C57BL/6JCya-Prkd1em1/Cya
Common Name:
Prkd1-KO
Product ID:
S-KO-17783
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Prkd1-KO
Strain ID
KOCMP-18760-Prkd1-B6J-VA
Gene Name
Product ID
S-KO-17783
Gene Alias
PKD; PKD1; Pkcm; Prkcm; nPKC-D1; nPKC-mu
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prkd1em1/Cya mice (Catalog S-KO-17783) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002765
NCBI RefSeq
NM_008858
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Prkd1, also known as PKCμ, encodes a serine/threonine protein kinase that is involved in a variety of fundamental cellular functions [4]. It is associated with pathways like the KRas-NF-κB pathway in pancreatic cancer, where the PRKD1 gene promoter is a target for oncogenic KRas signaling [2]. Prkd1 is important for biological processes such as cell-cell adhesion regulation in endothelial cells via the PKCμ/PRKD1-p38 MAPK-α-catenin signaling pathway [3].
In a Prkd1 transgenic mouse model (Prkd1em1) with deletion of exon 2 causing loss of function, homozygous deletion of Prkd1 is associated with complex congenital heart diseases (CHDs) like atrioventricular septal defects, bicuspid aortic and pulmonary valves, and is lethal. Even in heterozygotes, cardiac differences occur [4]. In osteocytes, inhibition or genetic deletion of Prkd1 in mice slows plasma membrane disruption (PMD) repair rate and impairs post-wounding cell survival. In vivo, Prkd1 conditional knockout (CKO) mice show blunted anabolic responses to tibial loading [1].
In conclusion, Prkd1 plays a pivotal role in heart development, the repair of plasma membrane disruptions in osteocytes, and is associated with CHDs. The study of Prkd1 through gene knockout and conditional knockout mouse models provides important insights into its functions in these biological processes and disease conditions.
References:
1. Tuladhar, Anik, Shaver, Joseph C, McGee, Wesley A, Hamrick, Mark W, McGee-Lawrence, Meghan E. 2024. Prkd1 regulates the formation and repair of plasma membrane disruptions (PMD) in osteocytes. In Bone, 186, 117147. doi:10.1016/j.bone.2024.117147. https://pubmed.ncbi.nlm.nih.gov/38866124/
2. Döppler, Heike, Panayiotou, Richard, Reid, Elizabeth M, Bastea, Ligia, Storz, Peter. 2016. The PRKD1 promoter is a target of the KRas-NF-κB pathway in pancreatic cancer. In Scientific reports, 6, 33758. doi:10.1038/srep33758. https://pubmed.ncbi.nlm.nih.gov/27649783/
3. Sharma, Deepti, Kaur, Geetika, Bisen, Shivantika, Ibrahim, Ahmed S, Singh, Nikhlesh K. 2023. IL-33 via PKCμ/PRKD1 Mediated α-Catenin Phosphorylation Regulates Endothelial Cell-Barrier Integrity and Ischemia-Induced Vascular Leakage. In Cells, 12, . doi:10.3390/cells12050703. https://pubmed.ncbi.nlm.nih.gov/36899839/
4. Waheed-Ullah, Qazi, Wilsdon, Anna, Abbad, Aseel, Brook, J David, Loughna, Siobhan. 2024. Effect of deletion of the protein kinase PRKD1 on development of the mouse embryonic heart. In Journal of anatomy, 245, 70-83. doi:10.1111/joa.14033. https://pubmed.ncbi.nlm.nih.gov/38419169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen