C57BL/6JCya-Itgb2em1/Cya
Common Name:
Itgb2-KO
Product ID:
S-KO-18416
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Itgb2-KO
Strain ID
KOCMP-16414-Itgb2-B6J-VB
Gene Name
Product ID
S-KO-18416
Gene Alias
2E6; Cd18; LAD; LCAMB; Lfa1; MF17
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgb2em1/Cya mice (Catalog S-KO-18416) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000000299
NCBI RefSeq
NM_008404
Target Region
Exon 4~5
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Itgb2, also known as integrin beta 2, is a gene that plays a crucial role in coordinating extracellular and intracellular signaling. Integrins are known to reshape the tumor microenvironment, and Itgb2 is involved in host defense [1]. It has been associated with pathways such as PI3K/AKT/mTOR, which regulate cellular metabolism and growth [1].
In oral squamous cell carcinoma (OSCC), CAFs with higher Itgb2 expression promoted tumor cell proliferation. Itgb2 regulated the PI3K/AKT/mTOR axis to enhance glycolysis in CAFs, and the lactate produced was absorbed by OSCC cells, which then oxidized NADH in the mitochondrial oxidative phosphorylation system to generate ATP [1]. In non-small cell lung cancer, lncRNA ITGB2-AS1 was found to promote cisplatin resistance by inhibiting ferroptosis via activating the FOSL2/NAMPT axis [2]. In severe acute pancreatitis-related acute lung injury, extracellular vesicles overexpressing ITGAM and ITGB2 could attenuate pulmonary inflammation and endothelial cell barrier disruption [3]. In cerebral ischemia-reperfusion injury, Itgb2+ microglia subclusters were involved in energy metabolism, cell cycle, angiogenesis, neuronal myelin formation, and repair at different time points [4]. In glioblastoma, ITGB2 in microglia promoted the mesenchymal transition of GBM cells through the JAK1/STAT3/IL-6 signaling feedback [5]. In diabetic nephropathy, ITGB2 was identified as a potential diagnostic marker [6]. In inflammatory bowel disease, suppression of ITGB2 expression alleviated inflammation in mice, and it may be a potential diagnostic marker for IBD-associated colorectal cancer [7]. In BAP1-mutated uveal melanoma, the ITGB2-ICAM1 axis promoted liver metastasis by preserving hypoxia-and ECM-related signatures [8]. In gliomas, ITGB2 was elevated in higher-malignancy tumors, predicted poor prognosis, and high ITGB2 expression was associated with a better immunotherapy response [9]. In premature infants with necrotizing enterocolitis, although ITGB2 variants were not associated with NEC in the entire cohort, there was a trend towards enrichment with NEC severity in extremely low birthweight infants [10].
In conclusion, Itgb2 is essential in various biological processes and diseases. Studies using different experimental models, though not always gene-knockout or conditional-knockout mouse models, have revealed its role in tumor progression, drug resistance, inflammation-related injuries, and neurodegenerative and kidney diseases. These findings contribute to understanding the molecular mechanisms underlying these diseases and may provide potential therapeutic targets.
References:
1. Zhang, Xiaoxin, Dong, Yingchun, Zhao, Mengxiang, Hu, Qingang, Ni, Yanhong. 2020. ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system. In Theranostics, 10, 12044-12059. doi:10.7150/thno.47901. https://pubmed.ncbi.nlm.nih.gov/33204328/
2. Chen, Huiyong, Wang, Linhui, Liu, Jingting, Liao, Hongliang, Wan, Renping. . LncRNA ITGB2-AS1 promotes cisplatin resistance of non-small cell lung cancer by inhibiting ferroptosis via activating the FOSL2/NAMPT axis. In Cancer biology & therapy, 24, 2223377. doi:10.1080/15384047.2023.2223377. https://pubmed.ncbi.nlm.nih.gov/37370246/
3. Hu, Qian, Zhang, Shu, Yang, Yue, Lyon, Christopher J, Wan, Meihua. 2023. Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury. In ACS nano, 17, 7562-7575. doi:10.1021/acsnano.2c12722. https://pubmed.ncbi.nlm.nih.gov/37022097/
4. Zeng, Fanning, Cao, Jun, Hong, Zexuan, Zou, Xin, Tao, Tao. 2023. Single-cell analyses reveal the dynamic functions of Itgb2+ microglia subclusters at different stages of cerebral ischemia-reperfusion injury in transient middle cerebral occlusion mice model. In Frontiers in immunology, 14, 1114663. doi:10.3389/fimmu.2023.1114663. https://pubmed.ncbi.nlm.nih.gov/37063847/
5. Xie, Han, Jiang, Yanyi, Xiang, Yufei, Tian, Dasheng, Bian, Erbao. . Super-enhancer-driven LIF promotes the mesenchymal transition in glioblastoma by activating ITGB2 signaling feedback in microglia. In Neuro-oncology, 26, 1438-1452. doi:10.1093/neuonc/noae065. https://pubmed.ncbi.nlm.nih.gov/38554116/
6. Xu, Mingming, Zhou, Hang, Hu, Ping, Liu, Li, Liu, Xiaoqiang. 2023. Identification and validation of immune and oxidative stress-related diagnostic markers for diabetic nephropathy by WGCNA and machine learning. In Frontiers in immunology, 14, 1084531. doi:10.3389/fimmu.2023.1084531. https://pubmed.ncbi.nlm.nih.gov/36911691/
7. Xu, Rong, Du, Wei, Yang, Qinglong, Du, Ashuai. . ITGB2 related to immune cell infiltration as a potential therapeutic target of inflammatory bowel disease using bioinformatics and functional research. In Journal of cellular and molecular medicine, 28, e18501. doi:10.1111/jcmm.18501. https://pubmed.ncbi.nlm.nih.gov/39088353/
8. Li, Jiaoduan, Cao, Dongyan, Jiang, Lixin, Zhuang, Ai, Xiang, Dongxi. 2023. ITGB2-ICAM1 axis promotes liver metastasis in BAP1-mutated uveal melanoma with retained hypoxia and ECM signatures. In Cellular oncology (Dordrecht, Netherlands), 47, 951-965. doi:10.1007/s13402-023-00908-4. https://pubmed.ncbi.nlm.nih.gov/38150154/
9. Xu, Houshi, Zhang, Anke, Han, Xiaying, Wang, Wei, Lou, Meiqing. 2021. ITGB2 as a prognostic indicator and a predictive marker for immunotherapy in gliomas. In Cancer immunology, immunotherapy : CII, 71, 645-660. doi:10.1007/s00262-021-03022-2. https://pubmed.ncbi.nlm.nih.gov/34313821/
10. George, Lovya, Menden, Heather, Xia, Sheng, Farrow, Emily, Sampath, Venkatesh. . ITGB2 (Integrin β2) Immunomodulatory Gene Variants in Premature Infants With Necrotizing Enterocolitis. In Journal of pediatric gastroenterology and nutrition, 72, e37-e41. doi:10.1097/MPG.0000000000002941. https://pubmed.ncbi.nlm.nih.gov/32925548/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen