C57BL/6NCya-Btnl2em1/Cya
Common Name:
Btnl2-KO
Product ID:
S-KO-18889
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Btnl2-KO
Strain ID
KOCMP-547431-Btnl2-B6N-VB
Gene Name
Product ID
S-KO-18889
Gene Alias
BTL-II; BTLN2; Btl2; Gm315; NG9
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Btnl2em1/Cya mice (Catalog S-KO-18889) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025198
NCBI RefSeq
NM_079835
Target Region
Exon 1~3
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Btnl2, or butyrophilin-like protein 2, is a molecule belonging to the B7 family. It is known to regulate T cell activation and has been associated with multiple biological processes and diseases. It is involved in pathways related to immune responses, with its mutation linked to inflammatory autoimmune diseases such as sarcoidosis and myositis [4].
In mice, Btnl2 knockout (KO) studies have shown its significance. Btnl2-KO mice display decreased colonic tumorigenesis and more severe colitis phenotypes due to defective production of IL-22, as Btnl2 acts on group 3 innate lymphoid cells (ILC3s), CD4+ T cells, and γδ T cells to promote IL-22 production. Additionally, antibody-mediated blockade of Btnl2 attenuates tumour progression in murine tumour models, reducing the number of tumour-infiltrating IL-17A-producing γδ T cells and myeloid-derived suppressor cells while facilitating cytotoxic CD8+ T cell accumulation [1,2]. Also, in non-obese diabetic (NOD) mice, in vivo administration of rBTNL2-Ig (a recombinant form) ameliorates type 1 diabetes (T1D), associated with inhibiting autoreactive T cell functions and increasing regulatory T cells generation [3].
In conclusion, Btnl2 plays a crucial role in regulating immune-related biological processes. The gene knockout mouse models have revealed its importance in diseases like colitis-associated tumorigenesis, tumour immune escape, and T1D, highlighting its potential as a therapeutic target for these and other related diseases.
References:
1. Peng, Qianwen, Pan, Ting, He, Ruirui, Du, Yanyun, Wang, Chenhui. 2023. BTNL2 promotes colitis-associated tumorigenesis in mice by regulating IL-22 production. In EMBO reports, 24, e56034. doi:10.15252/embr.202256034. https://pubmed.ncbi.nlm.nih.gov/36629012/
2. Du, Yanyun, Peng, Qianwen, Cheng, Du, Zhang, Zhanguo, Wang, Chenhui. 2022. Cancer cell-expressed BTNL2 facilitates tumour immune escape via engagement with IL-17A-producing γδ T cells. In Nature communications, 13, 231. doi:10.1038/s41467-021-27936-8. https://pubmed.ncbi.nlm.nih.gov/35017553/
3. Tian, Xiaohong, Lin, Yujun, Cui, Cheng, Su, Min, Lai, Laijun. 2019. BTNL2-Ig Protein Attenuates Type 1 Diabetes in Non-Obese Diabetic (NOD) Mice. In Advanced healthcare materials, 8, e1800987. doi:10.1002/adhm.201800987. https://pubmed.ncbi.nlm.nih.gov/30884196/
4. Nguyen, Thang, Liu, Xikui K, Zhang, Yongliang, Dong, Chen. . BTNL2, a butyrophilin-like molecule that functions to inhibit T cell activation. In Journal of immunology (Baltimore, Md. : 1950), 176, 7354-60. doi:. https://pubmed.ncbi.nlm.nih.gov/16751379/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen