C57BL/6JCya-Ppp1cbem1/Cya
Common Name:
Ppp1cb-KO
Product ID:
S-KO-18672
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ppp1cb-KO
Strain ID
KOCMP-19046-Ppp1cb-B6J-VA
Gene Name
Product ID
S-KO-18672
Gene Alias
1200010B19
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1cbem1/Cya mice (Catalog S-KO-18672) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015100
NCBI RefSeq
NM_172707
Target Region
Exon 4~6
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Ppp1cb, also known as the beta-type catalytic subunit of serine/threonine-protein phosphatase 1, is involved in multiple biological processes. It participates in the RAS/MAPK pathway, and is associated with the development of certain developmental disorders. Additionally, it plays a role in adipogenesis, hepatic de novo lipogenesis, and may be related to the regulation of glycolysis in lung adenocarcinoma [1,2,4,5,6].
Mutations in Ppp1cb have been identified in patients with Noonan syndrome-like disorders. For instance, a patient with Noonan syndrome had a known de novo PPP1CB variant, and an individual with a p.Pro49Arg mutation in PPP1CB presented with features similar to Noonan syndrome-like disorder with loose anagen hair and craniosynostosis [1,3]. In 3T3-L1 preadipocytes, depletion of PPP1CB suppressed differentiation into mature adipocytes, suggesting its role as an adipogenic activator [5,6]. In the context of insulin resistance in mice, WDR6 interacts with PPP1CB to enhance fatty acid synthases transcription, promoting hepatic de novo lipogenesis [2].
In conclusion, Ppp1cb is essential in various biological processes such as development, adipogenesis, and lipid metabolism. Its involvement in diseases like Noonan syndrome-related disorders and potential implications in metabolic and cancer-related conditions, as revealed through patient-based and cell-based studies, highlight its significance. Understanding Ppp1cb may offer insights into the mechanisms of these diseases and potential therapeutic strategies.
References:
1. Umeki, Ikumi, Niihori, Tetsuya, Abe, Taiki, Kure, Shigeo, Aoki, Yoko. 2018. Delineation of LZTR1 mutation-positive patients with Noonan syndrome and identification of LZTR1 binding to RAF1-PPP1CB complexes. In Human genetics, 138, 21-35. doi:10.1007/s00439-018-1951-7. https://pubmed.ncbi.nlm.nih.gov/30368668/
2. Yao, Zhenyu, Gong, Ying, Chen, Wenbin, Bo, Tao, Zhao, Jiajun. 2023. Upregulation of WDR6 drives hepatic de novo lipogenesis in insulin resistance in mice. In Nature metabolism, 5, 1706-1725. doi:10.1038/s42255-023-00896-7. https://pubmed.ncbi.nlm.nih.gov/37735236/
3. Bertola, Débora, Yamamoto, Guilherme, Buscarilli, Michelle, Passos-Bueno, Maria Rita, Kim, Chong. . The recurrent PPP1CB mutation p.Pro49Arg in an additional Noonan-like syndrome individual: Broadening the clinical phenotype. In American journal of medical genetics. Part A, 173, 824-828. doi:10.1002/ajmg.a.38070. https://pubmed.ncbi.nlm.nih.gov/28211982/
4. Zhou, Yanling, Peng, Xintong, Fang, Cheng, Shi, Ying, Liu, Shuang. 2024. Histones Methyltransferase NSD3 Inhibits Lung Adenocarcinoma Glycolysis Through Interacting with PPP1CB to Decrease STAT3 Signaling Pathway. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2400381. doi:10.1002/advs.202400381. https://pubmed.ncbi.nlm.nih.gov/39119928/
5. Kim, Jinsoo, Ahn, Dohee, Chung, Sang J. 2022. Chebulinic Acid Suppresses Adipogenesis in 3T3-L1 Preadipocytes by Inhibiting PPP1CB Activity. In International journal of molecular sciences, 23, . doi:10.3390/ijms23020865. https://pubmed.ncbi.nlm.nih.gov/35055051/
6. Cho, Young-Lai, Min, Jeong-Ki, Roh, Kyung Min, Chung, Sang J, Kang, Hyo Jin. 2015. Phosphoprotein phosphatase 1CB (PPP1CB), a novel adipogenic activator, promotes 3T3-L1 adipogenesis. In Biochemical and biophysical research communications, 467, 211-7. doi:10.1016/j.bbrc.2015.10.004. https://pubmed.ncbi.nlm.nih.gov/26449462/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen