C57BL/6JCya-Mybl2em1/Cya
Common Name:
Mybl2-KO
Product ID:
S-KO-18503
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mybl2-KO
Strain ID
KOCMP-17865-Mybl2-B6J-VA
Gene Name
Product ID
S-KO-18503
Gene Alias
B-Myb; Bmyb
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mybl2em1/Cya mice (Catalog S-KO-18503) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018005
NCBI RefSeq
NM_008652
Target Region
Exon 5
Size of Effective Region
~0.2 kb
Detailed Document
Overview of Gene Research
MYBL2, also known as B-MYB, is a transcription factor of the MYB transcription factor family. It is essential in regulating vital cellular processes such as cell proliferation, differentiation, DNA repair, and cell cycle progression. It also plays a role in cell survival [2]. Dysregulation of these processes is associated with cancer development, making MYBL2 a gene of significant interest in cancer research. Genetically engineered mouse models can be valuable tools for studying MYBL2's functions.
In various cancers, MYBL2 shows oncogenic properties. In bladder cancer, MYBL2 deficiency suppressed cell proliferation and migration in vitro and in vivo, while overexpression had the opposite effect. MYBL2 could bind to and transactivate the promoter of CDCA3, promoting the malignant phenotype of bladder cancer cells [1]. In prostate cancer, genetic inhibition of Mybl2 in murine prostate cancer cell lines representing phenotypic plasticity decreased in vivo growth, cell fitness, and repressed gene expression signatures related to pluripotency and stemness [3]. In ovarian cancer, tumor-derived MYBL2 indicated poor prognosis, and its inhibition could reprogram the tumor microenvironment and reduce anti-PD-1 resistance [4].
In conclusion, MYBL2 is a central regulator of cell-related processes. Through model-based research, especially gene knockout (KO) or conditional knockout (CKO) mouse models, its role in promoting cancer progression in multiple cancer types has been revealed. These findings contribute to understanding the mechanisms of cancer development and offer potential therapeutic targets for treating related diseases.
References:
1. Liu, Wei, Shen, Dexin, Ju, Lingao, Xiao, Yu, Wang, Xinghuan. 2022. MYBL2 promotes proliferation and metastasis of bladder cancer through transactivation of CDCA3. In Oncogene, 41, 4606-4617. doi:10.1038/s41388-022-02456-x. https://pubmed.ncbi.nlm.nih.gov/36071275/
2. Musa, Julian, Aynaud, Marie-Ming, Mirabeau, Olivier, Delattre, Olivier, Grünewald, Thomas Gp. 2017. MYBL2 (B-Myb): a central regulator of cell proliferation, cell survival and differentiation involved in tumorigenesis. In Cell death & disease, 8, e2895. doi:10.1038/cddis.2017.244. https://pubmed.ncbi.nlm.nih.gov/28640249/
3. German, Beatriz, Alaiwi, Sarah A, Ho, Kun-Lin, Lawrenson, Kate, Ellis, Leigh. . MYBL2 Drives Prostate Cancer Plasticity: Inhibiting Its Transcriptional Target CDK2 for RB1-Deficient Neuroendocrine Prostate Cancer. In Cancer research communications, 4, 2295-2307. doi:10.1158/2767-9764.CRC-24-0069. https://pubmed.ncbi.nlm.nih.gov/39113611/
4. Pan, Baoyue, Wan, Ting, Zhou, Yun, Li, Jundong, Zheng, Min. 2023. The MYBL2-CCL2 axis promotes tumor progression and resistance to anti-PD-1 therapy in ovarian cancer by inducing immunosuppressive macrophages. In Cancer cell international, 23, 248. doi:10.1186/s12935-023-03079-2. https://pubmed.ncbi.nlm.nih.gov/37865750/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen