C57BL/6JCya-Pxnem1/Cya
Common Name:
Pxn-KO
Product ID:
S-KO-18532
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pxn-KO
Strain ID
KOCMP-19303-Pxn-B6J-VA
Gene Name
Product ID
S-KO-18532
Gene Alias
Pax
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pxnem1/Cya mice (Catalog S-KO-18532) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000212819
NCBI RefSeq
NM_001403066
Target Region
Exon 6
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Pxn, also known as paxillin, is a key component of the mechanotransducing machinery. It is involved in focal adhesion assembly, integrin signaling, and cell-matrix connection. Pxn is related to various cellular functions such as cell contraction, migration, and is also associated with autophagy-related pathways [2,3,4].
In a DDX17-induced DDX17HKO mouse model, knockout of DDX17, which is related to PXN-AS1 alternative splicing, led to a significant reduction in lung metastasis. This indicates that the DDX17-PXN-AS1 axis may play a role in HCC metastasis [1]. In ovarian cancer, high expression of PXN promoted cell proliferation, migration, and invasion, potentially by suppressing the p110β/Vps34/Beclin1 pathway [4]. In breast cancer cells, ULK1/2-mediated phosphorylation of PXN at S32 and S119 weakened its homotypic interactions and liquid-liquid phase separation, impairing focal adhesion assembly and cell migration [2].
In conclusion, Pxn plays essential roles in cell-matrix interactions, mechanotransduction, and autophagy-related pathways. Mouse models, such as the DDX17HKO mouse model, have helped to reveal its significance in cancer metastasis. In ovarian and breast cancers, Pxn is involved in promoting or suppressing cell migration and proliferation, highlighting its importance in understanding cancer progression mechanisms.
References：
1. Zhou, Hong-Zhong, Li, Fan, Cheng, Sheng-Tao, Huang, Ai-Long, Chen, Juan. 2021. DDX17-regulated alternative splicing that produced an oncogenic isoform of PXN-AS1 to promote HCC metastasis. In Hepatology (Baltimore, Md.), 75, 847-865. doi:10.1002/hep.32195. https://pubmed.ncbi.nlm.nih.gov/34626132/
2. Liang, Peigang, Zhang, Jiaqi, Wu, Yuchen, Kundu, Mondira, Wang, Bo. 2023. An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration. In EMBO reports, 24, e56850. doi:10.15252/embr.202356850. https://pubmed.ncbi.nlm.nih.gov/37846507/
3. Liang, Peigang, Wu, Yuchen, Zheng, Shanyuan, Xing, Qiong, Wang, Bo. 2024. Paxillin phase separation promotes focal adhesion assembly and integrin signaling. In The Journal of cell biology, 223, . doi:10.1083/jcb.202209027. https://pubmed.ncbi.nlm.nih.gov/38466167/
4. Wu, Qiaoling, Cui, Zhaolei, Jiang, Shan, You, Yiqing, Sun, Yang. 2023. Autophagy-related gene PXN as a prognostic marker: Promotion of ovarian cancer progression by suppressing the p110β/Vps34/Beclin1 pathway. In Cell biochemistry and function, 41, 599-608. doi:10.1002/cbf.3815. https://pubmed.ncbi.nlm.nih.gov/37232085/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen