C57BL/6JCya-Gabarapem1/Cya
Common Name
Gabarap-KO
Product ID
S-KO-18713
Backgroud
C57BL/6JCya
Strain ID
KOCMP-56486-Gabarap-B6J-VB
Status
When using this mouse strain in a publication, please cite “Gabarap-KO Mouse (Catalog S-KO-18713) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gabarap-KO
Strain ID
KOCMP-56486-Gabarap-B6J-VB
Gene Name
Product ID
S-KO-18713
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000018711
NCBI RefSeq
NM_019749
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Overview of Gene Research
GABARAP, short for GABA type A receptor-associated protein, belongs to the LC3/GABARAP protein family evolved from yeast Atg8. It plays crucial roles in autophagy, a process maintaining cellular homeostasis by degrading and recycling aged and cytotoxic components. In autophagy, GABARAP is involved in steps like cargo recognition, vesicle closure, and trafficking. It also has functions in non-autophagy-related mechanisms [1].
Loss of GABARAP in multiple myeloma cells mediates resistance to immunogenic chemotherapy. Specifically, GABARAP deletion impairs the exposure of calreticulin (CRT) on dying cells, reducing phagocytosis by dendritic cells and the subsequent antitumor T-cell response. Low GABARAP levels are associated with shorter survival in patients and reduced tumor immune infiltration [2].
In conclusion, GABARAP is essential for autophagy-related and non-autophagy-related functions. In multiple myeloma, its loss-of-function significantly impacts the efficacy of immunogenic chemotherapy, highlighting its importance in understanding the disease mechanism and potentially developing new treatment strategies.
References：
1. Schaaf, Marco B E, Keulers, Tom G, Vooijs, Marc A, Rouschop, Kasper M A. 2016. LC3/GABARAP family proteins: autophagy-(un)related functions. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30, 3961-3978. doi:. https://pubmed.ncbi.nlm.nih.gov/27601442/
2. Gulla, Annamaria, Morelli, Eugenio, Johnstone, Megan, Munshi, Nikhil C, Anderson, Kenneth C. . Loss of GABARAP mediates resistance to immunogenic chemotherapy in multiple myeloma. In Blood, 143, 2612-2626. doi:10.1182/blood.2023022777. https://pubmed.ncbi.nlm.nih.gov/38551812/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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