophy and non-alcoholic steatohepatitis (NASH). Hepatic deletion of Wtap causes NASH due to increased lipolysis in white adipose tissue, enhanced hepatic free fatty acids uptake, and induced inflammation, mediated by IGFBP1, CD36, and cytochemokines like CCL2 respectively [2]. In zebrafish embryos, morpholino-mediated knockdown targeting WTAP caused tissue differentiation defects and increased apoptosis, indicating its crucial role in development [1].
In conclusion, WTAP is essential for the m6A RNA methylation modification, which is involved in multiple biological processes. Studies using gene-knockout models, such as Wtap-deficient mouse models, have revealed its significance in diseases like NASH, highlighting its potential as a therapeutic target for related diseases.
References：
1. Ping, Xiao-Li, Sun, Bao-Fa, Wang, Lu, Liu, Feng, Yang, Yun-Gui. 2014. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. In Cell research, 24, 177-89. doi:10.1038/cr.2014.3. https://pubmed.ncbi.nlm.nih.gov/24407421/
2. Li, Xinzhi, Ding, Kaixin, Li, Xueying, Chen, Xiao-Wei, Chen, Zheng. 2022. Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH). In Nature communications, 13, 4549. doi:10.1038/s41467-022-32163-w. https://pubmed.ncbi.nlm.nih.gov/35927268/
3. Fan, Yongfei, Li, Xinwei, Sun, Huihui, Zhu, Zheng, Yuan, Kai. 2022. Role of WTAP in Cancer: From Mechanisms to the Therapeutic Potential. In Biomolecules, 12, . doi:10.3390/biom12091224. https://pubmed.ncbi.nlm.nih.gov/36139062/
4. Huang, Qibo, Mo, Jie, Liao, Zhibin, Chen, Xiaoping, Zhang, Bixiang. 2022. The RNA m6A writer WTAP in diseases: structure, roles, and mechanisms. In Cell death & disease, 13, 852. doi:10.1038/s41419-022-05268-9. https://pubmed.ncbi.nlm.nih.gov/36207306/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen