C57BL/6JCya-Faap24em1/Cya
Common Name:
Faap24-KO
Product ID:
S-KO-18454
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Faap24-KO
Strain ID
KOCMP-101831-Faap24-B6J-VA
Gene Name
Product ID
S-KO-18454
Gene Alias
C230052I12Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Faap24em1/Cya mice (Catalog S-KO-18454) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032704
NCBI RefSeq
NM_178643
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
FAAP24, a Fanconi anemia core complex protein, is crucial for DNA damage repair [3,6]. It forms a stable complex with FANCM, anchoring the FA core complex to chromatin in the repair of DNA interstrand crosslinks, and is involved in the Fanconi anemia pathway [3,5]. This interaction with FANCM also aids in remodeling stalled replication forks to facilitate ATR signaling activation in response to replication stress [2].
Isogenic models for FAAP24 have shown that it promotes ATR-mediated checkpoint activation, especially in response to DNA cross-linking agents [4]. In AML, high FAAP24 expression is associated with poor prognosis and the shaping of an immunosuppressive tumor microenvironment, suggesting it could serve as a prognostic biomarker and play an immunomodulatory role [1].
In conclusion, FAAP24 is essential for DNA damage repair and ATR-mediated checkpoint activation. Its role in AML prognosis and immunomodulation, as revealed through functional studies including gene-related models, provides valuable insights into disease mechanisms, potentially guiding future research and treatment strategies for AML and other related diseases.
References：
1. Bao, Xiebing, Chi, Jingyun, Zhu, Yiwei, Xu, Ting, Liu, Song-Bai. 2023. High FAAP24 expression reveals poor prognosis and an immunosuppressive microenvironment shaping in AML. In Cancer cell international, 23, 117. doi:10.1186/s12935-023-02937-3. https://pubmed.ncbi.nlm.nih.gov/37328842/
2. Horejŝí, Zuzana, Collis, Spencer J, Boulton, Simon J. 2009. FANCM-FAAP24 and HCLK2: roles in ATR signalling and the Fanconi anemia pathway. In Cell cycle (Georgetown, Tex.), 8, 1133-7. doi:. https://pubmed.ncbi.nlm.nih.gov/19282663/
3. Ali, Abdullah Mahmood, Singh, Thiyam Ramsing, Meetei, Amom Ruhikanta. 2009. FANCM-FAAP24 and FANCJ: FA proteins that metabolize DNA. In Mutation research, 668, 20-6. doi:10.1016/j.mrfmmm.2009.04.002. https://pubmed.ncbi.nlm.nih.gov/19379763/
4. Wang, Yucai, Leung, Justin W, Jiang, Yingjun, Wang, Weidong, Li, Lei. 2013. FANCM and FAAP24 maintain genome stability via cooperative as well as unique functions. In Molecular cell, 49, 997-1009. doi:10.1016/j.molcel.2012.12.010. https://pubmed.ncbi.nlm.nih.gov/23333308/
5. Yang, Hui, Zhang, Tianlong, Tao, Ye, Tong, Liang, Ding, Jianping. 2013. Structural insights into the functions of the FANCM-FAAP24 complex in DNA repair. In Nucleic acids research, 41, 10573-83. doi:10.1093/nar/gkt788. https://pubmed.ncbi.nlm.nih.gov/24003026/
6. Ciccia, Alberto, Ling, Chen, Coulthard, Rachel, Wang, Weidong, West, Stephen C. . Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM. In Molecular cell, 25, 331-43. doi:. https://pubmed.ncbi.nlm.nih.gov/17289582/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen