C57BL/6JCya-Sp110em1/Cya
Common Name:
Sp110-KO
Product ID:
S-KO-18675
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Sp110-KO
Strain ID
KOCMP-109032-Sp110-B6J-VA
Gene Name
Product ID
S-KO-18675
Gene Alias
5031415C07Rik; 52kDa; 5830484A20Rik; Ifi75; Ipr1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sp110em1/Cya mice (Catalog S-KO-18675) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093508
NCBI RefSeq
NM_030194
Target Region
Exon 4~11
Size of Effective Region
~14.4 kb
Detailed Document
Overview of Gene Research
SP110, also known as Speckled 110, is an interferon-responsive nuclear body protein. It has critical roles in cell cycling, apoptosis, and immunity to infection. It may function as a nuclear hormone receptor transcriptional coactivator and is expressed in human peripheral blood leukocytes and spleen [8].
Regarding tuberculosis (TB), studies on SP110 gene polymorphisms have shown inconsistent results. Some meta-analyses found no significant association between certain polymorphisms (rs1135791, rs9061, rs11556887, rs3948464, rs1346311) and TB susceptibility, while others indicated that SNP rs9061 might be a risk factor for TB. In the Vietnamese population, certain SP110 variants were associated with both pulmonary and extra-pulmonary TB. In a Mongolian population, the rs722555 SNP in SP110 was associated with an increased risk of TB. In Taiwanese populations, several SNPs in SP110 were associated with susceptibility to latent and active TB infection [1,4,3,5,7]. In macrophages, depletion of SP110 and/or SP140 impaired the induction of gene expression associated with inflammatory responses, including IFN response genes during Mycobacterium tuberculosis infection, suggesting that SP110 acts as a positive regulator for these genes [2]. In mouse macrophages, Sp110 interacts with heat shock protein 5 (Hspa5) to activate endoplasmic reticulum (ER) stress-induced apoptosis, enhancing macrophage resistance to Mycobacterium tuberculosis [6].
In conclusion, SP110 is crucial in immunity-related processes, especially in the context of Mycobacterium tuberculosis infection. Studies on SP110 gene polymorphisms and functional studies in macrophages and mouse models have provided insights into its role in TB susceptibility and the associated immune responses. These findings contribute to understanding the host-pathogen interactions and may potentially guide the development of strategies related to TB prevention and treatment.
References:
1. Lei, Xun, Zhu, Hang, Zha, Lang, Wang, Yang. 2012. SP110 gene polymorphisms and tuberculosis susceptibility: a systematic review and meta-analysis based on 10 624 subjects. In Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 12, 1473-80. doi:10.1016/j.meegid.2012.05.011. https://pubmed.ncbi.nlm.nih.gov/22691368/
2. Nakamura, Hajime, Hikichi, Haruka, Seto, Shintaro, Hijikata, Minako, Keicho, Naoto. 2024. Transcriptional regulators SP110 and SP140 modulate inflammatory response genes in Mycobacterium tuberculosis-infected human macrophages. In Microbiology spectrum, 12, e0010124. doi:10.1128/spectrum.00101-24. https://pubmed.ncbi.nlm.nih.gov/39162523/
3. Cui, Xiaogang, Yuan, Tianqi, Ning, Pengyuan, Xing, Li, Wu, Changxin. 2022. Polymorphisms in the ASAP1 and SP110 Genes and Its Association with the Susceptibility to Pulmonary Tuberculosis in a Mongolian Population. In Journal of immunology research, 2022, 2713869. doi:10.1155/2022/2713869. https://pubmed.ncbi.nlm.nih.gov/36249417/
4. Zhang, Shuai, Wang, Xue Bin, Han, Ya Di, Zhou, Ye, Zheng, Fang. . Certain Polymorphisms in SP110 Gene Confer Susceptibility to Tuberculosis: A Comprehensive Review and Updated Meta-Analysis. In Yonsei medical journal, 58, 165-173. doi:10.3349/ymj.2017.58.1.165. https://pubmed.ncbi.nlm.nih.gov/27873510/
5. Fox, Gregory J, Sy, Dinh Ngoc, Nhung, Nguyen Viet, Saunders, Bernadette M, Britton, Warwick J. 2014. Polymorphisms of SP110 are associated with both pulmonary and extra-pulmonary tuberculosis among the Vietnamese. In PloS one, 9, e99496. doi:10.1371/journal.pone.0099496. https://pubmed.ncbi.nlm.nih.gov/25006821/
6. Wu, Yongyan, Guo, Zekun, Liu, Fayang, Luo, Yan, Zhang, Yong. 2017. Sp110 enhances macrophage resistance to Mycobacterium tuberculosis via inducing endoplasmic reticulum stress and inhibiting anti-apoptotic factors. In Oncotarget, 8, 64050-64065. doi:10.18632/oncotarget.19300. https://pubmed.ncbi.nlm.nih.gov/28969051/
7. Chang, So-Yi, Chen, Mei-Ling, Lee, Meng-Rui, Wang, Jann-Yuan, Yan, Bo-Shiun. 2018. SP110 Polymorphisms Are Genetic Markers for Vulnerability to Latent and Active Tuberculosis Infection in Taiwan. In Disease markers, 2018, 4687380. doi:10.1155/2018/4687380. https://pubmed.ncbi.nlm.nih.gov/30627224/
8. Bloch, D B, Nakajima, A, Gulick, T, de La Monte, S M, Bloch, K D. . Sp110 localizes to the PML-Sp100 nuclear body and may function as a nuclear hormone receptor transcriptional coactivator. In Molecular and cellular biology, 20, 6138-46. doi:. https://pubmed.ncbi.nlm.nih.gov/10913195/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen