C57BL/6JCya-Bap1em1/Cya
Common Name:
Bap1-KO
Product ID:
S-KO-19272
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Bap1-KO
Strain ID
KOCMP-104416-Bap1-B6J-VA
Gene Name
Product ID
S-KO-19272
Gene Alias
2300006C11Rik; mKIAA0272; uch-x4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bap1em1/Cya mice (Catalog S-KO-19272) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022458
NCBI RefSeq
NM_027088
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
BAP1, short for BRCA1-Associated Protein 1, is a ubiquitin carboxy-terminal hydrolase. As a tumor suppressor, it uses its deubiquitinating activity to regulate multiple processes like DNA damage repair, cell cycle control, chromatin modification, programmed cell death, and the immune response [1,3,5]. It is also involved in metabolic regulation of ferroptosis [2].
In CRISPR-engineered human cholangiocyte organoids, BAP1 loss-of-function led to loss of epithelial characteristics and increased motility, and restoring its catalytic activity in the nucleus rescued these changes. In human liver organoids with combined common cholangiocarcinoma mutations, BAP1 loss resulted in acquisition of malignant features upon xenotransplantation, suggesting that BAP1 controls epithelial identity through chromatin accessibility regulation [4].
In conclusion, BAP1 is crucial in maintaining normal cellular functions, especially in processes related to tumor suppression. Its role in regulating epithelial identity and involvement in various cancer-related processes, such as in cholangiocarcinoma, are well-demonstrated through organoid-based functional studies. These findings enhance our understanding of BAP1's function and its implications in cancer development and treatment.
References:
1. Louie, Bryan H, Kurzrock, Razelle. 2020. BAP1: Not just a BRCA1-associated protein. In Cancer treatment reviews, 90, 102091. doi:10.1016/j.ctrv.2020.102091. https://pubmed.ncbi.nlm.nih.gov/32877777/
2. Zhang, Yilei, Shi, Jiejun, Liu, Xiaoguang, Li, Wei, Gan, Boyi. 2018. BAP1 links metabolic regulation of ferroptosis to tumour suppression. In Nature cell biology, 20, 1181-1192. doi:10.1038/s41556-018-0178-0. https://pubmed.ncbi.nlm.nih.gov/30202049/
3. Masclef, Louis, Ahmed, Oumaima, Estavoyer, Benjamin, Nijnik, Anastasia, Affar, El Bachir. 2021. Roles and mechanisms of BAP1 deubiquitinase in tumor suppression. In Cell death and differentiation, 28, 606-625. doi:10.1038/s41418-020-00709-4. https://pubmed.ncbi.nlm.nih.gov/33462414/
4. Artegiani, Benedetta, van Voorthuijsen, Lisa, Lindeboom, Rik G H, Vermeulen, Michiel, Clevers, Hans. 2019. Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. In Cell stem cell, 24, 927-943.e6. doi:10.1016/j.stem.2019.04.017. https://pubmed.ncbi.nlm.nih.gov/31130514/
5. Kwon, Jongbum, Lee, Daye, Lee, Shin-Ai. 2023. BAP1 as a guardian of genome stability: implications in human cancer. In Experimental & molecular medicine, 55, 745-754. doi:10.1038/s12276-023-00979-1. https://pubmed.ncbi.nlm.nih.gov/37009801/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen