C57BL/6JCya-Itgamem1/Cya
Common Name:
Itgam-KO
Product ID:
S-KO-18240
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Itgam-KO
Strain ID
KOCMP-16409-Itgam-B6J-VB
Gene Name
Product ID
S-KO-18240
Gene Alias
CD11b/CD18; CR3; CR3A; Cd11b; F730045J24Rik; Ly-40; MAC1; Mac-1; Mac-1a
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgamem1/Cya mice (Catalog S-KO-18240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064821
NCBI RefSeq
NM_001082960
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Itgam, also known as Integrin Subunit α M, encodes the CD11b chain of the Mac-1 integrin. Mac-1 integrin is involved in cell adhesion, phagocytosis, and regulation of inflammatory cytokine production in macrophages. It plays a crucial role in the immune response and inflammation-related pathways [6].
In ischemic stroke, Itgam was identified as a critical gene. Its overexpression was found in patients, and its modulation influenced the secretion of inflammatory cytokines like IL-1β, IL-6, and TNF-α, and affected neuronal apoptosis, suggesting it may be a potential therapeutic target [1].
In systemic lupus erythematosus (SLE), multiple meta-analyses have shown a significant association between Itgam gene polymorphism (such as rs1143679) and SLE susceptibility in different ethnic populations. The A allele of rs1143679 was more prevalent in SLE patients [2,5,7].
In neutrophilic asthma, Itgam was identified as a key target to distinguish the disease from healthy/non-NA individuals, with a strong correlation between Itgam and macrophages [3].
In diabetic nephropathy and atherosclerosis, Itgam was found to be a key gene involved in basement membrane alterations, mainly expressed in macrophages, and may contribute to basement membrane lesions [4].
In Kawasaki disease, NFIL3 was found to aggravate human coronary artery endothelial cell injury by promoting Itgam transcription [8].
In conclusion, Itgam is essential in immune-related functions and inflammation-associated pathways. Its dysregulation is implicated in multiple diseases including ischemic stroke, SLE, neutrophilic asthma, diabetic nephropathy, atherosclerosis, and Kawasaki disease. Studies on Itgam, especially through gene-based models in vivo, have provided insights into the disease mechanisms, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Hou, Lei, Li, Zhongchen, Guo, Xiaoli, Zhang, Liyong, Li, Zefu. 2024. ITGAM is a critical gene in ischemic stroke. In Aging, 16, 6852-6867. doi:10.18632/aging.205729. https://pubmed.ncbi.nlm.nih.gov/38637126/
2. Fan, Y, Li, L-H, Pan, H-F, Sun, Z-Q, Ye, D-Q. . Association of ITGAM polymorphism with systemic lupus erythematosus: a meta-analysis. In Journal of the European Academy of Dermatology and Venereology : JEADV, 25, 271-5. doi:10.1111/j.1468-3083.2010.03776.x. https://pubmed.ncbi.nlm.nih.gov/20629846/
3. Yan, Qian, Liu, Zixing, Chen, Yujing, Zhan, Shaofeng, Huang, Xiufang. 2023. ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments. In Apoptosis : an international journal on programmed cell death, 29, 393-411. doi:10.1007/s10495-023-01914-5. https://pubmed.ncbi.nlm.nih.gov/37950848/
4. Lou, Yude, Li, Peng Hui, Liu, Xiao Qi, Chen, Chen Chen, Ma, Kun Ling. 2024. ITGAM-mediated macrophages contribute to basement membrane damage in diabetic nephropathy and atherosclerosis. In BMC nephrology, 25, 72. doi:10.1186/s12882-024-03505-1. https://pubmed.ncbi.nlm.nih.gov/38413872/
5. Lee, Young Ho, Bae, Sang-Cheol. 2014. Association between the functional ITGAM rs1143679 G/A polymorphism and systemic lupus erythematosus/lupus nephritis or rheumatoid arthritis: an update meta-analysis. In Rheumatology international, 35, 815-23. doi:10.1007/s00296-014-3156-2. https://pubmed.ncbi.nlm.nih.gov/25315704/
6. Fagerholm, S C, MacPherson, M, James, M J, Sevier-Guy, C, Lau, C S. . The CD11b-integrin (ITGAM) and systemic lupus erythematosus. In Lupus, 22, 657-63. doi:10.1177/0961203313491851. https://pubmed.ncbi.nlm.nih.gov/23753600/
7. Ramírez-Bello, Julian, Sun, Celi, Valencia-Pacheco, Guillermo, López-Villanueva, Ricardo F, Nath, Swapan K. 2019. ITGAM is a risk factor to systemic lupus erythematosus and possibly a protection factor to rheumatoid arthritis in patients from Mexico. In PloS one, 14, e0224543. doi:10.1371/journal.pone.0224543. https://pubmed.ncbi.nlm.nih.gov/31774828/
8. Chen, Li, Shangguan, Zhiyang, Dong, Zeya, Ding, Yunyun, Yang, Shulong. 2023. NFIL3 aggravates human coronary artery endothelial cell injury by promoting ITGAM transcription in Kawasaki disease. In Hematology (Amsterdam, Netherlands), 28, 2277502. doi:10.1080/16078454.2023.2277502. https://pubmed.ncbi.nlm.nih.gov/37933872/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen